Increased cardiac myosin super-relaxation as an energy saving mechanism in hibernating grizzly bears.

Aim: The aim of the present study was to define whether cardiac myosin contributes to energy conservation in the heart of hibernating mammals.

Methods: Thin cardiac strips were isolated from the left ventricles of active and hibernating grizzly bears; and subjected to loaded Mant-ATP chase assays, X-ray diffraction and proteomics.

Main Findings: Hibernating grizzly bears displayed an unusually high proportion of ATP-conserving super-relaxed cardiac myosin molecules that are likely due to altered levels of phosphorylation and rod region stability.

Efficient construction of high-quality sulfonated porous aromatic frameworks by optimizing the swelling state of porous structures.

Conventional post-modification methods usually face the fundamental challenge of balancing the high content of functional groups and large surface area for porous organic polymers (POPs). The reason, presumably, stems from ineffective and insufficient swelling of the porous structure of POP materials, which is detrimental to mass transfer and modification of functional groups, especially with large-sized ones. It is important to note that significant differences exist in the porous structures of POP materials in a solvent-free state after thermal activation and solvent swelling state.

The Mediating Effect of Perceived Control Between Perceived Health Competence and Kinesiophobia in Elderly Patients after Coronary Artery Bypass Grafting.

Objective: To investigate the status quo of control attitudes, perceived health competence, and kinesiophobia in elderly patients with coronary disease after coronary artery bypass grafting (CABG), and to explore the mediating role of control attitudes in perceived health competence and fear of exercise.

Methods: By a convenient sampling method, 206 elderly patients with coronary heart disease who underwent CABG for the first time in the Department of Cardiovascular Surgery, First Affiliated Hospital of Anhui Medical University from June 1, 2021, to April 4, 2023, were investigated by using a general information questionnaire, the Perceived Health Competence Scale and Revised Control Attitudes Scale, and the Tampa Scale for Kinesiophobia to investigate the level of perceived health competence, perceived control, and kinesiophobia.

Results: Perceived health competence, control attitudes, and kinesiophobia scores were 25.

InterLabelGO+: unraveling label correlations in protein function prediction.

Motivation: Accurate protein function prediction is crucial for understanding biological processes and advancing biomedical research. However, the rapid growth of protein sequences far outpaces the experimental characterization of their functions, necessitating the development of automated computational methods.

Results: We present InterLabelGO+, a hybrid approach that integrates a deep learning-based method with an alignment-based method for improved protein function prediction.

An unexpected role of IL10 in mesoderm induction and differentiation from pluripotent stem cells: Implications in zebrafish angiogenic sprouting, vascular organoid development, and therapeutic angiogenesis.

Mesoderm induction is a crucial step for vascular cell specification, vascular development and vasculogenesis. However, the cellular and molecular mechanisms underlying mesoderm induction remain elusive. In the present study, a chemically-defined differentiation protocol was used to induce mesoderm formation and generate functional vascular cells including smooth muscle cells (SMCs) and endothelial cells (ECs) from human induced pluripotent stem cells (hiPSCs).

Evaluating the performance of carbon dioxide and methane observations from carbon-monitoring satellite products over China.

As the primary greenhouse gases contributing to climate change, carbon dioxide (CO₂) and methane (CH) play a critical role in the Earth's radiative balance and temperature regulation. Continuous and accurate satellite monitoring of CO₂ and CH₄ is essential for developing effective policies and strategies to mitigate their impacts. This study utilizes ground-based Fourier-transform infrared measurements from the Total Carbon Column Observing Network (TCCON) to evaluate the performance of current mainstream carbon-monitoring satellites over China, the world's largest carbon emitter.

LncRNA-SNHG16 Protects Against Oxidative Stress-Induced Vascular Endothelial Cell Injury in Cardiovascular Diseases by Regulating the miR-23a-3p-GLS-Glutamine Metabolism Axis.

Cardiovascular diseases are disorders of the heart and vascular system that cause high mortality rates worldwide. Vascular endothelial cell (VEC) injury caused by oxidative stress (OS) is an important event in the development of various cardiovascular diseases, including ischemic heart disease. This study aimed to investigate the critical roles and molecular mechanisms of long non-coding RNA (lncRNA) SNHG16 in regulating vascular endothelial cell injury under oxidative stress.

Investigating vertical distributions and photochemical indications of formaldehyde, glyoxal, and NO from MAX-DOAS observations in four typical cities of China.

Formaldehyde (HCHO), glyoxal (CHOCHO), and nitrogen dioxide (NO) are crucial in atmospheric photochemical processes at both surface and elevated altitudes. This study presents synchronous multi-axis differential optical absorption spectroscopy (MAX-DOAS) measurements of the vertical distributions of summertime HCHO, CHOCHO and NO in four representative megacities within the Beijing-Tianjin-Hebei (BTH), Yangtze River Delta (YRD), Sichuan Basin (SB), and Pearl River Delta (PRD) regions of China. The vertical distributions of HCHO and CHOCHO tended to occur at higher altitudes compared to NO, influenced by both primary emissions near the ground and photochemical oxidation processes at elevated altitudes.

Long-term spatiotemporal variations of ammonia in the Yangtze River Delta region of China and its driving factors.

This study focuses on the spatiotemporal distribution, urban-rural variations, and driving factors of ammonia Vertical Column Densities (VCDs) in China's Yangtze River Delta region (YRD) from 2008 to 2020. Utilizing data from the Infrared Atmospheric Sounding Interferometer (IASI), Generalized Additive Models (GAM), and the GEOS-Chem chemical transport model, we observed a significant increase of NH VCDs in the YRD between 2014 and 2020. The spatial distribution analysis revealed higher NH concentrations in the northern part of the YRD region, primarily due to lower precipitation, alkaline soil, and intensive agricultural activities.

FURNA: A database for functional annotations of RNA structures.

Despite the increasing number of 3D RNA structures in the Protein Data Bank, the majority of experimental RNA structures lack thorough functional annotations. As the significance of the functional roles played by noncoding RNAs becomes increasingly apparent, comprehensive annotation of RNA function is becoming a pressing concern. In response to this need, we have developed FURNA (Functions of RNAs), the first database for experimental RNA structures that aims to provide a comprehensive repository of high-quality functional annotations.

A large-scale assessment of sequence database search tools for homology-based protein function prediction.

Sequence database searches followed by homology-based function transfer form one of the oldest and most popular approaches for predicting protein functions, such as Gene Ontology (GO) terms. These searches are also a critical component in most state-of-the-art machine learning and deep learning-based protein function predictors. Although sequence search tools are the basis of homology-based protein function prediction, previous studies have scarcely explored how to select the optimal sequence search tools and configure their parameters to achieve the best function prediction.

The role and research progress of macrophages after heart transplantation.

Since the 60s of the 20th century, heart transplantation has been the best treatment for patients with end-stage heart failure. Due to the increasing number of patients, how to expand the number of donor organs and enhance immune compatibility has become an urgent problem to be solved at this stage. Although current immunosuppression is effective, its side effects are also quite obvious, such as opportunistic infections and malignant tumors.

A literature review on signaling pathways of cervical cancer cell death-apoptosis induced by Traditional Chinese Medicine.

Ethnopharmacological Relevance: Cervical cancer (CC) is a potentially lethal disorder that can have serious consequences for a woman's health. Because early symptoms are typically only present in the middle to late stages of the disease, clinical diagnosis and treatment can be challenging. Traditional Chinese medicine (TCM) has been shown to have unique benefits in terms of alleviating cancer clinical symptoms, lowering the risk of recurrence after surgery, and reducing toxic side effects and medication resistance after radiation therapy.

The cGAS-STING pathway in cardiovascular diseases: from basic research to clinical perspectives.

The cyclic guanosine monophosphate (GMP)-adenosine monophosphate (AMP) synthase-stimulator of interferon genes (cGAS-STING) signaling pathway, an important component of the innate immune system, is involved in the development of several diseases. Ectopic DNA-induced inflammatory responses are involved in several pathological processes. Repeated damage to tissues and metabolic organelles releases a large number of damage-associated molecular patterns (mitochondrial DNA, nuclear DNA, and exogenous DNA).

Optimized production of full-length PCV2d virus-like particles in Escherichia coli: A cost-effective and high-yield approach for potential vaccine antigen development.

Porcine circovirus type 2 (PCV2) is a globally prevalent infectious pathogen affecting swine, with its capsid protein (Cap) being the sole structural protein critical for vaccine development. Prior research has demonstrated that PCV2 Cap proteins produced in Escherichia coli (E. coli) can form virus-like particles (VLPs) in vitro, and nuclear localization signal peptides (NLS) play a pivotal role in stabilizing PCV2 VLPs.

Small nucleolar RNA host gene 18 controls vascular smooth muscle cell contractile phenotype and neointimal hyperplasia.

Aims: Long non-coding RNA (LncRNA) small nucleolar RNA host gene 18 (SNHG18) has been widely implicated in cancers. However, little is known about its functional involvement in vascular diseases. Herein, we attempted to explore a role for SNHG18 in modulating vascular smooth muscle cell (VSMC) contractile phenotype and injury-induced neointima formation.

Comparative analysis of robotically-assisted versus conventional sternotomy approach in left atrial-myxoma resection: A single-center retrospective observational study.

Background: Minimally invasive surgery has emerged as a favorable alternative to conventional surgery for various cardiac conditions. This study aimed to compare the perioperative outcomes and follow-up results of the robotic approach versus the sternotomy approach for left atrial myxoma (LAM) resection.

Method: We retrospectively analyzed the perioperative outcomes and follow-up results of 94 patients who underwent left atrial myxoma resection using either the sternotomy approach (n = 64) or the robotic approach (n = 30) at our center between January 2017 and April 2023.

Risk factors for prolonged postoperative ICU stay in the patients with Stanford type A aortic dissection.

Objective: To investigate the independent risk factors for postoperative prolonged ICU stay in patients with Stanford type A aortic dissection (TAAD) and assess the clinical outcomes of prolonged ICU stay.

Method: The clinical data of 100 patients with TAAD admitted to the Department of Cardiovascular Surgery, First Affiliated Hospital of Anhui Medical University from December 2018 to September 2022 were retrospectively collected and analyzed. Patients were divided into two groups, based on the postoperative ICU stay (7 days as the threshold), regular ICU stay group (< 7 days) and prolonged ICU stay group (≥ 7 days).

Live streaming E-commerce platform characteristics: Influencing consumer value co-creation and co-destruction behavior.

Live streaming with goods is developing rapidly as an emerging platform economic model. Consumers can interact with the e-commerce platform in real-time and get a good shopping experience. This in turn generates value co-creation behaviors such as word-of-mouth communication.

TM-search: An Efficient and Effective Tool for Protein Structure Database Search.

The quickly increasing size of the Protein Data Bank is challenging biologists to develop a more scalable protein structure alignment tool for fast structure database search. Although many protein structure search algorithms and programs have been designed and implemented for this purpose, most require a large amount of computational time. We propose a novel protein structure search approach, TM-search, which is based on the pairwise structure alignment program TM-align and a new iterative clustering algorithm.

Proteomic screens of SEL1L-HRD1 ER-associated degradation substrates reveal its role in glycosylphosphatidylinositol-anchored protein biogenesis.

Endoplasmic reticulum-associated degradation (ERAD) plays indispensable roles in many physiological processes; however, the nature of endogenous substrates remains largely elusive. Here we report a proteomics strategy based on the intrinsic property of the SEL1L-HRD1 ERAD complex to identify endogenous ERAD substrates both in vitro and in vivo. Following stringent filtering using a machine learning algorithm, over 100 high-confidence potential substrates are identified in human HEK293T and mouse brown adipose tissue, among which ~88% are cell type-specific.