Characteristics of Pore Structure and the Evolution Process in Terrestrial Shale Reservoirs: A Case Study of the Shahejie Formation in Dongpu Depression, Bohai Bay Basin.

Microscopic pore characteristics of shale are very important for exploring the oil occurrence and content. However, previous studies on heterogeneous terrestrial shale are lacking. We choose the Shahejie formation (SF) of the Dongpu Depression in the Bohai Bay Basin as a case study.

Molecular Triplet Generation Enabled by Adjacent Metal Nanoparticles.

High-efficiency generation of spin-triplet states in organic molecules is of great interest in diverse areas such as photocatalysis, photodynamic therapy, and upconversion photonics. Recent studies have introduced colloidal semiconductor nanocrystals as a new class of photosensitizers that can efficiently transfer their photoexcitation energy to molecular triplets. Here, we demonstrate that metallic Ag nanoparticles can also assist in the generation of molecular triplets in polycyclic aromatic hydrocarbons (PAHs), but not through a conventional sensitization mechanism.

The role of JMJD2A in immune evasion and malignant behavior of esophageal squamous cell carcinoma.

Objective: This study aimed to investigate the role of JMJD2A in radiotherapy tolerance of esophageal squamous cell carcinoma (ESCC).

Methods: The levels of H3K9me3 modification were analyzed in anti-PD-1 therapy non-responder or responder patients, and the expression differences of H3K9me3-related modifying enzymes were assessed in TCGA-ESCC and ICGC cohorts. Subsequently, JMJD2A was knocked down in ESCC cells using CRISPR-Cas9 or lentivirus-mediated shRNA, and changes in malignant behavior of ESCC cells were observed.

Development of the Colorimetric and/or Fluorescent Probes for Detecting Fluoride ions in Aqueous Solution.

Fluoride ion is a strong Lewis base and one of the essential trace elements in human body. It plays a very important role in human health and ecological balance. The deficiency or excessive intake of fluoride ions will cause serious health problems, so the development of a sensitive and accurate detection method for fluoride ions is very important.

Prolactin and glucocorticoid receptors in the prefrontal cortex are associated with anxiety-like behavior in prenatally stressed adolescent offspring rats.

Prenatal stress (PS) causes anxiety in mothers and their offspring and chewing is a commonly observed behavior during maternal stress. Prolactin (PRL) is an anti-anxiety factor that suppresses the hypothalamic-pituitary-adrenal axis. Here, we studied the roles of PRL, corticosterone (CORT), and their receptors in PS-induced anxiety-like behavior in dams and their offspring.

A Novel Detachable, Reusable, and Versatile Acoustic Tweezer Manipulation Platform for Biochemical Analysis and Detection Systems.

Multifunctional, integrated, and reusable operating platforms are highly sought after in biochemical analysis and detection systems. In this study, we demonstrated a novel detachable, reusable acoustic tweezer manipulation platform that is flexible and versatile. The free interchangeability of different detachable microchannel devices on the acoustic tweezer platform was achieved by adding a waveguide layer (glass) and a coupling layer (polydimethylsiloxane (PDMS) polymer film).

Associations between maternal prenatal depression and neonatal behavior and brain function - Evidence from the functional near-infrared spectroscopy.

Background: Maternal prenatal depression is a significant public health issue associated with mental disorders of offspring. This study aimed to determine if maternal prenatal depressive symptoms are associated with changes in neonatal behaviors and brain function at the resting state.

Methods: A total of 204 pregnant women were recruited during the third trimester and were evaluated by Edinburgh Postpartum Depression Scale (EPDS).

A lncRNA fine-tunes salicylic acid biosynthesis to balance plant immunity and growth.

Constitutive activation of plant immunity is detrimental to plant growth and development. Here, we uncover the role of a long non-coding RNA (lncRNA) in fine-tuning the balance of plant immunity and growth. We find that a lncRNA termed salicylic acid biogenesis controller 1 (SABC1) suppresses immunity and promotes growth in healthy plants.

Fault Detection in the MSW Incineration Process Using Stochastic Configuration Networks and Case-Based Reasoning.

Fault detection in the waste incineration process depends on high-temperature image observation and the experience of field maintenance personnel, which is inefficient and can easily cause misjudgment of the fault. In this paper, a fault detection method is proposed by combining stochastic configuration networks (SCNs) and case-based reasoning (CBR). First, a learning pseudo metric method based on SCNs (SCN-LPM) is proposed by training SCN learning models using a training sample set and defined pseudo-metric criteria.

Effect of deformation of a flat plate specimen under in-plane blasting loading on 2D and 3D digital image correlation measurement.

In combination with high-speed photography, a 3D digital image correlation (DIC) experimental system suitable for an in-plane blasting experiment is established to effectively explore the mechanism of blasting. The dynamic response of the specimen under in-plane blasting loading is analyzed on the basis of the model experiment, and the effect of out-of-plane deformation on the 2D-DIC measurement under a blasting stress wave is studied. First, the out-of-plane displacement and strain evolution law of the specimen under blasting load are studied by using a 3D-DIC system.

Development and Evaluation of Two Double-Antibody Sandwich ELISAs for Detecting Severe Fever with Thrombocytopenia Syndrome Virus Infection.

Severe fever with thrombocytopenia syndrome virus (SFTSV) is a newly emerging tick-borne virus with a fatality rate between 12% and 50%. Currently, effective vaccines or antiviral drugs are not available to treat SFTSV infection, and a diagnostic method for its detecting is urgently needed. Monoclonal (MAb) and polyclonal antibodies (PAbs) against SFTSV were prepared by immunizing animals with the SFTSV nucleocapsid protein (NP).

Experimental study on the effect of defect curvature on the impact fracture behavior of structures using the real-virtual caustics method.

Defects have significant influence on the impact fracture behavior of structures. In this paper, the real-virtual caustics method is used to study the impact fracture behavior of structures with elliptical arc defects under impact loading of the drop hammer, and the impact loading process is simultaneously analyzed. The research results show that impact loading of the drop hammer in this experiment is a multi-period dynamic loading process, and the fracture of specimens under impact loading of the drop hammer is an energy-controlled process.

Novel CD123 polyaptamer hydrogel edited by Cas9/sgRNA for AML-targeted therapy.

CD123 targeting molecules have been widely applied in acute myelocytic leukemia (AML) therapeutics. Although antibodies have been more widely used as targeting molecules, aptamer have unique advantages for CD123 targeting therapy. In this study, we constructed an aptamer hydrogel termed as SSFH which could be precisely cut by Cas9/sgRNA for programmed SS30 release.

A Novel pH-Sensitive Multifunctional DNA Nanomedicine: An Enhanced and Harmless GD2 Aptamer-Mediated Strategy for Guiding Neuroblastoma Antitumor Therapy.

Background: GD2 is a mainstream biomarker for neuroblastoma (NB)-targeted therapy. Current anti-GD2 therapeutics exhibit several side effects since GD2 is also expressed at low levels on normal cells. Thus, current anti-GD2 therapeutics can be compromised by the coexistence of the target receptor on both cancer cells and normal cells.

Design and application of a comprehensive experimental system for real imaging-virtual imaging of dynamic caustics.

On the basis of the principle of caustics, a comprehensive experimental system for real imaging-virtual imaging was designed and established and then applied to dynamic fracture mechanics experiments. An explosion load experimental system was used to investigate the interaction between blast stress waves and prefabricated cracks. The virtual image information of the caustic spot of the crack tip under the radial compressive stress of the explosive load was captured and quantified.

Distribution and genetic diversity of adeno-associated viruses in bats from coastal areas of Southeast China.

Bats are associated with several important zoonotic viruses from different families. One example includes adeno-associated viruses (AAVs), that are extensively detected in several animals, especially primates. To understand AAVs distribution and genetic diversity in the coastal areas of Southeast China, a total of 415 intestine samples were mostly collected from two provinces of southeast China, i.

A novel small RNA contributes to restrain cellular chain length and anti-phagocytic ability in Streptococcus suis 2.

Streptococcus suis serotype 2 (SS2) is an important porcine and human pathogen. Regulatory small non-coding RNAs (sRNAs) play an essential role in diverse physiological processes, although they remain poorly understood in SS2. In this study, we identified eight novel sRNAs through a combination of computational strategies and experimental identification.

Virome analysis for identification of novel mammalian viruses in bats from Southeast China.

Bats have been shown as important mammal resevoirs to carry a variety of zoonotic pathogens. To analyze pathogenic species in bats from southeast coastal regions of China, we performed metagenomic sequencing technology for high throughput sequencing of six sentinels from southeast coastal area of China. We obtained 5,990,261 high quality reads from intestine and lung tissue of 235 bats, including 2,975,371 assembled sequences.

One Step to Separate Five Alkaloids from Hypecoum leptocarpum by High-Speed Counter-Current Chromatography.

High-speed counter-current chromatography (CCC) was firstly and successfully applied for the preparative separation and purification of alkaloids from crude extract of Hypecoum leptocarpum. After the measurement of partition coefficient of five target alkaloids in the two-phase solvent systems, the CCC was performed well with a two-phase solvent system composed of tetrachloromethane-chloroform-methanol-0.1 M HCl at a volume ratio of 1.

In vitro and in vivo biological activities of anthocyanins from Nitraria tangutorun Bobr. fruits.

Anthocyanins are the main compounds in Nitraria tangutorun Bobr. The enrichment and purification of anthocyanins on macroporous resins were investigated. Regarding anthocyanin purification, static adsorption and desorption were studied.