Impact of Graphene Exposure on Microbial Activity and Community Ecosystem in Saliva.

Graphene-based nanomaterials (GMs) are served as great promising agents for the prevention and therapy of infectious diseases. However, their dental applications remain to be evaluated, especially under the context of the oral microbial community. Here, we examined the exposure-response of salivary bacterial community to two types of GMs, that is, graphene oxide (GO) and GO-silver nanoparticles (AgNPs).

Graphene oxide-silver nanocomposites modulate biofilm formation and extracellular polymeric substance (EPS) production.

Biofilms with positive and negative actions ubiquitously affect medical infections, environmental remediation and industrial processes. However, it remains challenging to control the growth of harmful biofilms as well as to exploit the use of beneficial biofilms. Here we investigated the effect of an antibacterial graphene oxide-silver nanoparticles (GO-AgNPs) composite on Pseudomonas aeruginosa biofilm formation.

Framework Nucleic Acid-Mediated Pull-Down MicroRNA Detection with Hybridization Chain Reaction Amplification.

Gastric cancer remains a disease of high mortality worldwide due to its poor prognosis. Previous studies have shown that microRNAs (miRNAs) are effective biomarkers for early diagnosis of gastric cancer. To realize sensitive detection of related miRNAs for improved early diagnosis, classification, and survival prognosis of gastric cancer, herein we developed a framework nucleic acid (FNA)-mediated microarray for quantitative analysis of multiple miRNAs.

Programming biosensing sensitivity by controlling the dimension of nanostructured electrode.

Development of new nanostructured materials has shown high impact for improving the performance of chemical and biological sensors. In this work, we show that by controlling the dimensions of the gold flower microelectrode (GFME), it is possible to regulate detection sensitivity of a sensor for rapid analysis of chemical species. A ~13-fold increase in sensitivity was achieved by enlarging the dimension of GFMEs from 70 to 330 μm, whereas the response dynamics are dimension-independent, with the signal attaining saturation ~20 s.

Real-Time Continuous Identification of Greenhouse Plant Pathogens Based on Recyclable Microfluidic Bioassay System.

The development of a real-time continuous analytical platform for the pathogen detection is of great scientific importance for achieving better disease control and prevention. In this work, we report a rapid and recyclable microfluidic bioassay system constructed from oligonucleotide arrays for selective and sensitive continuous identification of DNA targets of fungal pathogens. We employ the thermal denaturation method to effectively regenerate the oligonucleotide arrays for multiple sample detection, which could considerably reduce the screening effort and costs.