[Increase of probability of cell loss in the endothelium of blood vessel capillaries in progeny of rats after a low-dose irradiation of one of the parents (electron microscopy study)].

The total gamma-irradiation of Wistar rats at a dose of 0.25 Gy as well as at higher doses (0.5, 2 and 4.

[Structural changes in platelets in alloxan diabetes as parameters of their activation in the vascular bed and morphologic features].

The present study shows that a stable level of blood platelet number persists in diabetic rats (following 15 and 60 days after experimental alloxan injection) Unusual platelet megaforms are seen accumulating (up to 36%), having no size analogs in control animals. A quantitative electron-microscopic analysis demonstrated that the enlargements of diabetic platelets was accompanied by their rounding in shape and by a more frequent (by 2.0-2.

[A functional-morphological study of the mucociliary system at stages in the disease in patients with chronic bronchitis].

Combined morpho-functional study of the mucociliary system in patients with chronic bronchitis at different stages of its development was made. Regional mucociliary clearance was evaluated by dynamic aerosol scintigraphy and electron microscopy. Radiography of bronchial biopsies was performed, rheological properties of bronchial content were also determined.

[Pseudomutagenesis. The persistent increase in the level of cellular variability induced by radiation and some other agents].

Electron microscopic investigation of capillary endothelium cells in myocardium of irradiated rats revealed an unusual effect of persistent increase of probability of cell damage, similar in many relations to that described earlier for various unicellular species. New effect, unlike those traditionally studied, is characterised by non-stochastic nature, large number of the involved cells, reveals itself even after faint influences, and can be induced as well by agents other than radiation. The question is put on the probable commonness between these changes and the pseudomutagenesis which has the similar phenomenology.

[The action of dipyridamole on megakaryocytopoiesis in regenerating and stationary bone marrow cell populations].

The effect of dipyridamole on megakaryocytopoiesis in regenerating and stationary populations of mouse bone marrow cells has been studied by heterotopic transplantation of the bone marrow using histological, electron microscopic and biochemical techniques. It is shown that drug administration induced destruction of megakaryocytes. In megakaryocytic cytoplasm giant lipid granules were found whose growth and number increase resulted in megakaryocytes kill.

[Content of trace elements and steroid hormones in blood and tissues of internal organs of rats during an inflammatory process in the bronchi].

Prolonged interrupted inhalation of nitrogen dioxide in a dose of 36.0 +/- 2.2 mg/m3 was attended by definite morphological signs of inflammation in the lungs of rats (hyperplasia of goblet cells, thickening of the basilar membrane, differentiation of ciliated epithelium, etc.

[Problems of lung transplantation. The state of the mucous membrane of the bronchial tree in acute ischemia of the lung].

The results of the work showed the importance of developing methods for the prevention of ischemic damage to the bronchi and optimal methods for reinforcement of the bronchial suture in lung transplantation. Ischemia may be an important pathogenetic factor of incompetence of the bronchial suture in lung transplantation and may also facilitate disturbance of the transplant's mucociliary clearance with the development of postoperative atelectases, dystelectases, and bronchopneumonia. These data are based on the results of experiments obtained in mongrel dogs by electron microscopy.

[Pulmonary histiocytosis X].

Morphologic studies of the lungs of 61 patients aged 15-62 years with pulmonary histiocytosis X revealed a different morphologic picture in relation to the duration of the disease. The most prevalent were primary and chronic forms characterized by the presence of histiocytic granulomas of varying size with blue-green inclusions, eosinophilia and corpuscles X in the macrophage cytoplasm. As the process progressed, fibrobullous changes occurred in the lungs.

[Routes of protein transport into the lungs from the bronchial vascular system].

In order to study the role of the bronchial vascular system in pulmonary protein transport, experiments have been performed in 6 anesthesized dogs, 16 isolated canine lungs and 10 white rats. When bilateral ligation of the bronchial arteries is performed in the anesthesized dogs, cessation of the bronchial blood stream results in decreasing protein transport and lymph outflow from the lungs. In the isolated canine lungs perfused through the pulmonary and bronchial vessels, lymph formation is determined by presence of the bronchial perfusion.

[Hamman-Rich syndrome].

An analysis of 29 observations of the Hamman-Rich syndrome was carried out. Histological, histochemical, and electron microscopic studies showed the earliest changes to consist in edema of the interstitial connective tissue of the lungs followed by the development of crude lesions of alveolar elasticity and increase in the number of argirophilic and collagen fibers leading to disorders in the aerohematic barrier. The chronic course of the disease leads to pulmonary microcystosis.

[Submicroscopic and immunochemical study of horseradish peroxidase passage through the epithelium of the bronchial mucosa].

2% solution of horseradish peroxidase (HRP) was placed on the bronchial mucosa of anaesthetized dogs through a bronchoscope. Arterium femoralis and thracal lymphatic duct were canulated to HRP application. The radiate immunoprecipitation method was utilized to assess HRP activity in blood and lymph samples.

[Epithelial permeability study of the bronchi in chronic bronchitis using an electron-dense tag].

An electron dense tracer, horseradish peroxidase (HRP), treated with DAB-H2O2 for electron histochemical demonstration of peroxidase was used in an effort to find out whether or not the human bronchial epithelium was permeable to some macromolecules in chronic bronchitis. The biopsy specimens obtained from 5 patients at bronchoscopy and cultured in a medium with 2% HRP for 1-6 hours showed the reaction product to be distributed within intracellular spaces of the epithelium as well as in some vesicles and vacuoles of the apical portion of the ciliated cells. When 2% HRP solution was topically applied on the bronchial epithelium surface of a narcotized patient for 10 min followed by biopsy of this part of the epithelium fixation and treatment for HRP demonstration, electron dense precipitates were identified only within some intercellular spaces.

[Submicroscopical study of the granule-containing cells of bronchial carcinoids].

The submicroscopical structure of three bronchial carcinoids was studied. In all the cases, in the cytoplasm of tumor cells secretory granules formed from cisterns of the Golgi apparatus were found. Two types of granules were observed.