Effect of forskolin on prostaglandin synthesis by mouse resident peritoneal macrophages.

The relationship between cyclic 3',5'-adenosine monophosphate (cAMP) and prostaglandin (PG) synthesis was investigated in the mouse resident peritoneal macrophage. Macrophages were established as monolayer cultures in petri dishes. After addition of various agents, culture media and cells were removed for PG and cAMP analysis by standard radiochromatographic techniques and radioimmunoassay respectively.

Differential effects of putative lipoxygenase inhibitors on arachidonic acid metabolism in cell-free and intact cell preparations.

The effects of nordihydroguairetic acid (NDGA), 3-amino-1-trifluoromethyl-)-phenyl-2-pyrazoline (BW755c), eicostatetraynoic acid (ETYA), phenidone, quercetin, and indomethacin (INDO) on the synthesis of 15-hydroxyeicosatatetraenoic acid (15-HETE) from soybean 15-lipoxygenase, leukotriene B4 (LTB4) from 5-lipoxygenase, and prostaglandin E2 (PGE2) from cyclooxygenase enzymes of rat neutrophils and mouse peritoneal macrophages were investigated. All of the drugs caused a dose-related inhibition of increased oxygen consumption by soybean 15-lipoxygenase in the presence of arachidonic acid and the rank order of potency was phenidone greater than or equal to BW755c greater than ETYA greater than quercetin greater than NDGA greater than indomethacin. The reduction in oxygen consumption correlated with a reduction of 15-HETE formation as identified by high-performance liquid chromatography.