From Womb to World: Exploring the Immunological Connections between Mother and Child.

Mother and child are immunologically interconnected by mechanisms that we are only beginning to understand. During pregnancy, multiple molecular and cellular factors of maternal origin are transferred across the placenta and influence the development and function of the fetal and newborn immune system. Altered maternal immune states arising from pregnancy-associated infections or immunizations have the potential to program offspring immune function in ways that may have long-term health consequences.

Barrier crossing in a viscoelastic medium under active noise: Predictions of Kramers' flux-over-population method.

The biochemical activity inside a cell has recently been suggested to act as a source of hydrodynamic fluctuations that can speed up or slow down enzyme catalysis [Tripathi et al., Commun. Phys.

Internal friction as a factor in the anomalous chain length dependence of DNA transcriptional dynamics.

Recent experiments by Brückner et al. [Science 380, 1357 (2023)] have observed an anomalous chain length dependence of the time of near approach of widely separated pairs of genomic elements on transcriptionally active chromosomal DNA. In this paper, I suggest that the anomaly may have its roots in internal friction between neighboring segments on the DNA backbone.

Survival probabilities and first-passage distributions of self-propelled particles in spherical cavities.

A model of self-propelled motion in a closed compartment containing simple or complex fluids is formulated in this paper in terms of the dynamics of a point particle moving in a spherical cavity under the action of random thermal forces and exponentially correlated noise. The particle's time evolution is governed by a generalized Langevin equation (GLE) in which the memory function, connected to the thermal forces by a fluctuation-dissipation relation, is described by Jeffrey's model of viscoelasticity (which reduces to a model of ordinary viscous dynamics in a suitable limit). The GLE is transformed exactly to a Fokker-Planck equation that in spherical polar coordinates is in turn found to admit of an exact solution for the particle's probability density function under absorbing boundary conditions at the surface of the sphere.

The rpoS gene confers resistance to low osmolarity conditions in Salmonella enterica serovar Typhi.

Salmonella enterica serovars Typhimurium and Typhi are enteropathogens that differ in host range and the diseases that they cause. We found that exposure to a combination of hypotonicity and the detergent Triton X-100 significantly reduced the viability of the S. Typhi strain Ty2 but had no effect on the S.

Effects of Hydrodynamic Backflow on the Transmission Coefficient of a Barrier-Crossing Brownian Particle.

The slow power law decay of the velocity autocorrelation function of a particle moving stochastically in a condensed-phase fluid is widely attributed to the momentum that fluid molecules displaced by the particle transfer back to it during the course of its motion. The forces created by this backflow effect are known as Basset forces, and they have been found in recent analytical work and numerical simulations to be implicated in a number of interesting dynamical phenomena, including boosted particle mobility in tilted washboard potentials. Motivated by these findings, the present paper is an investigation of the role of backflow in thermally activated barrier crossing, the governing process in essentially all condensed-phase chemical reactions.

Statistical Dynamics of Flow-Driven Globular Polymers.

The response of collapsed polymers to the effects of linear mixed flow is studied theoretically in this paper using a model of a self-interacting finitely extensible Gaussian chain that evolves stochastically in the presence of random thermal fluctuations and an external fluid velocity gradient. The interactions that produce compact chain configurations are described by a harmonic pair potential of strength κ that acts between nonbonded sites on the chain backbone. Several chain properties are calculated analytically from this model as a function of κ for elongational and shear flows, including the dependence of the chain's steady-state mean-square end-to-end distance on the Weissenberg number of the flow, the time-dependence of the chain's relaxation to equilibrium from a steady-state of given chain extension, and the nature of the force-extension curves that are obtained from the free energy change between unperturbed and flow-stretched states of the chain.

RXRα Regulates the Development of Resident Tissue Macrophages.

Resident tissue macrophages (RTMs) develop from distinct waves of embryonic progenitor cells that seed tissues before birth. Tissue-specific signals drive a differentiation program that leads to the functional specialization of RTM subsets. Genetic programs that regulate the development of RTMs are incompletely understood, as are the mechanisms that enable their maintenance in adulthood.

Particle dynamics in viscoelastic media: Effects of non-thermal white noise on barrier crossing rates.

The growing interest in the dynamics of self-driven particle motion has brought increased attention to the effects of non-thermal noise on condensed phase diffusion. Thanks to data recently collected by Ferrer et al. on activated dynamics in the presence of memory [Phys.

The relaxation dynamics of single flow-stretched polymers in semidilute to concentrated solutions.

Recent experiments on the return to equilibrium of solutions of entangled polymers stretched by extensional flows [Zhou and Schroeder, Phys. Rev. Lett.

Helminth-Induced and Th2-Dependent Alterations of the Gut Microbiota Attenuate Obesity Caused by High-Fat Diet.

Background & Aims: Epidemiological and animal studies have indicated an inverse correlation between the rising prevalence of obesity and metabolic syndrome and exposure to helminths. Whether helminth-induced immune response contributes to microbiota remodeling in obesity remains unknown. The aim of this study is to explore the immune-regulatory role of helminth in the prevention of HFD-induced obesity through remodeling gut microbiome.

Spheres of Influence: Insights into Pathogenesis from Intestinal Organoids.

The molecular complexity of host-pathogen interactions remains poorly understood in many infectious diseases, particularly in humans due to the limited availability of reliable and specific experimental models. To bridge the gap between classical two-dimensional culture systems, which often involve transformed cell lines that may not have all the physiologic properties of primary cells, and in vivo animal studies, researchers have developed the organoid model system. Organoids are complex three-dimensional structures that are generated in vitro from primary cells and can recapitulate key in vivo properties of an organ such as structural organization, multicellularity, and function.

Stochastic thermodynamics of a harmonically trapped colloid in linear mixed flow.

In this paper, motivated by a general interest in the stochastic thermodynamics of small systems, we derive an exact expression-via path integrals-for the conditional probability density of a two-dimensional harmonically confined Brownian particle acted on by linear mixed flow. This expression is a generalization of the expression derived earlier by Foister and Van De Ven [J. Fluid Mech.

The YrbE phospholipid transporter of serovar Typhi regulates the expression of flagellin and influences motility, adhesion and induction of epithelial inflammatory responses.

serovar Typhi is the etiologic agent of typhoid fever, a major public health problem in the developing world. Moving toward and adhering to the intestinal epithelium represents key initial steps of infection by . Typhi.

The commensal bacterium Bacteroides fragilis down-regulates ferroportin expression and alters iron homeostasis in macrophages.

The intestinal microbiota has several effects on host physiology. Previous work from our laboratory demonstrated that the microbiota influences systemic iron homeostasis in mouse colitis models by altering inflammation-induced expression of the iron-regulating hormone hepcidin. In the present study, we examined the impact of the gut commensal bacterium Bacteroides fragilis on the expression of the iron exporter ferroportin, the target of hepcidin action, in macrophages, the cell type that plays a pivotal role in iron recycling.

Serum siderocalin levels in patients with tuberculosis and HIV infection.

Objectives: Mycobacterium tuberculosis produces high-affinity siderophores that play essential roles in iron acquisition and tuberculosis (TB) pathogenesis. In response, host cells secrete a siderophore-binding protein, siderocalin, to limit the bacteria's access to iron. The objective of the present study was to evaluate the levels of siderocalin in patients with TB with or without HIV infection compared to controls.

The effects of slit-like confinement on flow-induced polymer deformation.

This paper is broadly concerned with the dynamics of a polymer confined to a rectangular slit of width D and deformed by a planar elongational flow of strength γ̇. It is interested, more specifically, in the nature of the coil-stretch transition that such polymers undergo when the flow strength γ̇ is varied, and in the degree to which this transition is affected by the presence of restrictive boundaries. These issues are explored within the framework of a finitely extensible Rouse model that includes pre-averaged surface-mediated hydrodynamic interactions.

Non-Gaussian Brownian Diffusion in Dynamically Disordered Thermal Environments.

In this article, we suggest simple alternatives to the methods recently used by Jain and Sebastian [ J. Phys. Chem.

Helminth-induced alterations of the gut microbiota exacerbate bacterial colitis.

Infection with the intestinal helminth parasite Heligmosomoides polygyrus exacerbates the colitis caused by the bacterial enteropathogen Citrobacter rodentium. To clarify the underlying mechanism, we analyzed fecal microbiota composition of control and helminth-infected mice and evaluated the functional role of compositional differences by microbiota transplantation experiments. Our results showed that infection of Balb/c mice with H.

Iron and inflammation - the gut reaction.

Anemia is a frequent complication of many inflammatory disorders, including inflammatory bowel disease. Although the pathogenesis of this problem is multifactorial, a key component is the abnormal elevation of the hormone hepcidin, the central regulator of systemic iron homeostasis. Investigations over the last decade have resulted in important insights into the role of hepcidin in iron metabolism and the mechanisms that lead to hepcidin dysregulation in the context of inflammation.

Polymer extension under flow: Some statistical properties of the work distribution function.

In an extension of earlier studies from this group on the application of the Jarzynski equality to the determination of the elastic properties of a finitely extensible Rouse model of polymers under flow [A. Ghosal and B. J.

Antibiotic Treatment Induces Long-lasting Changes in the Fecal Microbiota that Protect Against Colitis.

Background: The interplay between host genetics, immunity, and microbiota is central to the pathogenesis of inflammatory bowel disease. Previous population-based studies suggested a link between antibiotic use and increased inflammatory bowel disease risk, but the mechanisms are unknown. The purpose of this study was to determine the long-term effects of antibiotic administration on microbiota composition, innate immunity, and susceptibility to colitis, as well as the mechanism by which antibiotics alter host colitogenicity.

Commensal Bacteria-Induced Inflammasome Activation in Mouse and Human Macrophages Is Dependent on Potassium Efflux but Does Not Require Phagocytosis or Bacterial Viability.

Gut commensal bacteria contribute to the pathogenesis of inflammatory bowel disease, in part by activating the inflammasome and inducing secretion of interleukin-1ß (IL-1ß). Although much has been learned about inflammasome activation by bacterial pathogens, little is known about how commensals carry out this process. Accordingly, we investigated the mechanism of inflammasome activation by representative commensal bacteria, the Gram-positive Bifidobacterium longum subspecies infantis and the Gram-negative Bacteroides fragilis.