Replacement of fishmeal with a microbial single-cell protein induced enteropathy and poor growth outcomes in barramundi (Lates calcarifer) fry.

Fish meal (FM) replacement is essential for the sustainable expansion of aquaculture. This study focussed on the feasibility of replacing FM with a single-cell protein (SCP) derived from methanotrophic bacteria (Methylococcus capsulatus, Bath) in barramundi fry (Lates calcarifer). Three isonitrogenous and isoenergetic diets were formulated with 0%, 6.

A retrospective study of pathologic findings in cephalopods (extant subclasses: Coleoidea and Nautiloidea) under laboratory and aquarium management.

This retrospective study examines pathologic findings in 593 captive cephalopods (340 octopuses, 130 cuttlefish, 33 squid, and 90 nautiluses; 22 species in total) submitted to International Zoo Veterinary Group Pathology between May 2003 and August 2022. Common octopus, European common cuttlefish, hummingbird bobtail squid, and chambered nautilus were the most numerous species from the included orders of Octopoda, Sepiida, Sepiolida, and Nautilida, respectively. Commonly identified conditions included coccidiosis and renal dicyemid mesozoan infection in octopuses, amebiasis in squid, bacterial infections in cuttlefish, and idiopathic multisystemic inflammatory disease in nautiluses.

Evolutionarily Young African Rhinoceros Gammaretroviruses.

High-throughput sequences were generated from DNA and cDNA from four Southern white rhinoceros () located in the Taronga Western Plain Zoo in Australia. Virome analysis identified reads that were similar to endogenous gammaretrovirus (McERV). Previous analysis of perissodactyl genomes did not recover gammaretroviruses.

A RETROSPECTIVE REVIEW OF POST-METAMORPHIC MOUNTAIN CHICKEN FROG () NECROPSY FINDINGS FROM EUROPEAN ZOOLOGICAL COLLECTIONS, 1998 TO 2018.

The mountain chicken frog () is the largest endemic amphibian species in the Western Hemisphere. Since 1998, this critically endangered species has been maintained as a European Endangered Species Programme, but low breeding success and a high mortality rate threaten the sustainability of the captive frog population. In the current study, we analyzed gross and histopathologic postmortem information from 212 mountain chicken frogs that died in European zoological collections from 1998 to 2018.

MAST CELL TUMORS IN CHEETAH (): A CASE SERIES.

Mast cell tumors in nondomestic felids are rarely reported and their biological characteristics are not well described. A retrospective review of the pathology records of 52 zoo-housed cheetahs () identified five cases of mast cell tumor, involving four closely related individuals. The age at initial presentation varied from 14 mo to 6 yr.

Colonic malignant peripheral nerve sheath tumour in a cat.

Case Summary: A 14-year-old male neutered domestic mediumhair cat presented with a 4 month history of inappetence and weight loss. Pertinent abnormalities on haematology and biochemistry included a mild microcytic regenerative anaemia (packed cell volume [PCV] 24% [reference interval (RI) 30-45%], mean cell volume 30.8 fl [RI 40-45 fl], absolute reticulocyte count 326.

Molecular Diagnosis of Felis catus Gammaherpesvirus 1 (FcaGHV1) Infection in Cats of Known Retrovirus Status with and without Lymphoma.

The pathogenicity of gammaherpesvirus 1 (FcaGHV1), a common infection of domestic cats, is unknown. To explore an association between FcaGHV1 detection and feline lymphoma, a retrospective, cross-sectional, disease-association study was conducted. The infection status of all cats for feline immunodeficiency virus and feline leukaemia virus was determined.

Deep-sequencing to resolve complex diversity of apicomplexan parasites in platypuses and echidnas: Proof of principle for wildlife disease investigation.

The short-beaked echidna (Tachyglossus aculeatus) and the platypus (Ornithorhynchus anatinus) are iconic egg-laying monotremes (Mammalia: Monotremata) from Australasia. The aim of this study was to demonstrate the utility of diversity profiles in disease investigations of monotremes. Using small subunit (18S) rDNA amplicon deep-sequencing we demonstrated the presence of apicomplexan parasites and confirmed by direct and cloned amplicon gene sequencing Theileria ornithorhynchi, Theileria tachyglossi, Eimeria echidnae and Cryptosporidium fayeri.

CALVARIAL OSTEOSARCOMA WITH CEREBRAL COMPRESSION IN A FREE-RANGING POWERFUL OWL (NINOX STRENUA).

A free-ranging powerful owl (Ninox strenua) presented in a dull state with extensive bruising of the skin overlying the skull, due to suspected trauma. Supportive care was provided, which resulted in the return to a normal state, although intermittent subtle neurologic abnormalities remained. One month from original presentation, intermittent episodes of head turning and a possible seizure were noted, but behavior and appetite were otherwise normal.

Hematologic and Biochemical Reference Values of the Australian Bush Rat (Rattus fuscipes).

We provide hematologic (n = 34) and biochemical (n = 30) blood values for wild-caught Australian bush rats (Rattus fuscipes). Hematology values have similar range limits compared with other rat species. Biochemistry values for glucose, alanine transaminase, aspartate aminotransferase, and creatine kinase have higher maximum ranges compared with other rats.

Acute lymphoblastic leukemia in a pygmy hippopotamus (Hexaprotodon liberiensis).

A captive, 31-yr-old, intact male pygmy hippopotamus presented with nonspecific signs of weight loss, inappetence, diarrhea, and lethargy. After 5 wk of diagnostic investigation and symptomatic treatment, an acute leukemic process with concurrent polycystic kidney disease was suspected. The animal's condition continued to deteriorate prompting euthanasia.

Dilated cardiomyopathy in a De Brazza's monkey (Cercopithecus neglectus).

Background: Cardiomyopathies have been reported in many primates. They may result from an inflammatory response to an infectious agent, nutritional deficiency, familial-genetic inheritance or toxic agents, but in many cases they are idiopathic.

Methods: A De Brazza's monkey (Cercopithecus neglectus) presented with weight loss and inappetence.

Neosporosis in an aborted southern white rhinoceros (Ceratotherium simum simum) fetus.

In December 2008, a southern white rhinoceros (ãsimum simum) aborted a 7-mo gestation male fetus. On hematoxylin and eosin-stained sections of fetal tissues, foci of necrosis were noted in the hepatic parenchyma and were associated with low numbers of lymphocytes, plasma cells, and neutrophils. Protozoal zoites were identified within the hepatic lesions and within the cerebellum.

Feasibility of using coyotes (Canis latrans) as sentinels for bovine mycobacteriosis (Mycobacterium bovis) infection in wild cervids in and around Riding Mountain National Park, Manitoba, Canada.

Elk (Cervus elaphus manitobensis) and white-tailed deer (Odocoileus virginianus) in the Riding Mountain National Park (RMNP) region of southwestern Manitoba have been identified as a likely wildlife reservoir of Mycobacterium bovis, the causative agent of bovine mycobacteriosis in livestock. The feasibility of using coyotes (Canis latrans) collected from trappers as a sentinel species was investigated. Retropharyngeal, mesenteric, and colonic lymph nodes and tonsils collected at necropsy from 82 coyotes were examined by bacterial culture, polymerase chain reaction (PCR), and acid-fast histopathology.

Signaling to the enemy? Body pattern expression and its response to external cues during hunting in the cuttlefish Sepia officinalis (Cephalopoda).

Cuttlefish can rapidly alter their appearance by using neurally controlled chromatophore organs. This ability may provide a window into their cognitive capacity. We test whether the changes in body pattern that occur during hunting depend on context.

Ovarian remnant syndrome in a 5-year-old bitch.

A 5-year-old German shepherd bitch was presented with a 3-week history of vaginal bleeding. Ovarian remnant syndrome was diagnosed on vaginoscopy, vaginal cytology, serum progesterone levels, ultrasonography, and exploratory laparotomy. The condition resolved following surgical excision of the remaining ovarian tissue.