The microbiome of oral cavity patients with periodontitis, adhesive and biofilm forming properties.

The work characterizes the intestinal microbiota of patients with ischemic stroke, including the spectrum, frequency and number of microorganisms, as well as the spectrum and amount of gas signaling molecules secreted by lactobacilli. It was found that in patients with ischemic stroke, the frequency of the main representatives of normal microflora, Bifidobacterium spp., Lactobacillus spp.

The microbiome of oral cavity patients with periodontitis, adhesive and biofilm forming properties.

The microbiome of oral cavity in healthy people and patients with periodontitis was analyzed to determine their adhesive properties and the ability to form biofilms. The study involved 2 groups: healthy, 18 people, and an experimental group, 20 patients with chronic generalized periodontitis moderate severity of the disease. The average age of the studied people was 35-45 years.

Peculiarities of the mouth and colum in the youth of annous age with arterial hypertension and metabolic disorders.

The purpose of the work is to determine the species, quantitative composition and frequency of occurrence of microorganisms in the oral fluid and large intestine in healthy young men and having arterial hypertension, and metabolic disorders. A survey was conducted of 51 young students studying at Tver State Medical University. It was found that more than 70% of students with AH (arterial hypertension) and MBN (metabolic disorders) showed pronounced dysbiotic disorders of the intestinal microbiota of II and III degree with a decrease in the number of Lactobacillus spp.

[The state and role of esophagus, stomach, intestinal microbiota in patients with ulcer disease, chronic gastritis, esophagitis.].

The microflora of 64 biopsies taken during fibrogastroduodenoscopy of the mucous membrane of the esophagus, stomach and duodenum in healthy volunteers and 1120 samples obtained from the same parts of the digestive tract in patients with esophagitis, chronic gastritis and peptic ulcer disease were studied. The patients ranged in age from 18 to 62 years. Traditional bacteriological method was used to isolate and identify microorganisms.

[Dynamics of variability of microbiotes of the cavity of the mouth and the great intestinent of young men when changing the conditions of life.].

The purpose of the work is to determine the spectrum, quantity and frequency of occurrence of microorganisms in the oral fluid and large intestine in healthy young men under changes of living conditions. A survey was conducted of 14 nonresident young men, students of the 1st and 2nd courses, studying at Tver State Medical University, which included questions about the place of birth, living conditions, the presence of chronic diseases and the frequency of their exacerbations over the past year, water and food regimes, physical exertion, the presence of bad habits and emotional stress, etc. The oral fluid and feces were examined to determine the spectrum and amount of microbiota of the upper and lower parts of the gastrointestinal tract.

[Gastrointestinal microbiota of the newborns of the first month of life of the Tver region.].

The purpose of our work was to identify the features of the newborn´s microbiota formation during first month of their life on the model of the Tver region. The samples of oral fluid, meconium and feces of the first month newborns were examined in 38 full-term and 21 premature children of both sexes to determine the spectrum and quantity of oral fluid and intestine microorganisms. As the result, it was found that in full-term and vaginally delivered infants the microbiota of the oral cavity and intestine was characterized by greater diversity than in children delivered by C-section, in which Lactobacilli, Bifidobacteria, E.

[Optimization of wound healing by correction of the oral microbiota at the stage of surgical treatment of tongue cancer].

Post-resection wound healing, oral microbiota composition and saliva lysozyme activity were assessed in 36 patients with tongue carcinoma before and after surgery with the local application of 2% solution of acid-diluted chitosan in the postoperative period. The microbiocenosis of the oral cavity before treatment in cancer patients was represented by conditionally pathogenic and pathogenic bacteria, whose number is more than 4 lg CFU/cm, indicating dysbiosis; the lysozyme level was reduced, accounting for 25.4±8.

Adaptation and Probiotic Potential of Lactobacilli, Isolated from the Oral Cavity and Intestines of Healthy People.

The present study shows that, from 300 Lactobacillus strains isolated from the oral cavity and large intestine of 600 healthy people, only 9 had high antagonistic activity against pathogens and opportunistic pathogens. All antagonistic strains of lactobacilli have been identified by 16S rRNA sequencing and assigned to four species: Lactobacillus fermentum, Lactobacillus rhamnosus, Lactobacillus plantarum, and Lactobacillus casei. In addition, these lactobacilli appeared to be nonpathogenic and had some probiotic potential: the strains produced lactic acid and bacteriocins, showed high sensitivity to broad-spectrum antibiotics, and were capable of forming biofilms in vitro.

[THE ROLE OF HELICOBACTER PYLORI IN NORMOMICROBIOCENOSIS AND DYSBACTERIOSIS OF MUCOSAL MICROFLORA OF OESOPHAGOGASTRODUODENAL ZONE IN THE CASES OF PEPTIC ULCER, CHRONIC GASTRITIS AND OESOPHAGITIS].

Aim: Determine the qualitative and quantitative composition of the mucosal microflora of oesophagogastroduodenal zone to determine the location of Helicobocter pylori and its place in normomicrobiocenosis and dysbacteriosis in cases of peptic ulcer, chronic gastritis and oesophagitis.

Materials And Methods: Clinical and microbiological studies were conducted in 30 healthy individuals-volunteers, 130 patients with peptic ulcer, 36--chronic gastritis and 24--chronic esophagitis.

Results Of The Study: Helicobacter pylori in 33% of cases included in normomicrobiocenosis of mucosal microflora oesophagogastroduodenal zone, which consists of 12 genera of microorganisms and carries out all protection functions.

[The effect of glucose level in blood on microbiocenosis of intestine and quality of life of people with predisposition to diabetes mellitus type II.].

The article presents analysis of results of examination of spectrum, rate of occurrence and number of microorganisms of large intestine in individuals with normal level of glucose in blood and with predisposition to diabetes mellitus type II. The psychodiagnostic study was carried out including estimate of particular psycho-physiologic and social psychological characteristics of personality reflecting characteristics of psychic and social development. It is established that in individuals with predisposition to diabetes mellitus type II microbiota of intestine altered sideway to dysbiosis degree I-II at the expense of increasing of number of opportunistic microflora up to several degrees.

[THE MICRO-ECOLOGY OF DIGESTIVE TRACT AS AN INDICATOR OF HUMAN HEALTH CONDITIONS].

The study was carried out to analyze qualitative and quantitative parameters of oral fluid and feces in 74 healthy individuals of different age groups. In most of the cases, alterations of micro-ecology are established characterizing by decreasing of amount of indigenous micro-flora and increasing of number of opportunistic pathogenic microorganisms of genera of Staphylococcus, Bacillus, Candida. The degree of evidence of these alterations reliably increases with age.

[Microbiological aspects of zirconia orthopedic structures].

The article gives a detailed analysis of oral cavity microbiological aspects of 23 patients before and after prosthetic treatment with zirconia structures. Microbiocenoses of oral cavity in patients with defects of the dentition can be described as disbiotic. It was found that the incidence of lactobacilli and bifidobacteria was increased and frequency of occurrence of pathogens, including Staphylococcus aureus, was decreased under the influence of temporary plastic prosthesis as an intermediate step prosthetics.

[Microbiocenosis of major biotopes of the oral cavity in patients with tongue carcinoma during complex treatment].

The composition of the microflora in three biotopes of the oral cavity was examined in 36 patients with tongue carcinoma before chemotherapy, one - two weeks after two courses of chemotherapy and before gemiglosseklomiya. The oral dysbiosis was revealed in patients before antitumor therapy. The opportunistic and pathogenic bacteria in concentration more than 4 lg CFU/cm² were dominant in microbiocenosis of oral cavity.

[Role of viruses with oncogenic potential and their associations in oncogenesis in maxillofacial area].

The potential role of viruses with oncogenic potential such as human papilloma virus, cytomegalovirus, Epstein-Barr virus, herpes simplex virus type 1 and 2, herpes virus type 6, in the development of benign and pre-cancerous tumors of maxillofacial region was assessed in the study. We examined 26 patients with tumors in maxillofacial region (skin and mucosa) using molecular-genetic and histological studies of surgically removed neoplasms removed. In 53.

[Microbiological monitoring of oral fluid of clinically healthy children].

The article presents qualitative and quantitative parameters of microbiocenosis of oral fluid and feces of clinically healthy children in different age groups. In most of the cases compensated disbiotic changes were found, which were characterized by reduction in the number of indigenous microflora (lactobacilli and bifidobacteria) and increase of representatives of opportunistic pathogens (Staphylococcus, Bacillus and Candida). Microecological changes in different gastrointestinal biotopes are closely interrelated.

[Gastroesophageal reflux disease associated with duodenogastroesophageal reflux in patients with biliary pathology: the specific features of the course and esophagogastroduodenal microbial biocenosis].

Aim: To study the specific features of the clinical course of gastroesophageal reflux disease (GERD) associated with duodenogastroesophageal reflux (DGER) in patients with chronic acalculous cholecystitis (CAC) and cholelithiasis (CL), as well as qualitative and quantitative characteristics.

Subjects And Methods: The clinical, morphological, motor tonic characteristics of the esophagogastroduodenal area, mucosal microbial biocenosis in the esophagus, stomach, and duodenum were studied in detail in 83 patients with GERD that was associated with DGER and ran concurrently with CAC or CL.

Results: Impaired duodenal propulsive activity as a concomitance of the signs of gastrostasis and duodenal dyskinesia with dyscoordination of both anthroduodenal and duodenojejunal propulsion and with the development of duodenogastric reflux and DGER, which in turn determine esophageal and gastric pH values is shown to be of importance in CAC and CL, which match GERD.

[The microbiocenosis of upper parts of digestive tract of children with gastroesophageal reflux disease and chronic gastroduodenitis].

The article considers the microbe specter of mucous coat of esophagus and stomach in children with gastro esophageal reflux disease and chronic gastroduodenitis. In patients with diseases of upper parts of digestive tract, the opportunistic pathogenic bacteria are isolated more often and their strains have aggression factors and cytotoxicity. In children with reflux disease the microbe landscape of esophagus is larger both in numerical and specific respect as compared to healthy adolescents and children with isolated gastroduodenitis.

[The disbiotic changes of intestines microflora in healthy people].

The article presents the results of study of species and quantitative composition of intestinal flora in volunteer students aged 18-21 years. The disbiotic abnormality of different degrees was diagnosed in 75% of students. The associations of microorganisms, in case of normobiosis and disbacteriosis degree I included predominantly representatives of normoflora, in case of disbiotic abnormality II and III degrees included both representatives of normoflora and opportunistic pathogenic microorganisms.

[The microbiocenosis of intestine and immune status of children of primary school age].

The article deals with the study of species and quantitative structure of microbiocenosis of intestine and characteristics of immune status in children aged 8-10 years. In children with chronic tonsillitis pharyngitis and bronchitis and with diseases of gastro-intestinal tract (biliary dysfunction, chronic gastroduodenitis and gastritis) the microbe imbalance of various degree of manifestation was established which was prevailing in cases of children with pathology of gastrointestinal tract. The increase of quantity of opportunistic microflora induces the production of both immunoglobulins and cytotoxic lymphocytes and cells-natural killers.

[Biofilm formation by antagonistic strains of oral Lactobacillus].

Highantagonistic strains of lactobacillus isolated from the oral cavity of healthy people, genetically identified as L. fermentum 39, L. rhamnosus 50, L.

[Clinical effect of chitosan in bacterial vaginosis therapy].

Aim: Determination of antibacterial activity of acid-soluble chitosan in bacterial vaginosis therapy in women of reproductive age and comparison of therapy results of traditional scheme and local administration of acid-soluble chitosan.

Materials And Methods: Vaginal microflora of 76 gynecologic and obstetrical female patients (18-43 years of age) with bacterial vaginosis diagnosis was examined by using bacteriological method. Control group consisted of 30 practically healthy women.

[Antibacterial activity of nanostructured silver gel].

Aim: Evaluation of antimicrobial activity of L-cysteine silver gel against various species of pathogenic and opportunistic microorganisms.

Materials And Methods: Antibacterial activity of L-cysteine silvergel with silver concentration from 1.28 x 10(-3) to 3.

[Genetic identification and study of the ability of lactobacilli isolated from the oral cavity of healthy individuals to form biofilms].

The highly antagonistic lactobacillus strains isolated from the oral cavity of human individuals were genetically passported as L. fermentum 39, L. rhamnosus 50, and L.

[Ability of human oral cavity indigenous lactobacilli to form biofilms].

Aim: To determine ability of lactobacilli strains indigenous for oral cavity of healthy persons to adhesion, autoaggregation, surface hydrophobicity, coaggregation and formation of biofilms.

Materials And Methods: Twenty-three anatagonistically active isolates of lactobacilli, including 5 strains of Lactobacillus plantarum, 6--L. rhamnosus, 4--L.

[Genetic identification of antagonistically active lactobacillus strains isolated from the oral cavity of healthy individuals].

Examination of dental deposits from 45 healthy individuals detected 3 lactobacillus strains showing a high antagonism toward test cultures. The api 50 CH "bio Merieux" test systems were employed to identify strains as Lactobacillus fermentum 39, Lactobacillus rhamnosus 24 and Lactobacillus paracasei 50. The results of analyzing the sequences of the 16S rRNA genes of the test strains confirmed this identification, except for the latter strain.