Effect of methotrexate exposure at middle gestation on the inner plate of the ocular cup and lens in the rat fetus.

Pregnant rats were treated intraperitoneally with a single dose of methotrexate (MTX) 90 mg/kg on gestation day (GD) 13, and fetal eyeballs were examined time-dependently from GD 13.5 to 15.5.

A rat model of vascular dementia for evaluating Chinese medicine prescriptions.

Objective: To develop a new model of vascular dementia for evaluating Chinese medicine prescriptions.

Methods: Eighty-eight male Wistar rats were randomly divided into 4 groups. At d, d, d, d (n=20, 20, 24, 24) during fatty-feeding, rats in each group were further divided into 10 or 12 subgroups (n=2), respectively.