Prophylactic efficacy of an intranasal spray with 2 synergetic antibodies neutralizing Omicron.

BACKGROUNDAs Omicron is prompted to replicate in the upper airway, neutralizing antibodies (NAbs) delivered through inhalation might inhibit early-stage infection in the respiratory tract. Thus, elucidating the prophylactic efficacy of NAbs via nasal spray addresses an important clinical need.METHODSThe applicable potential of a nasal spray cocktail containing 2 NAbs was characterized by testing its neutralizing potency, synergetic neutralizing mechanism, emergency protective and therapeutic efficacy in a hamster model, and pharmacokinetics/pharmacodynamic (PK/PD) in human nasal cavity.

Effects of ozone combined with articular injection of sodium hyaluronate on patients with knee osteoarthritis and their inflammatory factors and hemorheological indices.

Objective: Knee osteoarthritis (KOA) is a common chronic progressive disease. We aimed to evaluate the effects of ozone combined with articular injection of sodium hyaluronate on KOA patients and their inflammatory factors and hemorheological indices.

Methods: A total of 292 KOA patients treated from October 2020 to October 2021 were randomly divided into observation and control groups (n=146).

QiShenYiQi pill inhibits atherosclerosis by promoting TTC39B-LXR mediated reverse cholesterol transport in liver.

Background: Tetranucleotide repeat domain protein 39B (TTC39B) was found to combine with ubiquitin ligase E3, and promote the ubiquitination modification of liver X receptor (LXR), which led to the inhibition of reverse cholesterol transport and development of atherosclerosis. QiShenYiQi pill (QSYQ) is a modern Chinese patent drug for treating ischemic cardiovascular diseases, the underlying mechanism is found to promote the expression of LXR-α/ ATP-binding cassette transporter G5 (ABCG5) in the liver of atherosclerotic mice.

Purpose: The aim of this study is to investigate the effect of QSYQ on TTC39B-LXR mediated reverse cholesterol transport in atherosclerotic mice.

miR-124 and VAMP3 Act Antagonistically in Human Neuroblastoma.

Neuroblastoma (NB) is the most common extracranial solid tumor that affects developing nerve cells in the fetus, infants, and children. miR-124 is a microRNA (miRNA) enriched in neuronal tissues, and VAMP3 (vesicle-associated membrane protein 3) has been reported to be an miR-124 target, although the relationship between NB and miR-124 or VAMP3 is unknown. Our current work identified that miR-124 levels are high in NB cases and that elevated miR-124 correlates with worse NB outcomes.

Transcriptome analysis reveals immune regulation in the spleen of koi carp (Cyprinus carpio Koi) during Aeromonas hydrophila infection.

A. hydrophila (Aeromonas hydrophila) is one of the most hazardous pathogenic microorganisms threatening the aquaculture industry and exhibits zoonotic-like characteristics. This study was designed to investigate the differential gene expression and pathway enrichment in the spleen of koi carp (Cyprinus carpio koi) upon A.

QiShenYiQi pill inhibits atherosclerosis by promoting reverse cholesterol transport PPARγ-LXRα/β-ABCA1 pathway.

Ethnopharmacological Relevance: QiShenYiQi pill (QSYQ), a Chinese compound medicine, originate from BuYangHuanWu decoction in the Qing dynasty, and has been used to treat ischemic cardiovascular diseases for more than two hundred years in China. Multi-central randomized double-blind controlled studies have proved that QSYQ has similar efficacy as enteric coated aspirin in the secondary prevention of myocardial infarction.

Aim Of Study: The aim of study was to explore the effect of QSYQ on reverse cholesterol transport (RCT) pathway during atherosclerosis.

A potent neutralizing antibody provides protection against SARS-CoV-2 Omicron and Delta variants via nasal delivery.

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is still rapidly spreading worldwide. Many drugs and vaccines have been approved for clinical use show efficacy in the treatment and prevention of SARS-CoV-2 infections. However, the emergence of SARS-CoV-2 variants of concern (VOCs), such as Delta (B.

Rh(III)-catalysed C-H/C-H cross-coupling of -aryl sulfoximines with thiophenes: facile access to [1]benzothieno[3,2-][1]benzothiophene (BTBT) and benzothiazines.

Reported herein is rhodium-catalysed oxidative C-H/C-H cross-coupling of -aryl sulfoximines with thiophenes a chelation-assisted strategy, which provides an efficient approach for the construction of [1]benzothieno[3,2-][1]benzothiophene (BTBT) and benzothiazine skeletons from easily available substrates. This protocol exhibits a good compatibility with halogen substituents, thus paving the way for further transformation to prepare various organic functional molecules. The resulting benzothiazine derivative shows a deep blue emission with Commission Internationale de 'Eclairage (CIE) coordinates of (0.

Ir(III)-catalyzed quadruple C-H activation of -arylimidazolium and diaryliodonium salts: facile access to polysubstituted imidazo[1,2-]phenanthridiniums.

Herein, N-heterocyclic carbene-directed Ir(III)-catalyzed cascade C-H arylation/annulation of -arylimidazolium with diaryliodonium salts has been accomplished for the first time a quadruple C-H activation strategy to construct imidazo[1,2-]phenanthridinium structures. This protocol overcomes the compatibility of three kinds of different C-H activations with high catalytic efficiency, which allows -unhindered -arylimidazoliums to undergo a diarylation/annulation reaction, affording a variety of polysubstituted imidazo[1,2-]phenanthridiniums. Neutral imidazo[1,2-]phenanthridines are also prepared a demethylation reaction of imidazo[1,2-]phenanthridiniums.

Phase-Controlled Synthesis of Pd-Se Nanocrystals for Phase-Dependent Oxygen Reduction Catalysis.

Searching for highly efficient oxygen reduction reaction (ORR) electrocatalysts for fuel cell technology, in which the crystal structure plays a powerful role in regulating the electrocatalysis, is urgent yet challenging. Herein, we have explored the active and stable Pd-Se alloy electrocatalysts with controlled phase toward alkaline ORR. The phase-controlled Pd-Se nanoparticles (NPs) show interesting phase-dependent electrocatalytic performance, in which the PdSe NPs/C exhibits much better ORR performance than its counterpart, PdSe NPs/C, and the commercial Pd/C and Pt/C.

A Generalized Surface Chalcogenation Strategy for Boosting the Electrochemical N Fixation of Metal Nanocrystals.

Electrocatalytic nitrogen reduction reaction (NRR) is a promising process relative to energy-intensive Haber-Bosch process. While conventional electrocatalysts underperform with sluggish paths, achieving dissociation of N brings the key challenge for enhancing NRR. This study proposes an effective surface chalcogenation strategy to improve the NRR performance of pristine metal nanocrystals (NCs).

Verbascoside inhibits progression of glioblastoma cells by promoting Let-7g-5p and down-regulating HMGA2 via Wnt/beta-catenin signalling blockade.

Glioblastoma (GBM) continues to show a poor prognosis despite advances in diagnostic and therapeutic approaches. The discovery of reliable prognostic indicators may significantly improve treatment outcome of GBM. In this study, we aimed to explore the function of verbascoside (VB) in GBM and its effects on GBM cell biological processes via let-7g-5p and HMGA2.

Pd(II)-Catalyzed Regioselective Multiple C-H Arylations of 1-Naphthamides with Cyclic Diaryliodonium Salts: One-Step Access to [4]- and [5]Carbohelicenes.

Highly efficient and rapid construction of carbohelicenes is a desired but challenging task, especially starting from simple substrates. Herein, Pd(II)-catalyzed regioselective multiple C-H arylations of 1-naphthamides with cyclic diaryliodoniums have been accomplished for the first time, which enables facile one-step syntheses of [4]- and [5]carbohelicenes from readily available starting materials. The resulting single and double [4]carbohelicenes emit violet to deep-blue fluorescence; [4,5,4]-fused triple carbohelicenes exhibit sky-blue emissions, and phenoxazine-modified [4]carbohelicene shows a delayed fluorescence emission.

Differential Inhibition of Target Gene Expression by Human microRNAs.

microRNAs (miRNAs) exert their functions by repressing the expression of their target genes, but most miRNA target genes are unknown, and the degree to which a miRNA differentially inhibits the expression of its targets is underappreciated. We selected human miR-1, miR-122, and miR-124 as representatives to investigate the reliability of miRNA target predictions and examine how miRNAs suppress their targets. We constructed miRNA target gene reporter libraries based on prediction programs TargetScan, miRanda, and PicTar, and performed large-scale reporter assays to directly evaluate whether and how strongly a predicted target gene is repressed by its miRNA.

Short- and long-term effects of manganese, zinc and copper ions on nitrogen removal in nitritation-anammox process.

This study provided a deep insight into the impacts of trace elements (Mn, Zn and Cu) on nitritation-anammox process. For short-term exposure, all the three elements could improve the nitrogen removal rate (NRR) and the optimal concentrations were 2.0 mg/L, 2.

[Treatment of lesions in mesencephalic aqueduct and around it by neuroendoscope].

Objective: To explore the surgical efficacy of lesions in mesencephalic aqueduct and around it with neuroendoscope.

Methods: A total of 14 cases with lesion underwent neuroendoscope. Endoscopic third ventriculostomy (ETV), excision or biopsy and mesencephalic aqueduct plasty were performed.

[Surgical treatment of neuroendoscopy with supported channel for hypertensive intraventricular hemorrhage].

Objective: To evaluate the value and efficacy of surgical treatment with neuroendoscopy with supported channel for hypertensive intraventricular hemorrhage (HIVH).

Methods: The clinical data of 32 patients with hypertensive intraventricular hemorrhage were retrospectively analyzed. And they underwent neuroendoscopy with supported channel.

Whole-genome sequencing in a patient with Charcot-Marie-Tooth neuropathy.

Background: Whole-genome sequencing may revolutionize medical diagnostics through rapid identification of alleles that cause disease. However, even in cases with simple patterns of inheritance and unambiguous diagnoses, the relationship between disease phenotypes and their corresponding genetic changes can be complicated. Comprehensive diagnostic assays must therefore identify all possible DNA changes in each haplotype and determine which are responsible for the underlying disorder.

[Treatment of lumbar intervertebral disc herniation accompanying with lumbar instability with internal fixation and spinal fusion].

Objective: To explore operative effect of lumbar intervertebral disc herniation accompanying with lumbar instability.

Methods: Form June 2000 to June 2006, 46 patients of lumbar intervertebral disc herniation accompanying with lumbar instability were treated with decompression through posterior approach, diskectomy, spinal fusion and vertebral pedicle internal fixation. Including 33 males and 13 females,the age was from 37 to 68 years with an average of 48 years.

[Aerobic nitrifying granulation fed with source-separated urine in sequencing batch reactor].

Morphological characteristics of aerobic nitrifying granules that were utilized to treat the diluted source-separated urine were investigated in two lab-scale sequencing batch reactors. In the first sequencing batch reactor, which was inoculated with nitrifying bacteria, the COD of effluent was markedly decreased while the nitrification rate was very low. Aerobic nitrifying granules were not appeared in the first bioreactor.

Influence of different substrates on the formation and characteristics of aerobic granules in sequencing batch reactors.

The effects of different substrates on the aerobic granulation process were studied using laboratory-scale sequencing batch reactors (SBRs). Four parallel granules sequencing batch reactors (GSBR): R1, R2, R3, and R4 were fed with acetate, glucose, peptone and fecula, respectively. Stable aerobic granules were successfully cultivated in R1, R2, R4, and smaller granules less than 500 microm were formed in R3.

Clustering genes using gene expression and text literature data.

Clustering of gene expression data is a standard technique used to identify closely related genes. In this paper, we develop a new clustering algorithm, MSC (Multi-Source Clustering), to perform exploratory analysis using two or more diverse sources of data. In particular, we investigate the problem of improving the clustering by integrating information obtained from gene expression data with knowledge extracted from biomedical text literature.

An eco-informatics tool for microbial community studies: supervised classification of Amplicon Length Heterogeneity (ALH) profiles of 16S rRNA.

Support vector machines (SVM) and K-nearest neighbors (KNN) are two computational machine learning tools that perform supervised classification. This paper presents a novel application of such supervised analytical tools for microbial community profiling and to distinguish patterning among ecosystems. Amplicon length heterogeneity (ALH) profiles from several hypervariable regions of 16S rRNA gene of eubacterial communities from Idaho agricultural soil samples and from Chesapeake Bay marsh sediments were separately analyzed.

Identification of 20 snoRNA-like RNAs from the primitive eukaryote, Giardia lamblia.

From a specialized cDNA library of Giardia lamblia, 20 snoRNA-like RNAs, including 16 box C/D sRNAs and four box H/ACA sRNAs, were first identified. The sRNAs were predicted to guide a total of 11 2'-O-methylation and four pseudouridylation sites on the G. lamblia rRNAs, respectively.