Publications by authors named "Cheon-Seok Park"

Article Synopsis
  • Amylosucrase (ASase) from Deinococcus geothermalis (DgAS) is characterized as a dimeric enzyme that produces α-1,4-glucans using sucrose, and this study reveals key amino acids important for maintaining its dimeric structure.
  • The mutated monomeric form (DgAS R30A) shows a stronger affinity for sucrose and preferentially produces shorter α-glucans with a degree of polymerization (DP) of ≤20.
  • The research also uncovers the first high-resolution structure of dimeric DgAS, providing insights into enzyme activity and the significance of dimerization for its functional properties.
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is a traditional Korean soybean brick characterized by diverse microbial communities. The microbial communities in were identified at the phylum and genus levels using high-throughput sequencing. During fermentation, diverse factors such as total bacterial cell numbers, moisture content, salinity, pH, enzyme activities, and free amino acids were monitored.

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Here, we investigated the complexation of short chain amylose (SCAs) and palmitic acid (PA), serving as polymeric building blocks that alter the selectivity and directionality of particle growth. This alteration affects the shape anisotropy of the particles, broadening their applications due to the increased surface area. By modifying the concentration of PA, we were able to make spherical, macaron, and disc-shaped particles, demonstrating that PA acts as a structure-directing agent.

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Intricate ecosystem of the human gut microbiome is affected by various environmental factors, genetic makeup of the individual, and diet. Specifically, resistant starch (RS) is indigestible in the small intestine but nourishes the gut microbiota in the colon. Degradation of RS in the gut begins with primary degraders, such as and .

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The fecal microbiota of two healthy adults was cultivated in a medium containing commercial fructooligosaccharides [FOS; 1-kestose (GF), nystose (GF), and 1-fructofuranosylnystose (GF)]. Initially, the proportions of lactobacilli in the two feces samples were only 0.42% and 0.

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Amylosucrase (ASase) efficiently biosynthesizes α-glucoside using flavonoids as acceptor molecules and sucrose as a donor molecule. Here, ASase from Deinococcus wulumuqiensis (DwAS) biosynthesized more naringenin α-glucoside (NαG) with sucrose and naringenin as donor and acceptor molecules, respectively, than other ASases from Deinococcus sp. The biotransformation rate of DwAS to NαG was 21.

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α-Glucan microparticles (GMPs) have significant potential as high-value biomaterials in various industries. This study proposes a bottom-up approach for producing GMPs using four amylosucrases from sp. (BASs).

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Unlabelled: Recently, unconventional yeasts have become popular as fermentation starters in the brewing industry due to the growing consumer demand for aromatic diversity. Specifically, has been explored as a potential starter culture for beer and wine production because of its distinct brewing characteristics; however, its application in fermentation has not been tested. Therefore, in the present study, two strains (SZJ-1 and SZJ-2) were isolated from natural sources, and their brewing characteristics for fermentation were compared with those of commercial strain.

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Resistant starch (RS) reaches the large intestine largely intact, where it is fermented by the gut microbiota, resulting in the production of short-chain fatty acids (SCFAs) that have beneficial effects on the human body. Bifidobacteria are a major species widely used in the probiotic field, and are increased in the gut by RS, indicating their importance in RS metabolism in the intestine. Bifidobacteria have a genetic advantage in starch metabolism as they possess a significant number of starch-degrading enzymes and extraordinary three RS-degrading enzymes, allowing them to utilize RS.

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Soybean isoflavone aglycones (SIAs) have many biological activities but are poorly water-soluble in the human body. Glycosylation provides structural diversity to SIAs and can alter their physicochemical properties, including water solubility. An alpha-linked glucosylation of SIA was achieved using amylosucrase from .

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The 4-α-glucanotransferase (4-α-GTase or amylomaltase) is an essential enzyme in maltodextrin metabolism. Generally, most bacterial 4-α-GTase is classified into glycoside hydrolase (GH) family 77. However, hyperthermophiles have unique 4-α-GTases belonging to GH family 57.

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Unlabelled: Amylosucrase from (AS) synthesizes α-1,4 glucan polymer from sucrose. In this study, we coated various botanical sources of raw starch with an α-glucan layer generated by AS to improve physicochemical properties. Field-emission scanning electron microscopy demonstrated that all surfaces of the starch granules were successfully coated via the AS reaction.

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Unlabelled: Resistant starch (RS) in the diet reaches the large intestine and is fermented by the gut microbiota, providing beneficial effects on human health. The human gut bacterium FMB-CY1 was isolated and identified as a new species closest to . sp.

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A putative type II pullulanase gene, pulP, was identified in Bifidobacterium adolescentis P2P3. PulP possesses an α-amylase domain at the N-terminus and a pullulanase type I domain at the C-terminus, as well as three carbohydrate-binding modules (one CBM25 and two CBM41s) between them. The native PulP and four truncated mutant recombinant proteins (PulPΔCΔP, PulPΔP, PulPΔAΔC, and PulPΔA), in which each of the two catalytic domains and/or the CBMs were deleted, were produced in Escherichia coli and their specific properties were characterized.

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Diverse flavonoid glycosides are present in the plant kingdom. Advanced technologies have been utilized to synthesize glycosyl flavonoids which exhibit good physicochemical characteristics. Previously, novel isoquercitrin (IQ) mono-, di-, and tri-glucosides (IQ-G1', IQ-G2', and IQ-G3'; atypical IQ-Gs (IQ-G)) were synthesized through the reaction of amylosucrase.

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Eco-friendly conductive polymer nanocomposites have garnered attention as an effective alternative for conventional conductive nanocomposites. Here, we report the fabrication and optimization of flexible, self-standing, and conductive bacterial cellulose/poly(3,4-ethylene dioxythiophene) (BC/PEDOT) nanocomposites using the vapor phase polymerization (VPP) method. Eco-friendly bacterial cellulose (BC) is used as a flexible matrix, and the highly conductive PEDOT polymer is introduced into the BC matrix to achieve electronic conductivity.

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Many attempts have been made to obtain natural products with certain glycosidic linkages for improvement of their chemo-physical characteristics. Amylosucrase from (DGAS; EC.4.

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The coffee diterpene kahweol may contribute to the anti-obesity effects of coffee but its physiological effects have yet to be elucidated. is used as an animal model in obesity research because its lipid metabolism is conserved in humans. The goal was to investigate kahweol's effects on lipid metabolism in .

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Butein, a flavonoid found in annatto seeds and lacquer trees, may be used for many health benefits, including the prevention of obesity. However, its anti-obesity effects are not completely understood; in particular, the effects of butein on the regulation of lipid metabolism have not been explained. Thus, the goal of the current study was to determine the effects of butein on lipid metabolism in Caenorhabditis elegans, which is a multi-organ nematode used as an animal model in obesity research.

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