Incidence and clinical features of HHV-7 detection in lower respiratory tract in patients with severe pneumonia: a multicenter, retrospective study.

Purpose: The significance of detecting human herpesvirus 7 (HHV-7) in the lower respiratory tract of patients with severe pneumonia is unclear. This study aims to evaluate the clinical characteristics and prognosis of detecting HHV-7 in the lower respiratory tract of patients with severe pneumonia.

Methods: Patients with severe pneumonia requiring invasive mechanical ventilation and underwent commercial metagenomic next-generation sequencing (mNGS) testing of bronchoalveolar lavage fluid from January 2019 to March 2023 were enrolled in 12 medical centers.

Label-Free Analysis of Exosomes with Hairpin Structure-Mediated Multiple Signal Amplification Strategy.

Considering the crucial role of exosomes in various biological processes and disease diagnosis, development of label-free and sensitive exosome detection methods is in urgent demand. Herein, we develop an exponential amplification-based method pining the hope on sensitive exosomes detection in a label-free manner. In this method, a designed SMB (Streptavidin magnetic bead)-capture probe is utilized to specially recognize CD63 protein on the surface of exosome.

Metabolomic Characterization of a cf. Cyanobacterium from the South China Sea Reveals Wenchangamide A, a Lipopeptide with In Vitro Apoptotic Potential in Colon Cancer Cells.

Metabolomics can be used to study complex mixtures of natural products, or secondary metabolites, for many different purposes. One productive application of metabolomics that has emerged in recent years is the guiding direction for isolating molecules with structural novelty through analysis of untargeted LC-MS/MS data. The metabolomics-driven investigation and bioassay-guided fractionation of a biomass assemblage from the South China Sea dominated by a marine filamentous cyanobacteria, cf.

Sesquiterpenes from inhibiting the proliferation of synoviocytes.

Two new sesquiterpenes, litseachrandaevanes C and D ( and ), together with five known sesquiterpenes (), were isolated from the stems of (Hance) Merr. Their structures were elucidated using comprehensive spectroscopic methods. The inhibitory effect of all compounds on the proliferation of primary synovial cells was evaluated.