Publications by authors named "Chethan K Krishna"
Front Cell Dev Biol
August 2023
Article Synopsis
- PEX19 binding sites are crucial for the proper targeting and localization of PEX11, a key peroxisomal and glycosomal membrane protein.
- There are two main PEX19 binding sites in PEX11, with the N-terminal site being highly conserved and necessary for its effective targeting, while the second site promotes glycosomal localization.
- Mutations or deletions in these binding sites can cause mislocalization of PEX11 to mitochondria, and the N-terminal region of PEX11 harbors a signal that redirects it to mitochondria when glycosomal transport is impaired.
Methods Mol Biol
March 2023
Article Synopsis
- * Since glycosomes are unique to these parasites, they are of significant interest for research in parasite biology and potential treatments.
- * The text outlines a method for isolating highly purified glycosomes and enriching their membrane proteins from the Trypanosoma brucei, involving techniques like cell rupture, differential centrifugation, and various buffer extractions.
