The role of Wnt/β-catenin pathway for skin-derived precursors differentiating into corneal endothelial cell-like cells.

Bullous keratopathy is a serious blinding eye disease requiring corneal endothelial transplantation. However, the lack of cornea donors forced us to search for new sources of functional corneal endothelial cells (CECs). In our previous study, we have successfully differentiated the SKPs into CEC-like cells with unclear mechanism.

Long-Term Observation and Sequencing Analysis of SKPs-Derived Corneal Endothelial Cell-Like Cells for Treating Corneal Endothelial Dysfunction.

Corneal endothelial dysfunction is a principal cause of visual deficiency. Corneal transplantation is the most effective treatment for corneal endothelial dysfunction. However, a severe shortage of available donor corneas or human corneal endothelial cells (HCECs) remains a global challenge.

Clinical observations and considerations in the treatment of Terson syndrome using 23G vitrectomy.

Purpose: This retrospective study analyzed the results of 23G vitrectomy for the treatment of intraocular hemorrhage in patients with Terson syndrome. The pathogenesis of Terson syndrome and the timing of vitrectomy are discussed.

Methods: Eight eyes of eight patients were included in the study.

Diplopia in a patient with Hashimoto's thyroiditis: A case report and literature review.

Background: A case report of Hashimoto's thyroiditis-associated ophthalmopathy that masqueraded as double elevator palsy in 1 eye.

Case Presentation: A 54-year-old woman presented to our strabismus clinic with diplopia for 1 year. She was diagnosed with double elevator palsy in the left eye.

Three-Dimensional Construction of a Rabbit Anterior Corneal Replacement for Lamellar Keratoplasty.

The aim of this study was to construct a rabbit anterior corneal replacement for transplantation using acellular porcine corneal matrix (APCM) and rabbit epithelial or stromal cells. APCM was prepared from fresh porcine cornea treated with 0.5% (wt.

[Construction of corneal endothelium deficiency model by cryo-injury and its application].

Objective: To set up an animal model of corneal endothelium deficiency and test the bio-safety and pump function of B4G12 cells with this model.

Methods: Thirty SD rats were divided into cryo-injury group and cryo-injury with B4G12 cell transplantation group. Models of corneal endothelium deficiency were created by cryo-injury with liquid nitrogen, and then B4G12 cells were transplanted into the eyes by anterior chamber injection.

Derivation of corneal endothelial cell-like cells from rat neural crest cells in vitro.

The aim of this study was to investigate the feasibility of inducing rat neural crest cells (NCC) to differentiate to functional corneal endothelial cell (CEC)-like cells in vitro. Rat NCC were induced with adult CEC-derived conditioned medium. Immunofluorescence, flow cytometry and real time RT-PCR assay were used to detect expression of the corneal endothelium differentiation marker N-cadherin and transcription factors FoxC1 and Pitx2.

A human corneal endothelium equivalent constructed with acellular porcine corneal matrix.

Background & Objectives: Artificial corneal endothelium equivalents can not only be used as in vitro model for biomedical research including toxicological screening of drugs and investigation of pathological corneal endothelium conditions, but also as potential sources of grafts for corneal keratoplasty. This study was aimed to demonstrate the feasibility of constructing human corneal endothelium equivalents using human corneal endothelial cells and acellular porcine corneal matrix.

Methods: Porcine corneas were decellularized with sodium dodecyl sulphate (SDS) solution.