Integrated cytological and transcriptomic analysis reveals insights into pollen fertility in newly synthetic allohexaploids.

Trigenomic allohexaploids (AABBCC, 2n = 6x = 54) have great potential in oilseed breeding and genetic diversity. However, allohexaploids do not exist naturally, and the underlying mechanism regulating pollen fertility in artificially synthesized allohexaploids is still unclear. In this study, synthetic allohexaploids were produced by crossing allotetraploid (BBCC, 2n = 4x = 34) and diploid (AA, 2n = 2x = 20), followed by chromosome doubling.

Transcriptome Profiling Identifies Candidate Genes Contributing to Male and Female Gamete Development in Synthetic Allohexaploids.

Polyploidy plays a crucial role in plant evolution and speciation. The development of male and female gametes is essential to the reproductive capacity of polyploids, but their gene expression pattern has not been fully explored in newly established polyploids. The present study aimed to reveal a detailed atlas of gene expression for gamete development in newly synthetic allohexaploids that are not naturally existing species.