GRK2 Protein Mediates the ANRIL, a lncRNA, to Affect the Proliferation and Apoptosis of Kasumi-1 Cells.

Background: A long non-coding RNAs (LncRNAs) called antisense noncoding RNA in the INK4 locus (), has emerged as substantial regulators of cell survival in acute myeloid leukemia (AML). However, its speciffc and potential mechanism is uncertain in AML. In this research, we investigated the role of in cell proliferation, apoptosis, and the underlying mechanism in AML cells.

Robust Ellipse Fitting Based on Maximum Correntropy Criterion With Variable Center.

The presence of radically irregular data points (RIDPs), which are referred to as the subset of measurements that represents no or little information, can significantly degrade the performance of ellipse fitting methods. We develop an ellipse fitting method that is robust to RIDPs based on the maximum correntropy criterion with variable center (MCC-VC), where an adaptable Laplacian kernel is used. For single ellipse fitting, we formulate a non-convex optimization problem and divide it into two subproblems, one to estimate the kernel bandwidth and the other the kernel center.

mRNA based vaccines provide broad protection against different SARS-CoV-2 variants of concern.

In order to overcome the pandemic of COVID-19, messenger RNA (mRNA)-based vaccine has been extensively researched as a rapid and versatile strategy. Herein, we described the immunogenicity of mRNA-based vaccines for Beta and the most recent Omicron variants. The homologous mRNA-Beta and mRNA-Omicron and heterologous Ad5-nCoV plus mRNA vaccine exhibited high-level cross-reactive neutralization for Beta, original, Delta, and Omicron variants.

Robust Ellipse Fitting With Laplacian Kernel Based Maximum Correntropy Criterion.

The performance of ellipse fitting may significantly degrade in the presence of outliers, which can be caused by occlusion of the object, mirror reflection or other objects in the process of edge detection. In this paper, we propose an ellipse fitting method that is robust against the outliers, and thus maintaining stable performance when outliers can be present. We formulate an optimization problem for ellipse fitting based on the maximum entropy criterion (MCC), having the Laplacian as the kernel function from the well-known fact that the l -norm error measure is robust to outliers.

[Concomitant use of immobilized uridine-cytidine kinase and polyphosphate kinase for 5'-cytidine monophosphate production].

Uridine-cytidine kinase, an important catalyst in the compensation pathway of nucleotide metabolism, can catalyze the phosphorylation reaction of cytidine to 5'-cytidine monophosphate (CMP), but the reaction needs NTP as the phosphate donor. To increase the production efficiency of CMP, uridine-cytidine kinase gene from Thermus thermophilus HB8 and polyphosphate kinase gene from Rhodobacter sphaeroides were cloned and expressed in Escherichia coli BL21(DE3). Uridine-cytidine kinase was used for the generation of CMP from cytidine and ATP, and polyphosphate kinase was used for the regeneration of ATP.