Instant rerouting of photosynthetic electron transport to O reduction after the plasma membrane excitation of Chara in the presence of methyl viologen.

-Action potential (AP) of excitable plant cells is an important signaling event that can differentially alter physicochemical and physiological processes in various parts of the same cell. In giant cells of characean algae, the AP propagation has minor effect on photosynthetic electron transport in areas with high activity of plasmalemmal H-pump but inhibits linear electron flow in regions featuring high passive H/OH conductance of the plasma membrane (PM). Uneven spatial distributions of local periplasmic and cytoplasmic pH facilitate the operation of distinct (CO-dependent and O-mediated) pathways of photoinduced electron flow, which presumably accounts for differential influence of AP on photosynthesis.

Reverse Dynamization Accelerates Regenerate Bone Formation and Remodeling in a Goat Distraction Osteogenesis Model.

This article was updated on December 20, 2023, because of previous errors, which were discovered after the preliminary version of the article was posted online. Figure 4 has been replaced with a figure that presents different p values. Also, on page 1943, the text that had read: "Quantitative microCT confirmed that the total volume of the regenerate in the RD group was much smaller compared with the SF (p = 0.

Contribution of TRPC3-mediated Ca entry to taste transduction.

The current concept of taste transduction implicates the TASR/PLCβ2/IPR3/TRPM5 axis in mediating chemo-electrical coupling in taste cells of the type II. While generation of IP has been verified as an obligatory step, DAG appears to be a byproduct of PIP cleavage by PLCβ2. Here, we provide evidence that DAG-signaling could play a significant and not yet recognized role in taste transduction.

The Relative Age Effect in the Best Track and Field Athletes Aged 10 to 15 Years Old.

(1) The purpose of this study was to examine the prevalence of the relative age effect (RAE) in the best young (10 to 15 years old) track and field athletes. (2) Hypothesis: The prevalence of the RAE in the best young track and field athletes of both genders will be evident in all age groups from 10 to 15 years old, which may be associated with the significant relationship between biological maturity, chronological age, and the development of physical qualities. (3) Materials and methods: In total, 1778 athletes volunteered for this study.

Taste Cells of the Type III Employ CASR to Maintain Steady Serotonin Exocytosis at Variable Ca in the Extracellular Medium.

Type III taste cells are the only taste bud cells which express voltage-gated (VG) Ca channels and employ Ca-dependent exocytosis to release neurotransmitters, particularly serotonin. The taste bud is a tightly packed cell population, wherein extracellular Ca is expected to fluctuate markedly due to the electrical activity of taste cells. It is currently unclear whether the Ca entry-driven synapse in type III cells could be reliable enough at unsteady extracellular Ca.

Comparative Stiffness Characteristics of Ilizarov- and Hexapod-type External Frame Constructs.

Background: The Ilizarov method and fixator are clinically recognised for the treatment of fractures, limb salvage and deformity correction. There have been extensive studies determining the basic mechanism for fracture healing using this technique. It is generally accepted that circular frames optimise the mechanical environment by reducing shear strain across the fracture while maintaining axial micromotion so as to promote fracture healing.

Narrative review of ring fixator management of recurrent club foot deformity.

Despite the widespread use of the Ponseti method for treatment of clubfeet, there continue to be a significant number of patients who present with a severe, stiff clubfoot as a result of extensive intra-articular soft tissue release or lack of access to care. In such patients, circular external fixators can be utilized for deformity correction with distraction across soft tissues, joints, and osteotomies. Ilizarov or hexapod circular fixators may be utilized according to surgeon preference.

Effects of chloroplast-cytoplasm exchange and lateral mass transfer on slow induction of chlorophyll fluorescence in Characeae.

Rapid cytoplasmic streaming in characean algae mediates communications between remote cell regions exposed to uneven irradiance. The metabolites exported from brightly illuminated chloroplasts spread along the internode with the liquid flow and cause transient changes in chlorophyll fluorescence at cell areas that are exposed to dim light or placed shortly in darkness. The largest distance to which the photometabolites can be transported has not yet been determined.

Reverse Dynamization Accelerates Bone-Healing in a Large-Animal Osteotomy Model.

Background: Reverse dynamization is a mechanical manipulation regimen designed to accelerate bone-healing and remodeling. It is based on the hypothesis that a fracture that is initially stabilized less rigidly allows micromotion to encourage initial cartilaginous callus formation. Once substantial callus has formed, the stabilization should then be converted to a rigid configuration to prevent the disruption of neovascularization.

Real-Time Monitoring with a Controlled Advancement Drill May Decrease Plunge Depth.

Background: Although drill use is fundamental to orthopaedic surgery, the risk of plunging past the far cortex and potentially damaging the surrounding soft tissues remains unavoidable with conventional drilling methods. A dual motor drill may decrease that risk by providing controlled drill-bit advancement and real-time monitoring of depth and energy expenditure. We hypothesized that using the dual motor drill would decrease plunge depth regardless of the user's level of experience.

Biomechanical Considerations in Foot and Ankle Circular External Fixation: Maintenance of Wire Tension.

Initial tensioning of the forefoot wires to 130 kg followed by simultaneous tensioning of the calcaneal wires to 90 kg and using the rigid double-row foot plate closed anteriorly via threaded rods produce maximum preservation of the initial wire tension during foot circular external fixation.

Treatment Strategies and Frame Configurations in the Management of Foot and Ankle Deformities.

To provide standardized nomenclature for various hexapod frame configurations for foot and ankle deformity correction, a unique classification of the hexapod external fixators was proposed. This classification is based on number of correction levels, secured anatomic blocks, and direction of the strut attachment. It allows the combination of all different foot and ankle frame assemblies into a few standard hexapod configurations, irrespective of which external fixator is used.

Photoinduction of electron transport on the acceptor side of PSI in Synechocystis PCC 6803 mutant deficient in flavodiiron proteins Flv1 and Flv3.

After transferring the dark-acclimated cyanobacteria to light, flavodiiron proteins Flv1/Flv3 serve as a main electron acceptor for PSI within the first seconds because Calvin cycle enzymes are inactive in the dark. Synechocystis PCC 6803 mutant Δflv1/Δflv3 devoid of Flv1 and Flv3 retained the PSI chlorophyll P700 in the reduced state over 10 s (Helman et al., 2003; Allahverdiyeva et al.

One-year recombinant growth hormone therapy does not improve hemoglobin state and morphology of erythrocytes in growth hormone deficient children.

An increase in growth rates of children suffering from growth hormone deficiency (GHD) subjected to recombinant growth hormone treatment (rGHT) was shown to be accompanied by acceleration of metabolic processes that may stimulate oxygen consumption in various organs and tissues. Therefore, oxygen-transporting properties of RBC should undergo considerable changes during the rGHT. The aim of this study was to examine the effects of rGHT on erythrocyte shape and hemoglobin state in GHD children.

Self-Organization Induced by Self-Assembly in Microheterogeneous Reaction-Diffusion System.

When acrylamide (AA) monomers are added to the Belousov-Zhabotinsky (BZ) reaction incorporated into nanodroplets of water-in-oil aerosol OT (AOT) microemulsion (the BZ-AOT system), free radicals produced in the BZ reaction initiate polymerization of AA monomers and polyacrylamide particles are formed. These particles change the microstructure of the AOT microemulsion thus inducing the transition from Turing patterns to new dissipative patterns which can be either stationary "black" spots or waves.

Deoxygenation Affects Composition of Membrane-Bound Proteins in Human Erythrocytes.

Background/aims: ATP release from erythrocyte plays a key role in hypoxia-induced elevation of blood flow in systematic circulation. We have previously shown that hemolysis contributes to erythrocyte ATP release triggered by several stimuli, including hypoxia, but the molecular mechanisms of hypoxia-increased membrane fragility remain unknown.

Methods: In this study, we compared the action of hypoxia on hemolysis, ATP release and the composition of membrane-bound proteins in human erythrocytes.

Expression of calcium-activated chloride channels Ano1 and Ano2 in mouse taste cells.

Specialized Ca(2+)-dependent ion channels ubiquitously couple intracellular Ca(2+) signals to a change in cell polarization. The existing physiological evidence suggests that Ca(2+)-activated Cl(-) channels (CaCCs) are functional in taste cells. Because Ano1 and Ano2 encode channel proteins that form CaCCs in a variety of cells, we analyzed their expression in mouse taste cells.

The Nature of Foot Ray Deficiency in Congenital Fibular Deficiency.

Background: Absent lateral osseous structures in congenital fibular deficiency, including the distal femur and fibula, have led some authors to refer to the nature of foot ray deficiency as "lateral" as well. Others have suggested that the ray deficiency is in the central portion of the midfoot and forefoot.We sought to determine whether cuboid preservation and/or cuneiform deficiency in the feet of patients with congenital fibular deficiency implied that the ray deficiency is central rather than lateral in patients with congenital fibular deficiency.

Lengthening in Congenital Femoral Deficiency: A Comparison of Circular External Fixation and a Motorized Intramedullary Nail.

Background: Circular external fixation for limb-lengthening is associated with frequent and numerous complications. Intramedullary lengthening devices represent a potential advance in limb-lengthening. The purpose of this study was to compare the outcomes of femoral lengthening in pediatric patients treated by either circular external fixation or a motorized intramedullary nail.

The Management of Soft Tissue and Bone Loss in Type IIIB and IIIC Pediatric Open Tibia Fractures.

Purpose: Type III B and C open tibia fractures in children pose a challenge to the orthopaedic surgeon. Limb salvage is the initial goal for the majority of patients, but managing soft-tissue defects and bone loss can be a challenge. The purpose of this study was to evaluate the use of circular external fixation in the management of these injuries.

Clinical Implications of Psychosocial Factors on Pediatric External Fixation Treatment and Recommendations.

Background: Pediatric limb reconstruction using circular external fixation is a prolonged treatment that interrupts patients' daily function. Patient personality characteristics and expectations may interfere with planned treatment, making complicated medical procedures more challenging. The aims of this study are to identify factors impacting treatment outcome and recommendations for preoperative evaluation and planning.

Evaluation of complications of treatment of severe Blount's disease by circular external fixation using a novel classification scheme.

Corrective osteotomy for recalcitrant varus deformity secondary to adolescent and infantile Blount's disease can be challenging because of a combination of severity of deformity, complexity of deformity, and frequent association with patient obesity. We present here the outcome of treatment by osteotomy and gradual deformity correction by circular external fixation in 31 patients with either infantile or adolescent Blount's disease. We used a unique classification scheme to quantify and qualify complications in this patient group: category I, complications not requiring an alteration in the treatment plan, not involving unplanned return to surgery, and not influencing outcome; category II, complications requiring an alteration in the treatment plan, including unplanned returns to surgery, but that did not influence outcome; category IIIA, complications that resulted in a failure to achieve treatment goals; and category IIIB, complications that resulted in a failure to achieve treatment goals and the development of a new pathology or worsening of patient condition.

Deformity correction in pediatric skeletal dysplasia: treatment challenges and solutions.

We reviewed our experience with limb-deformity correction in 54 children with skeletal dysplasias. Our goal was to outline common treatment strategies developed in our hospital to overcome the challenges associated with the management of these conditions. Utilization of computer-assisted preoperative planning, intraoperative monitoring of peripheral nerve function, individualized bone segment stabilization using a modular circular external fixation system, and a flexible distraction protocol improved the precision of angular deformity correction in our practice, simplified external fixator assembly, diminished postoperative frame modifications, enhanced the stability of fixation, and reduced the rate of complications.

Treatment of femoral lengthening-related knee stiffness with a novel quadricepsplasty.

Background: Knee stiffness is common after femoral lengthening. Certain patients require a quadricepsplasty when therapy does not improve flexion. A small subset of such patients may also have obligate patellar dislocation with knee flexion due to contracture of the extensor mechanism.

Somatosensory evoked potential monitoring of peripheral nerves during external fixation for limb lengthening and correction of deformity in children.

We undertook a retrospective analysis of 306 procedures on 233 patients, with a mean age of 12 years (1 to 21), in order to evaluate the use of somatosensory evoked potential (SSEP) monitoring for the early detection of nerve compromise during external fixation procedures for limb lengthening and correction of deformity. Significant SSEP changes were identified during 58 procedures (19%). In 32 instances (10.