In search of molecular approaches to improving cancer therapy efficacy.

The study of genome rearrangement sites using full genome sequences is an important approach to revealing the nature of cancer and finding effective ways for cancer treatment. The progress in DNA sequencing could make the procedure of whole genome reading close to routine procedure of lower cost. The personal analysis of rearranged ends (PARE) method used for rearrangement study is reviewed.

[Recombination in Escherichia coli cells and multiform cytotoxic resistance].

Genetic crosses between E. coli Hfr C--donor and E. coli AB 1157--recipient cells sensitive to single target inhibitor glyphosate gave rise to offspring which could tolerate not only minimal inhibitory concentration of this inhibitor but also six-fold increased concentrations.

[Prions: on the enzymatic function of PrPc from mammalian and prion "families"].

This minireview deals with some approaches and results of experiments which enabled to discover SOD-like activity of the mammal PrP protein. This activity required the unchanged region of the repeated octapeptide and Cu2+ binding to the appropriate sites of the PrP molecule. It was shown that an infectious prion isoform could bind the normal isoform.

Uptake of the herbicidal glyphosate by Escherichia coli K-12.

The uptake of the aminoacid biosynthesis inhibitor, used as the broad-spectrum herbicide ingredient, glyphosate (N-[phosphonomethyl]-glycine) was investigated in E. coli as a model to study mechanisms of cell resistance to antimetabolites as drugs and pesticides. Unlike the glyphosate-degrading Arthrobacter sp.

[Characteristics of nuclear DNA of a Lepidoptera, the great wax moth Galleria mellonella, using reassociation kinetics].

Reassociation kinetics of great wax moth--Galleria mellonella nuclear DNA short fragments was studied within the C0t values of 0.02 and 10(4). The least squares analysis of the experimental curve obtained revealed two components with C0t1/2 = 0.

[Physico-chemical characteristics of the DNA of the nuclear polyhedrosis virus of the moth Porthetria dispar L].

DNA preparations were obtained after dissolving the inclusion bodies, polyhedra virus particles, from the purified bundle virus of Porthetria dispar L. nuclear polyhedrosis. The DNA molecules in the preparations obtained are of different conformation and separate within the CsCl density gradient in the presence of ethidium bromide into supercoiled catenated and relaxed circular molecules (with the admixture of linear molecules).

[A non-damaging procedure for DNA isolation from insect cell nuclei].

A gentle procedure is described for DNA isolation from Galleria mellonella cell nuclei. Nuclei lysis was performed by SDS in final concentration of 3% which was followed by DNA extraction from chromatin by a stepwise rise of NaCl concentration up to 2.5 M.

[Heterologous transformation: search for an explantation of the high effectiveness of Bacillus subtilis transformation achieved by using Bacillus natto DNA].

A higher efficiency of B. subtilis cells transformation by B. natto DNA is true for every concentration of DNA and is not due to the helping effect known for streptococci, strain Challis.