[Peripheral pool of CD8+ T-cells contains lymphocytes with antigen-specific receptors that recognize syngeneic MHC class II molecules].

The capacity of T-lymphocytes to recognize "nonself" and tolerating "self" is formed as a result of positive and negative selection in the thymus. While obtaining and testing specificity of T-hybridomas, we demonstrated that the major part of peripheral pool of CD8+ T-lymphocytes carried receptors specific to "self" MHC class II molecules. Such an unexpected specificity of receptors has been found in some T-cell hybridomas produced by fusion of activated peripheral CD8+ T-lymphocytes with a tumor partner transfected by the coreceptor CD4 gene.

[Monoclonal antibodies to the influenza A virus matrix protein that react with viral strains of different subtypes].

To obtain hybridomas secreting monoclonal antibodies (MCA) to matrix (M) protein of influenza virus, mice were immunized with a modified antigen which consisted of subvirus units after electrophoretic removal of surface glycoproteins from virions using Desintegnon-O detergent. Six stable hybrid cultures producing MCA to M-protein were derived. The properties of MCA to the antigen determinant common for a group of influenza A virus strains of different subtypes allow them to be used in the development of a test-system for diagnosis of influenza A type.

[Molecular and genetic approaches to the study of the regulation of the function of T-suppressor cells specific for histocompatibility molecule epitopes].

Using the model of T-suppression (Ts) induction by i/v injection of irradiated allogeneic splenocytes we were able to show that such specific Ts (1) have their own precursors and express unique membrane markers; that (2) the genetic restriction of Ts/responder interaction (interactional restriction) is based on the direct contact of an Ia molecule on Ts membrane and a putative syngeneic anti-Ia receptor, which appears on the membrane of responder lymphocyte after its activation by an allo-antigen; and that (3) the Ts receptors unlike that of other T-subsets recognize simple serologically defined determinants in the context of an MHC molecule. The practical application of the information of the Ts properties is discussed.

[Isolation of monoclonal antibodies to horseradish peroxidase and their use in immunohistochemistry and immunoblotting].

Monoclonal anti-peroxidase antibodies (McAb) were generated by means of hybridization of BALB/c immune splenocytes with X-63.653 cells. Peroxidase-antiperoxidase (PAP) staining of fibroblast cultures with murine McAb against fibronectin was used for the detection of positive cultures.

[Spectrum of reactivity of monoclonal antibodies against specific T-suppressors].

Monoclonal antibodies (MoAb) against specific T-suppressors CI and C4 are characterized by their reactivity with normal lymphoid cells and some tumour cell lines cultivated in vitro. MoAb CI and C4 react with T and B cells from spleen and lymph nodes. The amount of CI and C4 T and B subsets are equal in the spleen (25-29%), while lymph node T-lymphocytes contain twice as much CI and C4 cells than B-lymphocytes (40 and 20%, respectively).

[In vivo induction of cytotoxic T lymphocytes in H-2Kbm mutant mice and cross-reactivity of narrowly specific killer clones].

Anti-wild-type (B6) H-2Kbm mutant (bm) CTL were induced in the regional lymph nodes by 2 injections (with 2 week interval) of bm mice into foot-pads with B6 irradiated splenocytes. CTL were tested 7 days after the boost, including 3 days precultivation in monoculture (required for high CTL activity in bm). Active bm4 CTL inducible in vivo but not in the mixed lymphocyte culture (MLC), while bm1, bm3 and their F1 hybrids with BALB/c were equally active in both models.

[Induction in vivo of specific T suppressors in mice of mutant lines H-2KbT].

The differences in the generation of specific suppressor T cells (SSTC) against H-2Kb wild type were investigated in H-2Kbm1, H-2Kbm3 and H-2Kbm4 mutants. Anti-Kb SSTC were produced only by bm3 mutant and F1(BALB/c X bm3) hybrid. T-cell nature of SSTC of bm3 mutant was confirmed by anti-Thy 1.

[Production of monoclonal antibodies to antigens of specific mouse T-suppressors].

F1(MSU X WAG) rats were immunized with anti B6 BALB/c specific suppressor T cells (SSTC), purified by absorption/elution technique, with the following fusion of splenocytes to NS-I myeloma cell line. Hybrids were screened for their ability to affect SSTC, cytotoxic T lymphocytes (CTL) and producers of macrophage migration inhibition factor (MIF-producers) all triggered by in vivo priming with allogeneic cells. Two hybridoma cell lines--C1 and C4 inactivated SSTC by approximately 50%, leaving CTL and MIF-producers intact.

[Characteristics of 2 types of antigen-binding T suppressors formed during an immune response using T-T hybridomas and their extracts].

Antigen-binding T cells of mice immunized with low doses of syngenic spleen cells modified by 2,4,6-trinitrophenyl sulphonic acid, were fused with BW 5147.3.13 thymoma subclone.

[Detection and isolation of antibody-forming clones by means of local cytolysis in gel].

A method is suggested combining the screening and cloning of hybridomas producing cytotoxic antibodies. The method is based on the Erne local hemolysis principles. The cells from the preformed hybridoma line NATF 9.

[Use of monoclonal antibodies against the light-chain immunoglobulins of the rat in the radioimmune analysis of differentiated lymphocyte antigens of the mouse].

Spleen cells from SJL mice immunized with rat immunoglobulin light chains were fused with X-63 myeloma. Seven hybridoma lines were obtained. Monoclonal antibodies (McAB) secreted by lines L1G9, L2B2 and L3E8 were purified and labeled by 125I.

[Isolation of monoclonal antibodies to antigen Lyt-3.2].

A hybridoma producing monoclonal antibodies (McAb) NATF9.9 (F9) was obtained from fusion of murine myeloma X63 and splenocytes of AKR mice immunized with a single intravenous injection of 5 X 10(7) thymocytes of CBA mice. F9 McAb were cytotoxic for 80% thymocytes, 10% splenocytes, 20% lymph node cells, 85% cortical and 32% medullary thymocytes of CBA, C57BL/6, BALB/c, DBA/2 and SJL but not for the cells of C58 and AKR mice.

[Kinetic differences in induction of the killers and producers of the macrophage migration inhibitory factor (MIF) in primary and secondary mixed murine leukocyte culture test].

Conditions are selected for the killer induction during primary and secondary responses in the one-way MLC, stimulated by x-irradiated or killed allogenic lymphocytes. MIF is found in the MLC culture medium as a sharp peak on the 2nd or the 3rd day of the primary reaction, or as a plato from the 1st day of the secondary reaction. In contrast, the killers are shown to be induced much later reaching their maximum on the 5th and the 4th days, respectively.