Recent advances in biomolecule immobilization based on self-assembly: organic-inorganic hybrid nanoflowers and metal-organic frameworks as novel substrates.

In the past few years, the immobilization of biomolecules on hybrid nanoflowers and metal-organic frameworks (MOFs) via self-assembly synthesis has received much attention due to its simplicity, high efficiency, and a bright prospect of enhancing the stability, activity and even selectivity of biomolecules compared to conventional immobilization methods. In the synthesis of organic-inorganic hybrid nanoflowers, biomolecules used as organic components are simply mixed with metal ions which act as inorganic components to form flower-like nanocomposites, while in the self-assembly process of encapsulating biomolecules in MOFs (biomolecule@MOF composites), the biomolecules just need to be added to the precursor mixtures of MOFs, in which the biomolecules are therefore embedded in MOF crystals with small pores. In this review, we focus on the recent advances of these composites, especially in the synthesis strategies, mechanism and applications in biosensors, biomedicine, pollutant disposal, and industrial biocatalysis, and future perspectives are discussed as well.

Preparation of core-shell structured magnetic covalent organic framework nanocomposites for magnetic solid-phase extraction of bisphenols from human serum sample.

Core-shell structured magnetic covalent organic framework (FeO@COF) nanocomposites were synthesized via a facile approach at room temperature and explored as an absorbent for magnetic solid-phase extraction (MSPE) of bisphenols (BPs) from human serum sample. The as-prepared FeO@COF nanocomposites with core-shell structure possessed high specific surface area (181.36m/g), uniform mesoporous size (~ 3.

Facile synthesis of Ti -immobilized affinity silica nanoparticles for the highly selective enrichment of intact phosphoproteins.

Currently, great challenges to top-down phosphoproteomics lie in the selective enrichment of intact phosphoproteins from complex biological samples. Herein, we developed a facile approach for synthesis of Ti -immobilized affinity silica nanoparticles and applied them to the selective separation and enrichment of intact phosphoproteins based upon the principle of metal(IV) phosphate/phosphonate chemistry. The as-prepared affinity materials exhibited high selectivity and adsorption capacities for model phosphoproteins (328.

Protein-Metal Organic Framework Hybrid Composites with Intrinsic Peroxidase-like Activity as a Colorimetric Biosensing Platform.

Artificial enzyme mimetics have received considerable attention because natural enzymes have some significant drawbacks, including enzyme autolysis, low catalytic activity, poor recovery, and low stability to environmental changes. Herein, we demonstrated a facile approach for one-pot synthesis of hemeprotein-metal organic framework hybrid composites (H-MOFs) by using bovine hemoglobin (BHb) and zeolitic imidazolate framework-8 (ZIF-8) as a model reaction system. Surprisingly, the new hybrid composites exhibit 423% increase in peroxidase-like catalytic activity compared to free BHb.