Antifungal activity of bamemacrolactine C against Talaromyces marneffei and its possible mechanisms of action.

Aims: The present study aims to investigate the in vitro antifungal activity and mechanism of action of bamemacrolactine C (BAC), a new 24-membered macrolide compound, against Talaromyces marneffei.

Methods And Results: The test drug BAC initially demonstrated antifungal activity through a paper disk diffusion assay, followed by determination of the minimum inhibitory concentration value of 35.29 μg ml-1 using microdilution.

Antiproliferative effects of resorcylic acid lactones from the Beibu Gulf coral-derived fungus GXIMD 02512 on prostate cancer cells.

Four novel resorcylic acid lactones (RALs), curvulomycins A-D (1-4), and six known congeners were isolated from the Beibu Gulf coral-derived fungus GXIMD 02512. Their structures including absolute configurations were established by extensive spectroscopic analyses along with experimental and calculated ECD spectra. Structurally, compound 3 harbors a unique γ-pyrone moiety rarely found in the natural RAL family.

Cadophorin C, a new cyclic heptapeptide isolated from a mangrove-derived fungus sp. GXIMD 03101.

One new cyclic heptapeptide, cadophorin C (), and one known analogue cadophorin B () were isolated from the mangrove-derived fungus sp. GXIMD 03101 from the mangrove  L. The chemical structure of was elucidated by comprehensive analysis of the spectroscopic data, including 1D and 2D NMR and HRESIMS, and the known compound was identified by comparing the data with literature values.

Talaketides A-G, linear polyketides with prostate cancer cytotoxic activity from the mangrove sediment-derived fungus Talaromyces sp. SCSIO 41027.

Seven novel linear polyketides, talaketides A-G (1-7), were isolated from the rice media cultures of the mangrove sediment-derived fungus Talaromyces sp. SCSIO 41027. Among these, talaketides A-E (1-5) represented unprecedented unsaturated linear polyketides with an epoxy ring structure.

Recent Advances in Anti-Inflammatory Compounds from Marine Microorganisms.

Marine microbial secondary metabolites with diversified structures have been found as promising sources of anti-inflammatory lead compounds. This review summarizes the sources, chemical structures, and pharmacological properties of anti-inflammatory natural products reported from marine microorganisms in the past three years (2021-2023). Approximately 252 anti-inflammatory compounds, including 129 new ones, were predominantly obtained from marine fungi and they are structurally divided into polyketides (51.

High-efficiency breeding of Bacillus siamensis with hyper macrolactins production using physical mutagenesis and a high-throughput culture system.

Macrolactins have attracted considerable attention due to their value and application in medicine and agriculture. However, poor yields severely hinder their broader application in these fields. This study aimed to improve macrolactins production in Bacillus siamensis using a combined atmospheric and room-temperature plasma mutagenesis and a microbial microdroplet culture system.

Millmerranones G, a meroterpene isolated from a mangrove-derived fungus sp. GXIMD 03004.

One new meroterpene derivative, millmerranones G (), and three known analogues (-) were identified from the mangrove-derived fungus sp. GXIMD 03004, which was isolated from the leaves of mangrove L. collected from Beibu Gulf in China.

Mauritone A, a new polyketide from a fungal-bacterial symbiont GXIMD 04541/ GXIMD 04532.

A new polyketide, mauritone A () with six known polyketides curvulone B (), curvularin (), 12-oxocurvularin (), (10,15)-10,11-dehydrocurvularin (), (11,15)-11-hydroxycurvularin (), and (11,15)-11-hydroxycurvularin () were isolated from the fungal-bacterial symbiont GXIMD 04541/ GXIMD 04532 derived from . Their structures were elucidated by extensive spectral analysis. All compounds (-) were evaluated for their anti-inflammatory effects.

New Cyclic Pentapeptides from the Mangrove-Derived GXIMD 03099.

Four new cyclic pentapeptides, avellanins D-G (-), together with four known compounds (-), were isolated from a mangrove-derived GXIMD 03099 fungus from L. Their structures were elucidated by analysis of HRESIMS, NMR, and ESI-MS/MS data. Their absolute configurations were determined by X-ray diffraction analysis and Marfey's method.

Secofumitremorgins C and D, a pair of atropisomers from saltern-derived fungus GXIMD00544.

A pair of atropisomers secofumitremorgins C () and D (), together with fifteen known alkaloids (), were isolated from a saltern-derived fungus GXIMD00544. The structures of atropisomers and were elucidated by the detailed spectroscopic data, chemical reaction and quantum chemical calculations. Compounds and displayed antifungal spore germination effects against plant pathogenic fungus associated with sugarcane sp.

Lactobacillus fermentum WC2020 increased the longevity of Caenorhabditis elegans via JNK-mediated antioxidant pathway.

Lactobacillus fermentum can exert antiaging effects, but their roles are strain-specific, and little is known about the molecular mechanisms in some strains. This study investigated the antiaging effects of L. fermentum WC2020 (WC2020) isolated from Chinese fermented pickles and the underlying mechanism of the action in Caenorhabditis elegans.

Peniditerpenoids A and B: Oxidized Indole Diterpenoids with Osteoclast Differentiation Inhibitory Activity from a Mangrove-Sediment-Derived sp.

An unprecedented di--indole diterpenoid, peniditerpenoid A (), and a rare -oxide-containing indole diterpenoid derivative, peniditerpenoid B (), together with three known ones (-), were obtained from the mangrove-sediment-derived fungus sp. SCSIO 41411. Their structures were determined by the analysis of spectroscopic data, quantum chemical calculations, and X-ray diffraction analyses.

Brominated Depsidones with Antibacterial Effects from a Deep-Sea-Derived Fungus sp.

Eleven new brominated depsidones, namely spiromastixones U-Z5 (-) along with five known analogues (-), were isolated from a deep-sea-derived fungus sp. through the addition of sodium bromide during fermentation. Their structures were elucidated by extensive analysis of the spectroscopic data including high-resolution MS and 1D and 2D NMR data.

Carneusones A-F, Benzophenone Derivatives from Sponge-Derived Fungus GXIMD00543.

Six benzophenone derivatives, carneusones A-F (-), along with seven known compounds (-) were isolated from a strain of sponge-derived marine fungus GXIMD00543. Their chemical structures were elucidated by detailed spectroscopic data and quantum chemical calculations. Compounds , , and exhibited moderate anti-inflammatory activity on NO secretion using lipopolysaccharide (LPS)-induced RAW 264.

New Alkaloids and Steroids from Hydranth of Goniopora columna Corals and Their Inhibiting Lung Cancer Cell Activities.

A new alkaloids, aplysingoniopora A (1), and new configuration pregnane type steroid compound, 9,17-α-pregn-1,4,20-en-3-one (2), and two known pregnane type steroid compounds (3 and 4) were isolated from hydranth of Goniopora columna corals. The compounds structures and absolute configurations were determined by extensive spectroscopic analysis, MS data, single-crystal X-ray diffraction analysis and quantum chemical calculation. The anticancer effect of the compounds were explored in human non-small-cell lung cancer (NSCLC) A549 cell lines.

Discovery of Anti-Hypercholesterolemia Agents Targeting LXRα from Marine Microorganism-Derived Natural Products.

A strategy integrating molecular docking with LXRα and phenotypic assays was adopted to discover anti-hypercholesterolemia agents in a small library containing 205 marine microorganism-derived natural products, collected by our group in recent years. Two fumitremorgin derivatives, 12,13-dihydroxyfumitremorgin C () and tryprostatin A (), were identified as potential LXRα agonists, by real-time qPCR and Western blot (WB) analysis, together with a surface plasmon resonance (SPR) assay. The anti-hypercholesterolemic effects of and , together with their mechanisms, were investigated in depth using different cell and mouse models, among which the study of LXRα is of crucial importance.

Recent Advances in Chemistry and Bioactivities of Secondary Metabolites from the Genus .

fungi is one of the greatest and most complex genera in Hyphomycetes, comprising 130 species of marine and terrestrial sources. The past decades have witnessed substantial chemical and biological investigations on the diverse secondary metabolites from the species. To date, over 600 compounds with abundant chemical types as well as a wide range of bioactivities have been obtained from this genus, attracting considerable attention from chemists and pharmacologists.

New Polyene Macrolide Compounds from Mangrove-Derived Strain GXIMD 06359: Isolation, Antifungal Activity, and Mechanism against .

Mangrove-derived actinomycetes represent a rich source of novel bioactive natural products in drug discovery. In this study, four new polyene macrolide antibiotics antifungalmycin B-E (-), along with seven known analogs (-), were isolated from the fermentation broth of the mangrove strain GXIMD 06359. All compounds from this strain were purified using semi-preparative HPLC and Sephadex LH-20 gel filtration while following an antifungal activity-guided fractionation.

KLF5 inhibits the migration and invasion in cervical cancer cell lines by regulating SNAI1.

Background: Epithelial-mesenchymal transition (EMT) is an important biological process by which malignant tumor cells to acquire migration and invasion abilities. This study explored the role of KLF5 in the EMT process of in cervical cancer cell lines.

Objective: Krüpple-like factor 5 (KLF5) is a basic transcriptional factor that plays a key role in cell-cycle arrest and inhibition of apoptosis.

The quantitative pyrrole protection of l-phenylalanine/l-phenylalaninol in aqueous media and rationally updating the mechanisms of the Clauson-Kaas reaction through DFT study.

The classical Paal-Knorr reaction is a prominent tool that can be adopted under biocompatible conditions covering various γ-dicarbonyls for either chemical biology or drug discovery. Meanwhile, the relatively mild conditions for larger molecules within biological systems have not been employed to obtain -substituted pyrrole derivatives from simpler chiral amino acids/alcohols. The Clauson-Kaas methodology of a standard two-phase acidic mixture buffered with acetate salts was generally required for the time-consuming catalytic condensation of 2,5-dimethoxytetrahydrofuran and fast removal of pyrrolyl products after formation to inhibit their racemization.

Sonneralignan A, a new lignan from the fruit of mangrove .

A new lignan, sonneralignan A (), along with two known lignan compounds, (+)-lariciresinol-9---D-glucopyranoside () and (-)isolariciresinol-9---D-glucopyranoside () were isolated from the n-butanol extract of the mangrove fruit. The structures of the compounds were elucidated on the basis of extensive spectral analysis. The evaluation of activity showed that compound exhibited significant anti-aging activity, which extended the mean lifespan of by up to 19.

Two New Species of Talaromyces Sect. Trachyspermi Discovered in China.

Two new species of sect. Trachyspermi isolated from soil are proposed, namely, T. albidus (ex-type AS3.

Anti-Vibrio potential of natural products from marine microorganisms.

The emergence of drug-resistant Vibrio poses a serious threat to aquaculture and human health, thus there is an urgent need for the discovery of new related antibiotics. Given that marine microorganisms (MMs) are evidenced as important sources of antibacterial natural products (NPs), great attention has been gained to the exploration of potential anti-Vibrio agents from MMs. This review summarizes the occurrence, structural diversity, and biological activities of 214 anti-Vibrio NPs isolated from MMs (from 1999 to July 2022), including 108 new compounds.

Structural Elucidation of a Glucan from by Its Degraded Products and Preparation of Its Sulfated Derivative as an Anticoagulant.

Echinoderms have been attracting increasing attention for their polysaccharides, with unique chemical structure and enormous potential for preparing drugs to treat diseases. In this study, a glucan (TPG) was obtained from the brittle star . Its structure was elucidated by physicochemical analysis and by analyzing its low-molecular-weight products as degraded by mild acid hydrolysis.