Immobilizing c(RGDfc) on the surface of metal-phenolic networks by thiol-click reaction for accelerating osteointegration of implant.

The limited osteointegration often leads to the failure of implant, which can be improved by fixing bioactive molecules onto the surface, such as arginyl-glycyl-aspartic acid (RGD): a cell adhesion motif. Metal-Phenolic Networks (MPNs) have garnered increasing attention from different disciplines in recent years due to their simple and rapid process for depositing on various substrates or particles with different shapes. However, the lack of cellular binding sites on MPNs greatly blocks its application in tissue engineering.

Engineering tunable dual peptide hybrid coatings promote osseointegration of implants.

Utilizing complementary bioactive peptides is a promising surface engineering strategy for bone regeneration on osteogenesis. In this study, we designed block peptides, (Lysine)-capped RGD (K-(linker-RGD)) and OGP (K-linker-(YGFGG)), which were mildly grafted onto PC/Fe-MPNs through supramolecular interactions between K and PC residues on the MPNs surface to form a dual peptide coating, named PC/Fe@K-RGD/OGP. The properties of the block peptides coating, including mechanics, hydrophilicity, chemical composition, etc.

Using deep learning for the automated identification of cone and rod photoreceptors from adaptive optics imaging of the human retina.

Adaptive optics imaging has enabled the enhanced in vivo retinal visualization of individual cone and rod photoreceptors. Effective analysis of such high-resolution, feature rich images requires automated, robust algorithms. This paper describes RC-UPerNet, a novel deep learning algorithm, for identifying both types of photoreceptors, and was evaluated on images from central and peripheral retina extending out to 30° from the fovea in the nasal and temporal directions.

A Dual-Mode Optical Thermometer with High Sensitivity Based on BaAlO:Sm/SrAlO:Sm Solid Solution Phosphors.

A series of BaAlO:Sm/SrAlO:Sm mixed-phase phosphors were produced in one step using the traditional high-temperature solid-phase process. Because Sm is divalent in BaAlO and trivalent in SrAlO, the coexistence of Sm and Sm is realized in the mixed-phase host. Since the temperature sensitivity of Sm and Sm in the solid solution host is significantly different, this makes it possible for the sample to measure temperature based on the fluorescence intensity ratio (FIR).

Myricetin induces apoptosis in HepG2 cells through Akt/p70S6K/bad signaling and mitochondrial apoptotic pathway.

The present investigation was undertaken to gain insight into the molecular mechanism by which myricetin induces apoptosis in human hepatocarcinoma HepG2 cells. Myricetin caused the disruption of mitochondrial membrane potential in a dose-dependent manner. Moreover, myricetin triggered translocation of the pro-apoptotic protein Bax to the mitochondria, downregulation of anti-apoptotic Bcl-2 expression and upregulated the expression of pro-apoptotic protein Bad in the mitochondria.

Myricetin induces G2/M phase arrest in HepG2 cells by inhibiting the activity of the cyclin B/Cdc2 complex.

Myricetin, a naturally occurring flavonol, has been shown to inhibit the proliferation of human hepatoma HepG2 cells and to induce G2/M phase arrest. However, the underlying mechanisms of Myricetin activity have yet to be revealed. The aim of the present study was to clarify the molecular mechanisms of cell cycle arrest induced by myricetin in HepG2 cells.

[Effect of pregnancy rats supplemented with docosahexaenoic acid on carnitine palmitoyl transferase-I gene expression in offspring].

Objective: To investigate the effect on lipid metabolic and carnitine palmitoyl transferase-I (CPT-I) gene expression of offspring when their maternal rats supplemented with docosahexaenoic acid (DHA) during pregnancy.

Method: Female SD rats were randomly divided into A, B, C groups. Group A was fed with basic diet, group B was supplemented with low dose of DHA, and group C supplemented with high dose of DHA during pregnancy.