A novel and facile oxygen-activated time-temperature indicator with wide temperature monitoring range and good stability based on the laccase-like nanozyme.

Background: Time-Temperature Indicator (TTI) is an indicator device for real-time monitoring of the thermal history of the product. Due to the enzymatic reactions are affected by both time and temperature, enzymatic TTIs have been extensively studied and developed in recent years. However, enzymatic TTIs contain biologically active molecules (enzymes), which require high storage and use conditions.

A novel hybrid sensor array based on the polyphenol oxidase and its nanozymes combined with the machine learning based dual output model to identify tea polyphenols and Chinese teas.

A novel sensor array was developed based on the enzyme/nanozyme hybridization for the identification of tea polyphenols (TPs) and Chinese teas. The enzyme/nanozyme with polyphenol oxidase activity can catalyze the reaction between TPs and 4-aminoantipyrine (4-AAP) to produce differences in color, and the sensor array was thus constructed to accurately identify TPs mixed in different species, concentrations, or ratios. In addition, a machine learning based dual output model was further used to effectively predict the classes and concentrations of unknown samples.

A colorimetric sensor array based on peroxidase activity nanozyme for the highly efficient differential sensing of tea polyphenols and Tieguanyin adulteration.

Tieguanyin (TGY) is one of top ten famous teas in China, but in the process of brand building there is the phenomenon of falsehood, thus harming the interests of consumers. To solve theadulterate problem of TGY, a colorimetric sensor array (CSA) based onperoxidase activity of nanozyme was constructed. Nanozymes can catalyze 3,3',5,5'-tetramethylbenzidine (TMB) to 3,3',5,5'-tetramethyl -[1,1'-bis(cyclohexyl)]-2,2',5,5'-tetraene-4,4'-diimine (oxTMB), while the tea polyphenols (TPs) can inhibit this process, and the degree of inhibition varies significantly with the reaction time.

Application of Oblique Lateral Interbody Fusion Combined with Bridge-Locking Cage in Adjacent Segment Disease After Lumbar Fusion.

Objective: Adjacent segment disease (ASD) is considered any abnormal process that develops in the mobile segment next to spinal fusion, accompanied by related symptoms. To evaluate the efficacy and complications of oblique lateral interbody fusion (OLIF) combined with bridge-locking fusion on ASD after lumbar fusion.

Methods: A total of 35 ASD patients who required re-operation after lumbar fusion in our hospital from March 2014 to March 2020 were retrospectively analyzed, among which 13 cases (seven males and six females; 62.

Resveratrol promotes axonal regeneration after spinal cord injury through activating Wnt/β-catenin signaling pathway.

Background: Spinal cord injury (SCI) is characterized by autonomic dysreflexia, chronic pain, sensory and motor deficits. Resveratrol has shown potential neuroprotective function in several neurodegenerative diseases' models. However, if resveratrol could improve the function recovery after SCI and the further mechanism have not been investigated.

Therapeutic efficacy of TACE I seed implantation and its combination with intra-tumor injection of cisplatin for the treatment of hepatocellular carcinoma.

Background: This study aimed to compare the therapeutic efficacy of transcatheter arterial chemoembolization (TACE) combined with either I seed implantation or I seed implantation and intra-tumor injection of cisplatin in treating hepatocellular carcinoma (HCC).

Methods: A total of 100 patients with HCC were analyzed. The control group (n = 50) received TACE combined with I seed implantation therapy.

Assessment of the feasibility of TACE combined with intratumoral injection of cisplatin in hepatocellular carcinoma.

The feasibility of transcatheter arterial chemo-embolization (TACE) combined with intratumoral injection of cisplatin as treatment for hepatocellular carcinoma. 30 cases receiving TACE were denoted the TACE group, another 30 cases receiving TACE combined with an intratumoral multi-point injection of cisplatin were denoted the TACE/cisplatin group. Cases with partial remission/complete remission (PR/CR) were analyzed using 2 tests; alpha fetoprotein (AFP), aspartate amino transferase (AST), total bilirubin (TBIL), erythrocyte, and platelet levels were detected and the differences between two groups were analyzed using the Student's t-test; cases with complications, including intrahepatic metastasis (IM), upper gastrointestinal bleeding (UGB), and liver failure were also counted.

hBMP-2 and hTGF-β1 expressed in implanted BMSCs synergistically promote the repairing of segmental bone defects.

Objective: To evaluate the effects of co-expressing hBMP-2 and hTGF-β1 in BMSCs (bone marrow-derived mesenchymal stem cells) on the repairing process of radial segmental defects in rats.

Methods: BMSCs were infected with a high titer recombinant adenovirus carrying hTGF-βl and/or hBMP-2 genes. Expression of exogenous genes in BMSCs was confirmed by RT-PCR and ELISA assays.

Improvement of antibiotic productivity by knock-out of dauW in Streptomyces coeruleobidus.

Daunorubicin (DNR) is an important anthracycline antibiotic. Its biosynthesis pathway has been well understood, however, the regulation of DNR biosynthesis needs further investigations. An ORF cloned between drrB and dnrX from the genome of a DNR producer, Streptomyces coeruleobidus DM, was named dauW and designated as an orthologous gene with dnrW and drrD.

[Research on carboxymethyl chitosan acting as the adjuvant for implantable degradable microspheres].

As a kind of biomaterial, carboxymethyl chitosan (CMC) has excellent biodegradable and bioacceptable capabilities using. This study was aimed to probe into the feasibility of CMC to prepare the implantable sustained release Ciprofloxacin Hydrochloride (CPX) microspheres(MS), and to go further into the pharmaceutic technology, the morphology and the characteristics of in vitro release of the microspheres. First, we prepared the microspheres by emulsification and cross-linking technology.

Use of a self-designed bladder controller for restoring bladder function in paraplegic dogs.

Objective: To evaluate the results of a self-designed bladder controller for restoring micturition function in paraplegic dogs.

Methods: The spinal cords of 4 dogs were transected above the cone. Electrodes were implanted in S2 bilaterally and connected to the subcutaneous receivers for external activation.

[Bladder controller in bladder function reconstruction of paraplegic dogs].

Objective: To observe whether bladder controller can restore bladder function in paraplegic dogs.

Methods: Four dogs were studied after their T(10) spinal cord was transected. Electrodes implanted around S(2) bilaterally were connected to subcutaneous recievers.

Surgical management for sciatic nerve injury.

Objective: To summarize surgical treatments and their corresponding curative effects on sciatic nerve injuries.

Methods: Surgical treatments on sciatic nerve injury were performed in 28 patients from January 1990 to July 2000. The treatments included neurolysis, neurolysis plus partial nerve anastomosis, nerve anastomosis and nerve transplantation.

[Clinical study on chitosan in prevention of knee adhesion after patellar operation].

Objective: To investigate the clinical effect of chitosan in prevention of knee dysfunction due to adhesion after operation for patellar fracture.

Methods: From March to October 1999, 40 cases of patellar fracture were treated by internal fixation, with intra-articular injection of 2% chitosan in only 24 cases after fixation and with no chitosan injection in 16 cases (control group). The function of the knee joint, including extension and flexion, was evaluated 1 month and 1 year after operation respectively.