Publications by authors named "Chenwei Lin"

Luminescent responsive heterostructures with region-domained emission and integrated responsiveness exhibit great potential in information security, but always suffer from the direct exposure of fingerprint information at the initial state, making it easy to decode the hidden confidential information. Herein, the first monochromatic responsive hydrogen-bonded organic framework (HOF) heterostructures are reported based on VIA-group-based framework hybridization toward fully-covert photonic barcodes. Designed HOF blocks with different VIA-group elements are integrated via a configuration-assimilation-based assembly method to generate the intrinsic monochromatic HOF heterostructures.

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Assessing antibody titers and functional responses is essential for evaluating vaccine efficacy, yet the impact of blood collection methods on these immunological assessments remains unclear. Retro-orbital (RO) blood collection is commonly used but significant complications can occur. Increasingly, investigators have adopted alternative blood collection approaches, such as saphenous vein (SV) sampling to improve laboratory animal welfare.

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The maternal-to-zygotic transition (MZT) is a conserved developmental process where the maternally-derived protein and mRNA cache is replaced with newly made zygotic gene products. We have previously shown that in Drosophila the deposited RNA-binding proteins ME31B, Cup, and Trailer Hitch are ubiquitylated by the CTLH E3 ligase and cleared. However, the organization and regulation of the CTLH complex remain poorly understood in flies because Drosophila lacks an identifiable substrate adaptor, and the mechanisms restricting the degradation of ME31B and its cofactors to the MZT are unknown.

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Introduction: Acute myeloid leukemia (AML) remains one of the deadliest hematopoietic malignancies. A better understanding of the molecular biology governing AML may lead to improved risk stratification and facilitate the development of novel therapies. Proteins are responsible for much of the biology of cells.

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Objective: This study aimed to explore whether excessive HIF2α can amplify the impact of human Umbilical Cord Mesenchymal Stem Cell-derived Extracellular Vesicles (hUC-MSC- EVs) on endothelial cells.

Methods: In this study, we created HIF2α-overexpressing hUC-MSC-EVs and compared their pro-angiogenic effects with control EVs on Human Umbilical Vein Endothelial Cells (HUVECs). MTT assay and Edu staining were used to detect the viability and proliferation ability of HUVECs, and Transwell and tube formation assays were used to detect cell migration and tube formation ability.

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Metal-organic framework (MOF) heterostructures with hybrid architectures and abundant functional sites possess great potential applications in advanced information security, yet still suffer from the harsh stimuli mechanisms with restrained emission control. Herein, the differentiated design strategy on intra-ligand charge transfer is first reported to realize smart-responsive multicolor MOF heterostructures as robust anticounterfeiting labels. Designed similar MOF blocks with the differentiated intra-ligand charge transfer are integrated via time-dependent epitaxial growth to form multicolor MOF heterostructures.

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The maternal-to-zygotic transition (MZT) is a conserved developmental process where the maternally-derived protein and mRNA cache is replaced with newly made zygotic gene products. We have previously shown that in the deposited RNA-binding proteins ME31B, Cup, and Trailer Hitch (TRAL) are ubiquitylated by the CTLH E3 ligase and cleared. However, the organization and regulation of the CTLH complex remain poorly understood in flies.

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Immunotherapies are revolutionizing cancer care, but many patients do not achieve durable responses and immune-related adverse events are difficult to predict. Quantifying the hundreds of proteins involved in cancer immunity has the potential to provide biomarkers to monitor and predict tumor response. We previously developed robust, multiplexed quantitative assays for immunomodulatory proteins using targeted mass spectrometry, providing measurements that can be performed reproducibly and harmonized across laboratories.

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This case discussed a significant ocular side effect, bilateral keratitis, which could be induced by afatinib, an irreversible epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI). We explored the disease progression of a 52-year-old, stage IV nasopharyngeal carcinoma male patient, who was under afatinib treatment and had experienced progressive bilateral eye dryness and tenderness on increasing afatinib from 40 mg every other day to 40 mg daily. Clinical examination noted bilateral visual acuity reduction, diffuse superficial punctate keratopathy in the right eye, and a central epithelial defect in the left eye.

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Article Synopsis
  • Cells use protein interactions to send biological signals.
  • The study identified 128 proteins that interacted more after engaging a specific CAR T cell receptor, showing differences in signaling compared to regular T cell receptors.
  • Variations in production of the IL-2 cytokine were linked to differences in protein network activation, suggesting that future CAR T cell manufacturing could benefit from monitoring these signaling changes.
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A wealth of proteogenomic data has been generated using cancer samples to deepen our understanding of the mechanisms of cancer and how biological networks are altered in association with somatic mutation of tumor suppressor genes, such as TP53 and PTEN. To generate functional signatures of TP53 or PTEN loss, we profiled the RNA and phosphoproteomes of the MCF10A epithelial cell line, along with its congenic TP53- or PTEN-knockout derivatives, upon perturbation with the monofunctional DNA alkylating agent methyl methanesulfonate (MMS) vs. mock treatment.

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  • * Results showed that measurements from the different tonometers were comparable, with strong correlations and good agreement, particularly between the Icare and Tono-Pen.
  • * The findings suggest that these alternative IOP measurement devices are reliable, potentially enhancing clinical practices in diagnosing and managing eye conditions.
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  • Researchers analyzed the proteogenomic profiles of 242 high-grade serous ovarian cancers (HGSOCs) to better understand those that are resistant to chemotherapy.
  • A specific 64-protein signature was identified that accurately predicts which HGSOCs are likely to be resistant to platinum-based treatments, confirming findings in multiple patient groups.
  • The study also found a link between the absence of Ch17 loss of heterozygosity and chemotherapy resistance, leading to the classification of HGSOCs into five clusters, each suggesting different resistance mechanisms and potential treatment targets.
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  • The increasing cases of diabetes due to an aging population strain healthcare systems and create public health issues, prompting the need for better monitoring methods.
  • A new nano-sensing probe that uses only saliva for measuring blood sugar levels shows promise, as it is less invasive and more stable than traditional blood tests.
  • The study found a significant correlation between salivary conductivity and diabetes risk, suggesting that this portable device could serve as an effective screening tool for early diabetes detection.
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Introduction: Immunotherapy is an effective treatment for a subset of cancer patients, and expanding the benefits of immunotherapy to all cancer patients will require predictive biomarkers of response and immune-related adverse events (irAEs). To support correlative studies in immunotherapy clinical trials, we are developing highly validated assays for quantifying immunomodulatory proteins in human biospecimens.

Methods: Here, we developed a panel of novel monoclonal antibodies and incorporated them into a novel, multiplexed, immuno-multiple reaction monitoring mass spectrometry (MRM-MS)-based proteomic assay targeting 49 proteotypic peptides representing 43 immunomodulatory proteins.

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  • - Sulfonylureas, often used with metformin in treating type 2 diabetes (T2D), show varying cardiovascular risks based on their affinity to cardiac mitoKATP channels, which hasn't been adequately studied in broader diabetic populations before.
  • - The main goal of the study was to analyze the risk of major cardiovascular events, such as myocardial infarction (MI), ischemic stroke, and cardiovascular death, in T2D patients using high-affinity vs low-affinity sulfonylureas combined with metformin.
  • - Results from over 53,000 participants indicated that patients on high-affinity sulfonylureas (like glyburide and glipizide) faced an increased risk of major adverse
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  • Scientists are interested in studying proteins in tissue samples to understand diseases and find new medical tests.
  • A new device was created that quickly freezes tissue without needing liquid nitrogen, which is sometimes hard to get in hospitals.
  • Tests showed that tissue samples frozen with this new device kept their important proteins just as well as those frozen with the traditional method using liquid nitrogen.
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  • Acute myeloid leukemia (AML) is a challenging cancer with few treatment options, and the study combines ex vivo drug sensitivity with genomic data from 805 patients to better understand treatment responses.
  • This research identifies key features influencing drug sensitivity, particularly focusing on the differentiation state of AML cells, which has implications for how they respond to therapy.
  • Notably, the gene PEAR1 emerges as a strong predictor of survival in young AML patients, highlighting its potential role in guiding treatment decisions and future drug development.
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Despite advances in proteomic technologies, clinical translation of plasma biomarkers remains low, partly due to a major bottleneck between the discovery of candidate biomarkers and costly clinical validation studies. Due to a dearth of multiplexable assays, generally only a few candidate biomarkers are tested, and the validation success rate is accordingly low. Previously, mass spectrometry-based approaches have been used to fill this gap but feature poor quantitative performance and were generally limited to hundreds of proteins.

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  • The prevalence of chronic kidney disease (CKD) is on the rise, causing significant healthcare challenges, primarily due to reliance on invasive blood tests that hinder early detection and awareness.
  • Researchers developed a portable device to measure salivary conductivity using just 50 μL of saliva, finding that CKD patients had notably higher salivary conductivity levels.
  • A study involving 214 adult participants indicates that elevated salivary conductivity is positively linked to age and fasting glucose but inversely related to kidney function; this suggests that salivary conductivity could be an effective non-invasive marker for early CKD screening.
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Objective: Previous studies have revealed an intraclass difference in major adverse cardiovascular events (MACE) among sulfonylureas. In vitro and ex vivo studies reported several sulfonylureas to exhibit high-affinity blockage of cardiac mitochondrial ATP-sensitive potassium (mitoKATP) channels and could interfere with ischemic preconditioning, the most important mechanism of self-cardiac protection. However, no studies have examined whether these varying binding affinities of sulfonylureas could account for their intraclass difference in MACE.

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Resistance to platinum compounds is a major determinant of patient survival in high-grade serous ovarian cancer (HGSOC). To understand mechanisms of platinum resistance and identify potential therapeutic targets in resistant HGSOC, we generated a data resource composed of dynamic (±carboplatin) protein, post-translational modification, and RNA sequencing (RNA-seq) profiles from intra-patient cell line pairs derived from 3 HGSOC patients before and after acquiring platinum resistance. These profiles reveal extensive responses to carboplatin that differ between sensitive and resistant cells.

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Immunotherapies are revolutionizing cancer care, producing durable responses and potentially cures in a subset of patients. However, response rates are low for most tumors, grade 3/4 toxicities are not uncommon, and our current understanding of tumor immunobiology is incomplete. While hundreds of immunomodulatory proteins in the tumor microenvironment shape the anti-tumor response, few of them can be reliably quantified.

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β-thalassemia is a hereditary hematological disease caused by over 350 mutations in the β-globin gene (HBB). Identifying the genetic variants affecting fetal hemoglobin (HbF) production combined with the α-globin genotype provides some prediction of disease severity for β-thalassemia. However, the generation of an additive composite genetic risk score predicts prognosis, and guide management requires a larger panel of genetic modifiers yet to be discovered.

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Synopsis of recent research by authors named "Chenwei Lin"

  • - Chenwei Lin's recent research emphasizes the development and application of advanced mass spectrometry techniques to understand and quantify immunomodulatory proteins, which can serve as potential biomarkers for cancer treatment outcomes and adverse events associated with immunotherapy.
  • - His work also explores the cellular mechanisms involved in chemotherapy resistance, particularly in high-grade serous ovarian cancer, by identifying specific protein signatures and molecular pathways linked to treatment failure, which could guide future therapeutic strategies.
  • - Additionally, Lin investigates the role of various signaling pathways and protein interactions in immune cell responses, including those mediated by chimeric antigen receptors, providing insights that may enhance the efficacy of cancer immunotherapies through better understanding of tumor-microenvironment interactions.

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