Complete genome sequence of a penicillin-resistant subsp. from a tonsillitis patient.

We report the complete genome sequence of a penicillin-resistant subsp. isolate, AJ79, from a tonsillitis patient. The AJ79 genome consists of a chromosome (2,440,359 bp) and plasmid (9,887 bp), providing insights into the genetic basis of penicillin resistance in and its implications for treating tonsillitis.

Development of SacB-based counterselection for efficient allelic exchange in .

, prevalent in the oral cavity, is significantly linked to overall human health. Our molecular comprehension of its role in oral biofilm formation and its interactions with the host under various pathological circumstances has seen considerable advancements in recent years, primarily due to the development of various genetic tools for DNA manipulation in this bacterium. Of these, counterselection-based unmarked in-frame mutation methods have proved notably effective.

Discovery, characterization, and structure of a cofactor-independent histidine racemase from the oral pathogen Fusobacterium nucleatum.

Fusobacterium nucleatum is an oral commensal bacterium that can act as an opportunistic pathogen and is implicated in diseases such as periodontitis, adverse pregnancy outcomes, colorectal cancer, and Alzheimer's disease. F. nucleatum synthesizes lanthionine for its peptidoglycan, rather than meso-2,6-diaminopimelic acid (DAP) used by most Gram-negative bacteria.

Development of SacB-based Counterselection for Efficient Allelic Exchange in .

Unlabelled: , prevalent in the oral cavity, is significantly linked to overall human health. Our molecular comprehension of its role in oral biofilm formation and its interactions with the host under various pathological circumstances has seen considerable advancements in recent years, primarily due to the development of various genetic tools for DNA manipulation in this bacterium. Of these, counterselection-based unmarked in-frame mutation methods have proved notably effective.

Fusobacterium nucleatum subsp. animalis comes to the spotlight in oral diseases.

Krieger et al.'s study in this issue of Cell Host & Microbe reveals that Fusobacterium nucleatum subsp. animalis strains, previously underestimated, are significant in disease-affected oral areas.

Evaluation of Enoyl-ACP Reductase (FabK) as a Narrow-Spectrum Drug Target.

, a pathobiont inhabiting the oral cavity, contributes to opportunistic diseases, such as periodontal diseases and gastrointestinal cancers, which involve microbiota imbalance. Broad-spectrum antimicrobial agents, while effective against infections, can exacerbate dysbiosis. This necessitates the discovery of more targeted narrow-spectrum antimicrobial agents.

Grit and Second Language Learning Engagement: The Mediating Role of Affect Balance.

The study of the relationship between key psychological attributes of learners and their engagement in second language (L2) learning helps to understand the critical personality mechanisms influencing language learning. The present study examined the L2 learning engagement from the perspectives of grit (i.e.

Healthcare 5.0: A secure and distributed network for system informatics in medical surgery.

Introduction: Health records serve not only as a database of a patient's health history and treatment process but also as a crucial tool for doctors to diagnose and treat patients. However, the storage and sharing of these records are sensitive issues as they involve maintaining patient privacy and ensuring data transparency, security, and interoperability between different parties. Challenges to achieving these goals in the current surgical process can impact the allocation of medical resources and surgical outcomes.

Modeling the Health Impact and Cost-Effectiveness of a Combined Schoolgirl HPV Vaccination and Cervical Cancer Screening Program in Guangdong Province, China.

Low human papillomavirus (HPV) vaccine uptake is a key barrier to cervical cancer elimination. We aimed to evaluate the health impact and cost-effectiveness of introducing different HPV vaccines into immunization programs and scaling up the screening program in Guangdong. We used a dynamic compartmental model to estimate the impact of intervention strategies during 2023-2100.

DeepmdQCT: A multitask network with domain invariant features and comprehensive attention mechanism for quantitative computer tomography diagnosis of osteoporosis.

In the medical field, the application of machine learning technology in the automatic diagnosis and monitoring of osteoporosis often faces challenges related to domain adaptation in drug therapy research. The existing neural networks used for the diagnosis of osteoporosis may experience a decrease in model performance when applied to new data domains due to changes in radiation dose and equipment. To address this issue, in this study, we propose a new method for multi domain diagnostic and quantitative computed tomography (QCT) images, called DeepmdQCT.

Use of CRISPR interference for efficient and rapid gene inactivation in .

Gene inactivation by creating in-frame deletion mutations in is time consuming, and most fusobacterial strains are genetically intractable. Addressing these problems, we introduced a riboswitch-based inducible CRISPR interference (CRISPRi) system. This system employs the nuclease-inactive Cas9 protein (dCas9), specifically guided to the gene of interest by a constantly expressed single-guide RNA (sgRNA).

The respiratory enzyme complex Rnf is vital for metabolic adaptation and virulence in .

This paper illuminates the significant question of how the oral commensal adapts to the metabolically changing environments of several extra-oral sites such as placenta and colon to promote various diseases as an opportunistic pathogen. We demonstrate here that the highly conserved itrogen-ixation complex, commonly known as Rnf complex, is key to fusobacterial metabolic adaptation and virulence. Genetic disruption of this Rnf complex causes global defects in polymicrobial interaction, biofilm formation, cell growth and morphology, hydrogen sulfide production, and ATP synthesis.

Language Nativeness Modulates Physiological Responses to Moral vs. Immoral Concepts in Chinese-English Bilinguals: Evidence from Event-Related Potential and Psychophysiological Measures.

Morality has been an integral part of social cognition and our daily life, and different languages may exert distinct impacts on human moral judgment. However, it remains unclear how moral concept is encoded in the bilingual brain. This study, therefore, aimed to explore the emotional and cognitive involvement of bilingual morality judgement by using combined event-related potential (ERP) and psychophysiological (including skin, heart, and pulse) measures.

SR-POSE: A Novel Non-Contact Real-Time Rehabilitation Evaluation Method Using Lightweight Technology.

Rehabilitation movement assessment often requires patients to wear expensive and inconvenient sensors or optical markers. To address this issue, we propose a non-contact and real-time approach using a lightweight pose detection algorithm-Sports Rehabilitation-Pose (SR-Pose), and a depth camera for accurate assessment of rehabilitation movement. Our approach utilizes an E-Shufflenet network to extract underlying features of the target, a RLE-Decoder module to directly regress the coordinate values of 16 key points, and a Weight Fusion Unit (WFU) module to output optimal human posture detection results.

Use of CRISPR interference for efficient and rapid gene inactivation in .

Unlabelled: Gene inactivation via creating in-frame deletion mutations in is time-consuming, and most fusobacterial strains are genetically intractable. Addressing these problems, we introduced a riboswitch-based inducible CRISPRi system. This system employs the nuclease-inactive Cas9 protein (dCas9), specifically guided to the gene of interest by a constantly expressed single guide RNA (sgRNA).

Development of a xylose-inducible promoter and riboswitch combination system for manipulating gene expression in .

Inducible gene expression systems are important for studying bacterial gene function, yet most exhibit leakage. In this study, we engineered a leakage-free hybrid system for precise gene expression controls in by integrating the xylose-inducible expression system with the theophylline-responsive riboswitch. This innovative method enables concurrent control of target gene expression at both transcription and translation initiation levels.

Beyond Valence and Arousal: The Role of Age of Acquisition in Emotion Word Recognition.

Although the age of acquisition (AoA) effect has been established in numerous studies, how emotion word processing is modulated by AoA, along with affective factors, such as valence and arousal, is not well understood. Hence, the influence of age of acquisition (AoA), valence, and arousal on Chinese emotion word recognition was investigated through two experiments. Experiment 1 ( = 30) adopted a valence judgment task to explore the roles of valence and AoA in emotion word recognition, whereas Experiment 2 ( = 30) used a lexical decision task to examine AoA and arousal effects.

What is an Emotion-label Word? Emotional Prototypicality (EmoPro) Rating for 1,083 Chinese Emotion Words and Its Relationships with Psycholinguistic Variables.

The present study offered the emotion prototypicality (EmoPro) ratings for 1,083 Chinese emotion words. EmoPro measures the extent to which an emotion word refers to an emotion. Emotion words with high EmoPro are representative emotion-label words, so EmoPro provides an objective evaluation of defining an emotion-label word.

The respiratory enzyme complex Rnf is vital for metabolic adaptation and virulence in .

A prominent oral commensal and opportunistic pathogen, can traverse to extra-oral sites such as placenta and colon, promoting adverse pregnancy outcomes and colorectal cancer, respectively. How this anaerobe sustains many metabolically changing environments enabling its virulence potential remains unclear. Informed by our genome-wide transposon mutagenesis, we report here that the highly conserved Rnf complex, encoded by the gene cluster, is key to fusobacterial metabolic adaptation and virulence.

Development of a Xylose-Inducible Promoter and Riboswitch Combination System for Manipulating Gene Expression in .

Inducible gene expression systems are important for studying bacterial gene function, yet most exhibit leakage. In this study, we engineered a leakage-free hybrid system for precise gene expression controls in by integrating the xylose-inducible expression system with the theophylline-responsive riboswitch. This innovative method enables concurrent control of target gene expression at both transcription and translation initiation levels.

Spoken Word Recognition across Language Boundary: ERP Evidence of Prosodic Transfer Driven by Pitch.

Extensive research has explored the perception of English lexical stress by Chinese EFL learners and tried to unveil the underlying mechanism of the prosodic transfer from a native tonal language to a non-native stress language. However, the role of the pitch as the shared cue by lexical stress and lexical tone during the transfer remains controversial when the segmental cue (i.e.

On the superiority of a combination of aerobic and resistance exercise for fibromyalgia syndrome: A network meta-analysis.

Background: Fibromyalgia syndrome is the second most common chronic diffuse pain disorder and can have a lasting negative impact on the quality of life, pain, and physical function of people. Exercise therapy is an important component of the treatment of fibromyalgia, but there was not a consensus understanding of the effect of various exercise programs on the quality of life, pain, and physical function of people with fibromyalgia syndrome. This study aimed to compare three exercise programs (aerobic exercise, resistance exercise, and a combination of aerobic and resistance exercise) in their effectiveness in improving quality of life, relieving muscle pain, and enhancing physical function in patients with fibromyalgia.

Genetic Determinants of Hydrogen Sulfide Biosynthesis in Fusobacterium nucleatum Are Required for Bacterial Fitness, Antibiotic Sensitivity, and Virulence.

The Gram-negative anaerobe Fusobacterium nucleatum is a major producer of hydrogen sulfide (HS), a volatile sulfur compound that causes halitosis. Here, we dissected the genetic determinants of HS production and its role in bacterial fitness and virulence in this important member of the oral microbiome. F.