Integrated multi-omics analysis reveals insights into Chinese forest musk deer () genome evolution and musk synthesis.

Among the artiodactyls, male animals belonging to the Family Moschidae have a unique tissue, the musk gland, with the capability of musk synthesis. However, the genetic basis of musk gland formation and musk production are still poorly understood. Here, musk gland tissues from two juvenile and three adult Chinese forest musk deer () were utilized to analyze genomic evolution events, evaluate mRNA profiles and investigate cell compositions.

Complete mitochondrial genome of the golden takin ().

The complete mitochondrial genome sequence of was 16 662-bp long, containing 13 protein-coding genes (PCGs), 22 tRNA genes, 2 rRNA genes and a control region. All of the PCGs begin with the typical ATN start codon. Stop codons TAA and AGA are present in the PCGs; exceptions are ND2, COIII, ND3 and ND4, which possess incomplete termination codon (T--).

Expression and Purification of glutathione transferase-small ubiquitin-related modifier-metallothionein fusion protein and its neuronal and hepatic protection against D-galactose-induced oxidative damage in mouse model.

The present study aimed to produce and pathophysiologically evaluate the metallothionein (MT) fusion protein. A recombinant plasmid containing DNA segment coding the pET-glutathione transferase (GST)-small ubiquitin-related modifier (SUMO)-MT fusion protein was inserted into Escherichia coli for expression. The expression level of the fusion protein was very high, reaching to 38.

High-level expression and purification of Tat-haFGF19-154.

Human acidic fibroblast growth factor (haFGF) stimulates repair and regeneration of central and peripheral nerves after various injuries. However, it is unable to cross the blood-brain barrier (BBB). To produce a therapeutic haFGF with cell-permeable activity, we fused the haFGF(19-154) gene with Tat-PTD.