Development of a self-assembled dual-enzyme co-display platform on the surface of the natural "chitosan beads" of yeast spores.

Under starvation conditions, Saccharomyces cerevisiae diploid cells initiate meiosis to produce dormant cells called spores. When the DIT1 gene involved in assembling the outermost layer dityrosine is disrupted, the natural "chitosan beads" of yeast spores will be formed. A novel cell surface display system based on "chitosan beads" of dit1Δ yeast spores was previously established.