USP15 inhibits hypoxia-induced IL-6 signaling by deubiquitinating and stabilizing MeCP2.

Methyl-CpG binding protein 2 (MeCP2) is an important X-linked DNA methylation reader and a key heterochromatin organizer. The expression level of MeCP2 is crucial, as indicated by the observation that loss-of-function mutations of MECP2 cause Rett syndrome, whereas an extra copy spanning the MECP2 locus results in MECP2 duplication syndrome, both being progressive neurodevelopmental disorders. Our previous study demonstrated that MeCP2 protein expression is rapidly induced by renal ischemia-reperfusion injury (IRI) and protects the kidney from IRI through transcriptionally repressing the interleukin-6 (IL-6)/signal transducer and activator of transcription 3 signaling pathway.

Tyrosine phosphorylation of both STAT5A and STAT5B is necessary for maximal IL-2 signaling and T cell proliferation.

Cytokine-mediated STAT5 protein activation is vital for lymphocyte development and function. In vitro tyrosine phosphorylation of a C-terminal tyrosine is critical for activation of STAT5A and STAT5B; however, the importance of STAT5 tyrosine phosphorylation in vivo has not been assessed. Here we generate Stat5a and Stat5b tyrosine-to-phenylalanine mutant knockin mice and find they have greatly reduced CD8 T-cell numbers and profoundly diminished IL-2-induced proliferation of these cells, and this correlates with reduced induction of Myc, pRB, a range of cyclins and CDKs, and a partial G1→S phase-transition block.

Development and validation of a machine learning-based early prediction model for massive intraoperative bleeding in patients with primary hepatic malignancies.

Background: Surgical resection remains the primary treatment for hepatic malignancies, and intraoperative bleeding is associated with a significantly increased risk of death. Therefore, accurate prediction of intraoperative bleeding risk in patients with hepatic malignancies is essential to preventing bleeding in advance and providing safer and more effective treatment.

Aim: To develop a predictive model for intraoperative bleeding in primary hepatic malignancy patients for improving surgical planning and outcomes.

Student Loss: Towards the Probability Assumption in Inaccurate Supervision.

Noisy labels are often encountered in datasets, but learning with them is challenging. Although natural discrepancies between clean and mislabeled samples in a noisy category exist, most techniques in this field still gather them indiscriminately, which leads to their performances being partially robust. In this paper, we reveal both empirically and theoretically that the learning robustness can be improved by assuming deep features with the same labels follow a student distribution, resulting in a more intuitive method called student loss.

Efficacy and safety of electroacupuncture treatment in the prevention of negative moods in healthy young men after 30 h of total sleep deprivation: study protocol for a single-center, single-blind, parallel-arm, randomized clinical trial.

Background: Sleep deprivation (SD) among young adults is a major public health concern. In humans, it has adverse effects on mood and results in serious health problems. Faced with SD, persons may take precautionary measures to try and reduce their risk.

Sulfur-doped g-CN nanosheets for photocatalysis: Z-scheme water splitting and decreased biofouling.

In this study, an S-doped g-CN nanosheet was prepared as a photocatalyst for effective oxygen evolution reaction. Sulfur plays a crucial role in S-doped g-CN not only in increasing the charge density but also in reducing the energy band gap of S-doped g-CN via substitution of nitrogen sites. S-doped g-CN can serve as an oxygen-evolved photocatalyst, when combined with Ru/SrTiO:Rh in the presence of [Co(bpy)] as an electron mediator, enables photocatalytic overall water splitting under visible light irradiation with hydrogen and oxygen production rates of 24.

Colorimetric assay for cyanide and cyanogenic glycoside using polysorbate 40-stabilized gold nanoparticles.

This study describes a simple method for the selective and sensitive detection of cyanide and endogenous biological cyanide using polysorbate 40-stabilized gold nanoparticles.

[Atmospheric adjacency effect correction of ETM images].

It is an important precondition to retrieve the ground surface reflectance exactly for improving the subsequent product of remote sensing images and the quantitative application of remote sensing. However, because the electromagnetic wave is scattered by the atmosphere during its transmission from the ground surface to the sensor, the electromagnetic wave signal of the target received by the sensor contained the signal of the background. The adjacency effect emerges.

[Changes of the concentration of serum ischemia modified albumin and high sensitivity C-reactive protein in type 2 diabetic patients with retinopathy].

Objective: To explore the changes of the concentration of serum ischemia modified albumin (IMA) and high sensitivity C-reactive protein (hs-CRP) in type 2 diabetic patients with retinopathy (DR).

Methods: The concentration of serum IMA and hs-CRP in DR patients were determined by ELISA and rate nephelometry and compared with those in 83 no-DR (NDR) patients and 72 controls. The concentration of serum IMA and hs-CRP in 40 proliferative diabetic retinopathy (PDR) patients were compared with those in 39 no-PDR (NPDR) patients.

[Research on pixel decomposition of QuickBird multi-spectrum band images].

In the condition that the DN(Digital Number) value images of ground-atmosphere system recorded by the QuickBird artificial satellite sensor were taken as the experimental datum, the equation of pixel decomposition was structured, abiding by energy conservation law and the proportional relationship between the sum of 1-4 band integral radiance and the panchromatic band (Pan) integral radiance. By using the equation gained above, every pixel of 1-4 band images was decomposed into sixteen pixels. So the high resolution 1-4 band radiance images with the resolution of 0.

Integrated microfluidic system for electrochemical sensing of urinary proteins.

This study reports a new microfluidic system integrated with a microfluidic control module and a micro electrochemical module for detection of urinary proteins. The integrated microsystem can automatically detect proteins in urine with a high sensitivity. The microfluidic control module consists of a new two-way, spiral-shape micropump which can transport the urine samples to the sensing regions.

Purification and enrichment of virus samples utilizing magnetic beads on a microfluidic system.

This study reports a new microfluidic system with three integrated functional devices for pumping, mixing and separation of bio-samples by utilizing micro-electro-mechanical-systems technology. By using antibody-conjugated magnetic beads, the developed system can be used to purify and enrich virus samples such that the subsequent detection of viruses can be performed with a higher sensitivity. The target viruses were first captured by the antibody coated onto the magnetic beads by using a rotary micromixer which performed the incubation process.

GA binding protein regulates interleukin 7 receptor alpha-chain gene expression in T cells.

The interleukin 7 receptor alpha-chain (IL-7Ralpha) is essential for T cell development in both humans and mice and for B cell development in mice. Whereas the transcription factor PU.1 regulates IL-7Ralpha expression in mouse pro-B cells via a GGAA motif, we demonstrate here that GA binding protein (GABP) bound to this site and was essential in the regulation of IL-7Ralpha expression in T cells, where PU.