Preparation of Antioxidant Peptides from Chicken Bone Proteins and the Influence of Their Compositional Characteristics on Antioxidant Activity.

Antioxidants play an important role in maintaining health and enhancing food stability by neutralizing free radicals. This study aimed to extract antioxidant peptides from white-feathered chicken bones through enzymatic hydrolysis, optimize the enzymatic hydrolysis conditions, and further investigate the relevance between the amino acid composition, molecular weight, and antioxidant activity of the resulting chicken bone hydrolysate. Alcalase was the most effective enzyme for hydrolyzing cooked chicken bones compared with papain, pepsin, and trypsin, yielding hydrolysates with the highest DH and ABTS radical scavenging activity.

Uricase from , a Candidate for Industrial Application of Reducing Uric Acid Content of Bean Products.

Microbial uricase is an essential enzyme in purine degradation and the development of low-purine food. High enzyme activity and an appropriate optimum pH must be established for low-purine food. Uricases from , , , , and were heterologously expressed in .

Principal component analysis-assisted zirconium-based metal-organic frameworks/DNA biosensor for the analysis of various phosphates.

Considering the diversity of phosphates and their pivotal roles in physiological processes, detection of various phosphates related to their metabolism is urgent but challenging. Herein, we design a biosensor with zirconium-based MOFs (Zr-MOFs) and fluorophore-modified single-stranded DNA (F-ssDNA) for the analysis of phosphates. Relying on the interaction between Zr clusters and phosphate backbone, F-ssDNA is anchored on the surface of Zr-MOFs, inducing fluorescence resonance energy transfer (FRET) and subsequently quenching the fluorescence of F-ssDNA.

Partitioning the Effects of Soil Legacy and Pathogen Exposure Determining Soil Suppressiveness via Induced Systemic Resistance.

Beneficial host-associated bacteria can assist plant protection against pathogens. In particular, specific microbes are able to induce plant systemic resistance. However, it remains largely elusive which specific microbial taxa and functions trigger plant immune responses associated with disease suppression.

Bio-organic soil amendment promotes the suppression of Ralstonia solanacearum by inducing changes in the functionality and composition of rhizosphere bacterial communities.

Stimulating the development of soil suppressiveness against certain pathogens represents a sustainable solution toward reducing pesticide use in agriculture. However, understanding the dynamics of suppressiveness and the mechanisms leading to pathogen control remain largely elusive. Here, we investigated the mechanisms used by the rhizosphere microbiome induces bacterial wilt disease suppression in a long-term field experiment where continuous application of bio-organic fertilizers (BFs) triggered disease suppressiveness when compared to chemical fertilizer application.

Placenta-specific 8 (PLAC8) mediates inflammation and mobility of the hPDLCs via MEK/ERK signaling pathway.

Background: Placenta-specific 8 (PLAC8) is reported to regulate cellular functions in the progression of various diseases. However, its role in periodontitis is still unclear.

Methods: Human periodontal ligament cells (hPDLCs) were treated with lipopolysaccharide of Porphyromonas Gingivalis (LPS-PG) to mimic periodontitis in vitro.

Soil microbiome manipulation triggers direct and possible indirect suppression against Ralstonia solanacearum and Fusarium oxysporum.

Soil microbiome manipulation can potentially reduce the use of pesticides by improving the ability of soils to resist or recover from pathogen infestation, thus generating natural suppressiveness. We simulated disturbance through soil fumigation and investigated how the subsequent application of bio-organic and organic amendments reshapes the taxonomic and functional potential of the soil microbiome to suppress the pathogens Ralstonia solanacearum and Fusarium oxysporum in tomato monocultures. The use of organic amendment alone generated smaller shifts in bacterial and fungal community composition and no suppressiveness.

Effects of foliar application of amino acid liquid fertilizers, with or without Bacillus amyloliquefaciens SQR9, on cowpea yield and leaf microbiota.

Leaf surface fertilization with liquid fertilizer produced from amino acids constitutes a potentially important source of nitrogen and is important for plant production. However, few reports have focused on the plant growth promotion by novel liquid fertilizers created by new amino acid resources, let alone the influence on leaf microbiota. In this study, the effects of liquid fertilizer, created by amino acids hydrolyzed from animal hairs with or without the PGPR strain Bacillus amyloliquefaciens SQR9, on crop yield and leaf microbiota were investigated.

Highly Sensitive and Precise Analysis of SF Decomposition Component CO by Multi-comb Optical-feedback Cavity Enhanced Absorption Spectroscopy with a 2.3 μm Diode Laser.

A ppb-level CO sensor based on multi-comb optical-feedback cavity enhanced absorption spectroscopy with a 2.3 μm diode laser was developed for SF decomposition analysis in electric power system. The effective optical path reached to 4.

Application of Self-Assembled Raman Spectrum-Enhanced Substrate in Detection of Dissolved Furfural in Insulating Oil.

Accurate detection of dissolved aging features in transformer oil is the key to judging the aging degree of oil-paper insulation. In this work, in order to realize in situ detection of furfural dissolved in transformer oil, silver nanoparticles were self-assembled on the surface of gold film with P-aminophenylthiophenol (PATP) as a coupling agent. Rhodamine-6G (R6G) was used as the probe molecule to test the enhancement effect.

High Performance Acetylene Sensor with Heterostructure Based on WO₃ Nanolamellae/Reduced Graphene Oxide (rGO) Nanosheets Operating at Low Temperature.

The development of functionalized metal oxide/reduced graphene oxide (rGO) hybrid nanocomposites concerning power equipment failure diagnosis is one of the most recent topics. In this work, WO₃ nanolamellae/reduced graphene oxide (rGO) nanocomposites with different contents of GO (0.5 wt %, 1 wt %, 2 wt %, 4 wt %) were synthesized via controlled hydrothermal method.

Application of Bacillus velezensis NJAU-Z9 Enhanced Plant Growth Associated with Efficient Rhizospheric Colonization Monitored by qPCR with Primers Designed from the Whole Genome Sequence.

Plant growth-promoting rhizobacteria (PGPR) have been reported to influence plant growth, yield, and nutrient uptake by an array of mechanisms. Uncovering the behavioral dynamics of PGPR is one of the most important issues necessary for understanding their functional performances. In this study, strain NJAU-Z9 which was found to possess complex functions and efficient rhizospheric colonization ability was selected from plenty of bacterial strains isolated randomly from the pepper rhizosphere soil and identified as Bacillus velezensis.

Self-assembly of fluorinated gradient copolymer in three-dimensional co-flow focusing microfluidic.

Hypothesis: The microfluidic technology can drive molecules to organize into aggregates with nano-structures, and gives a possibility to control aggregate morphologies by adjusting hydrodynamic parameters of microfluidics. COMSOL Multiphysics is a useful software to simulate the mixing situation of solutions in microfluidic. Here, experiments and simulation are combined to study the self-assembly of gradient copolymers in the microfluidic device.

Rhodium(II)-catalyzed intramolecular annulation of 1-sulfonyl-1,2,3-triazoles with pyrrole and indole rings: facile synthesis of N-bridgehead azepine skeletons.

A convenient and efficient synthetic method has been developed to construct highly functionalized N-bridgehead azepine skeletons, which are of great importance in biological and pharmaceutical industry. The reaction proceeds through a rhodium(II) azavinyl carbene intermediate, which initiated the intramolecular C-H functionalization with pyrrolyl and indolyl rings. A variety of azepine derivatives were obtained in moderate to good yields under mild reaction conditions with high chemoselectivity.