Ocular abscess: An extremely rare complication of transurethral resection of the prostate.

Ocular abscess following transurethral resection of the prostate is an rare complication. We report the case of a male who developed an ocular abscess with purulent melting of the eye after undergoing TURP for benign prostatic hyperplasia. The patient successfully underwent the TURP procedure.

Penile gangrene due to improper application of a condom catheter: a case report.

Background: Fournier's gangrene is a rare, fulminant, and usually localized necrotizing soft tissue polymicrobial infection of the perineum, with occasional extension up to the abdominal wall.

Case Presentation: We describe an unusual case of penile gangrene in a 64-year-old Tunisian man suffering from urinary incontinence secondary to cerebrovascular accident. Gangrene developed due to continuous tourniquet effect on the penis caused by a condom catheter.

Optimising the dose of oral midazolam sedation for dental procedures in children: a prospective, randomised, and controlled study.

Background: Midazolam sedation poses a significant dilemma in paediatric dentistry, which is to find out the optimal dosing with minimal undesirable adverse events. In this study, we aimed to compare the effect of three doses of oral midazolam (0.5, 0.

Trends of incident dialysis patients in Tunisia between 1992 and 2001.

Background: Reliable information about the burden of end-stage renal disease (ESRD) is still scarce in the developing world.

Study Design: Observational study of a national dialysis registry.

Setting & Participants: Patients incident to dialysis therapy captured in the Tunisian registry of renal replacement therapy (RRT).

[Study of contents of nitrates, fluorides, aluminium in dialysis water in Tunisia].

Nitrates, fluorides and aluminum have often been incriminated in cases of poisoning sometimes life threatening such as methaemoglobinaemia, dental and skeletal fluorosis, and myoclonal encephalopathy in patients undergoing haemodialysis. The three elements mentioned have something in common: their water origin (drinking water and dialysis water). To study the situation in Tunisia in this respect we propose to determinate the contents of nitrates, fluorides, and aluminum in drinking water and dialysis water supplied by the water board (SONEDE).

[The new information technologies in medicine: what about the patient?].

The automation of the medical records involves many ethical and complex questions totally out of suspicion two decades ago. Effectively, the patient being in the core of the medical information system belonging to any medical structure, it should be evident that this system generates much more information than any classical medical record. This new approach in dealing with medical information has certainly multiple advantages because it helps, according to the case, gather all the information about the patient.

[Primary lymphoma of bone: imaging features].

Primary lymphoma of bone (PLB) has benefited from recent advances in medical imaging and immunohistochemical study that are of prognostic and therapeutic value. From 3 case reports of PLB involving the iliac bone and the skull base, we review the definition of PLB and their main features. The importance of complete initial workup for staging in order to exclude secondary bone lymphomas, more frequent and of worse prognosis, is emphasized.

[Health care supply regulation].

In the current context of health systems' reforms, the regulation would have to occupy a major place in the new role of the State concerning health in order that the system could reach the essential objective to control the quantity and the distribution of health services and to insure an optimal, efficient and equitable coverage of the population's needs. Authors pass in review, in a first part, current regulation means of health care supply, public and private, put in place in Tunisia and suggest their development in areas not covered yet; notably these in relation with the quality and the cost-containment of health care. The current regulation is interested especially in areas of the infrastructure, equipment, medicines and pharmaceutical products and financial and human resources of the sector.

[Comparative study of localization of tryptophanyl-tRNA-synthetase and components of high molecular weight aminoacyl-tRNA-synthetase complex in animal cells].

A comparative study on the localization of cytosolic Trp-tRNA synthetase (TrpRS), aminoacyl-tRNA synthetases associated in a multienzyme complex (Glu-tRNA synthetase (GluRS), and Arg-tRNA synthetase (ArgRS)) and polypeptides p37 and p43 from the multienzyme complex was carried out on ultrathin sections of cultured rabbit cells RK-1 by means of immunogold technique. It is shown that GluRS, ArgRS, and polypeptide p43 have approximately the same distribution in the cell as TrpRS. The data obtained evidences in favour of a multienzyme structure of most (or, may be all) aminoacyl-tRNA synthetases in intact cells.

[The use of RNAse A-colloidal gold complexes for research on RNA-containing cell structures: the effect of different factors on the specificity of the labelling].

Effects of various factors on the specificity and intensity of labelling of RNA-containing structures on the ultrathin sections by RNase A--colloidal gold complexes were studied. The data obtained show that at the optimal choice and standard conditions of labelling the reproducibility of the results is achieved up to 10-20%. It makes it possible to use RNase A-gold method for the quantitative analysis of RNA distribution in the cells at various stages of cell cycle.

[Immune electron microscope determination of the localization of tryptophanyl-tRNA-synthetase in bacteria and higher eukaryotes].

Localization of tryptophanyl-tRNA synthetase (TRS) was studied on ultrathin (UT) sections of Escherichia coli cells and of rat fibroblasts fixed with glutaraldehyde and embedded in "Lowicryl K4M" resin at -35 degrees C. The UT sections were treated with the complexes of monoclonal and/or polyclonal antibodies against TRS with colloidal gold 15 and 8 nm in size. In both types of the cells cytoplasm was the most intensely labelled.

[Isolation and fractionation of the nuclear matrix from cultured cells].

A new method for the isolation of tissue culture cell nuclei is presented which involves incubation of the nuclei in the presence of Cu2+- or Zn2+-ions. This method eliminates the danger of nuclear aggregation and permits nuclear matrix isolation and subsequent fractionation. Stabilization of the inner matrix by Cu2-ions permits analysis of the role of nucleic acids in the maintenance of the matrix structure.

[Electron microscope study of changes in the chromatin structure in different functional states of nuclei of a ciliate Bursaria truncatella and a myxomycete Physarum polycephalum].

Chromatin structural organization was studied by means of electron microscopy in the macronuclei of ciliate Bursaria truncatella at various stages of the life cycle (at different time intervals after cell division, in resting cysts and at excysting) and in the nuclei of myxomycete Physarum polycephalum during the mitotic cycle. Inactive chromatin was shown to be organized in compact clumps 100-300 nm in diameter linked with each other, their loop organization being convincingly demonstrated. Upon activation chromatin decompacts and is represented by nucleosomal fibres with a lot of replicationally and transcriptionally active regions.

[Residual chromatin of mouse spleen nuclei after intensive nuclease treatment. Isolation and properties].

Two-step treatment of mouse spleen nuclei with staphylococcal nuclease was used to isolate residual nuclear structures lacking a considerable part of chromation. Partial disruption of the nuclear envelope after the first step of digestion was shown to be essential for obtaining residual nuclear structures. Isolated residual nuclear structures contained condensed chromatin (residual chromatin) which was not solubilized upon additional staphylococcal nuclease treatment and amounted to approximately 20% of total nuclear chromatin.

[Electron microscopic study of the action of submicroscopic levorin structures on Candida guillermondii].

In the course of preparation of aqueous solutions of the polyene antibiotic levorin, the latter is recovered in the solid phase forming granular submicroscopic structures. If the cells of Candida guilliermondii are treated with submicroscopic granular structures (SMGS) of levorin, the structures are adsorbed on the surface of the yeast cell walls. Some visible changes occur in the ultrastructure of the yeast cells incubated with SMGS of levorin for 5 min: the inner layer of the cell wall becomes loose, the periplasmic space appears, the cytoplasmic membrane becomes thicker, the mitochondria swell, and fragmentation of the mitochondrial cristae takes place.

[Fine structure of the outer sheath of the aerial mycelium in Actinomyces levoris].

The outer sheath of the aerial mycelium of Actinomyces levoris 64 bears groove-like and granular submicroscopic structures. They are very susceptible to a short-term treatment of the aerial mycelium with aqueous acetone which causes their disintegration. Under specified conditions, submicroscopic structures, viz.

[Makeup of the tubular structures of a stock culture of Act. roseoflavus var. roseofungini].

The methods of spectral analysis, spectrophotometry, fluorescence and thin-layer chromatography were used for the study of the composition of the tubular structures of the initial culture of Act. roseoflavus var. roseofungini.

[Production of tubular structures from the aerial mycelium of Actinomyces roseoflavus var. roseofungini].

Tubular formations analogous by their structure to those found on the surface of the outer sheath of the hyphae of the aerial mycelium of Actinomyces roseoflavus var. roseofungini were reconstructed in vitro from the mycelium acetone extract on addition of water. A polyenic antibiotic was shown to be present in the composition of the "tubes".

[Conditions for the self-organization of submicroscopic structures from mycelial extracts of the "fructose" variant of Actinomyces roseflavus var. roseofungini].

Self-organization of various submicroscopic formations, such as "tubes", "ribbons", etc. differing in ultrastructure and containing a polyenic antibiotic was observed in acetone extracts of the mycelium of the "fructose" variant of Actinomyces roseoflavus var. roseofungini.

[Self-assembly of extracellular tubular structures of non-protein nature produced by an actinomycete].

When grown on the solid synthetic medium with glucose as the only carbon source the dedifferentiated "fructose" mutant of Actinomyces roseoflavus var. roseofungini accumulated aggregates of tubular-like structures. The individual tubules had the internal diametre of 80 A and external diametre of approximately 200-220 A.