Publications by authors named "Cheng-Wei Xia"
Prevention and management of postoperative deep vein thrombosis in lower extremities of patients with gastrointestinal tumor.
World J Gastrointest Surg
October 2024
Article Synopsis
- - Deep vein thrombosis (DVT) poses a serious risk for patients after gastrointestinal cancer surgery, especially for those with higher age and body mass index (BMI); effective preventive measures are essential to avoid complications like DVT and pulmonary embolism (PE).
- - A study involving 100 patients assessed a combined DVT prevention protocol—including risk assessments, mechanical support, pharmacological intervention, and early mobilization—with the goal of determining its impact on reducing DVT rates post-surgery.
- - Results showed a 7% DVT incidence and a 1% occurrence of PE among participants, with a high adherence rate of 92% to the protocol; age, BMI, and longer surgery duration were identified as significant risk factors
[A case report of Crohn's disease in a child with trisomy 8].
Zhongguo Dang Dai Er Ke Za Zhi
September 2024
The patient is a 12-year-old male who has experienced recurrent perianal abscesses for over 10 years, along with recurrent oral ulcers and deformities in the joints of hands and feet. Gastrointestinal endoscopy and capsule endoscopy revealed multiple ulcers in the digestive tract. Combined with his histopathological examinations, the patient was diagnosed with Crohn's disease.
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- Human caliciviruses, especially norovirus genotype GII.4, are significant causes of viral diarrhea in children, as highlighted by a study in China.
- Among 295 children with acute diarrhea, 10.85% tested positive for HuCV, with most being HBGA secretors or partial secretors, while only a small percentage of asymptomatic children tested positive.
- The findings suggest that blood type O may be linked to a higher risk of GII.3 infection, but due to the small sample size, additional research is needed for confirmation.
Article Synopsis
- A study conducted in Nanjing in 2013 aimed to identify the major viruses responsible for hand, foot and mouth disease (HFMD) in hospitalized children and examine their clinical and genetic features.
- The research found that 68.5% of throat swabs tested positive for enteroviruses, with coxsackievirus A6 (CVA6) being the most prevalent strain, followed by enterovirus 71 (EV71) and others.
- Patients infected with CVA6 exhibited atypical symptoms compared to those with EV71, and the study highlighted the presence of genetically distinct strains of CVA6 circulating in the region.
Article Synopsis
- Researchers used PCR to amplify and sequence the genome of human bocavirus 2 (HBoV2) from fecal samples collected in 2010, resulting in a 5444 bp genome (HBoV2-NC).
- Phylogenetic analysis indicated that HBoV2-NC is most closely related to the HBoV2 Lanzhou strain.
- The study also predicted that HBoV2-NC contains inverted terminal repeats with a typical stem-loop structure, which is characteristic of other parvoviruses, and some additional flanking sequences were identified through linker-PCR.
[Molecular and epidemiological study on among children under 5 years old in Nanjing].
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
February 2012
Objective: To study the infected information, clinical symptom and molecular epidemiological characteristics of HuCV infection among children under 5 years old in Nanjing.
Methods: In Nanjing Children's Hospital of Nanjing Medical University from July 2010 to June 2011, we collected 428 stool specimens from children with diarrhea and 428 asymptomatic controls. Human Calicivirus were tested by using RT-PCR.
Article Synopsis
- A high-throughput DNA sequencing method revealed a new porcine bocavirus (PBoV), identified as PBoV3C, in 19.6% of fecal samples from healthy piglets.
- PBoV3C has a nearly complete genome sequence of 5235 bp and exhibits genome characteristics similar to other known bocaviruses, showing a genomic similarity of 78% to 81% compared to other strains.
- The study highlights significant diversity and complexity in PBoVs and suggests a standardized naming system based on the VP1 gene for better classification.
Rapid and broad diagnostic methods are needed for the identification of viral agents of gastroenteritis. In this study, we used Luminex xMAP technology to develop a multiplexed assay for the simultaneous identification of major enteric viral pathogens, including rotavirus A (RVA), noroviruses (NoVs) (including genogroups GI and GII), sapoviruses (SaV), human astrovirus (HAstV), enteric adenoviruses (EAds), and human bocavirus 2 (HBoV2). The analytical sensitivity allowed detection of 10(3) (EAds, HBoV2, and RVA) and 10(4) (NoV GI and GII, SaV, and HAstV) copies per reaction mixture.
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- Acute gastroenteritis (AGE) is a major cause of death in children, with Human bocavirus 2 (HBoV2) recently being linked to its occurrence, although previous data has been limited.
- The study involved 632 children with diarrhea and 162 healthy controls in Lanzhou, China, analyzing various viruses, including HBoV2, using Real-time PCR for detection and quantification.
- Results showed HBoV2 was present in 20.4% of gastroenteritis cases but had a weaker association with AGE compared to other viruses, and infection with HBoV2 didn't worsen symptoms, indicating it likely doesn't cause AGE.
Article Synopsis
- The study investigates the role of noroviruses (especially GII-4) in causing acute gastroenteritis among adults in Beijing, revealing that 19.4% of specimens tested positive for NVs.
- Researchers identified new recombinant norovirus types and noted significant mutations in the capsid protein of the GII-4/Den Haag strain that may help it evade immune responses.
- The findings suggest that noroviruses are a major cause of gastroenteritis in Chinese adults and highlight the potential for recombination hotspots in noroviruses to contribute to their evolution and persistence.
[Detection and molecular characterization of human parechovirus (HPeV) in children with acute gastroenteritis].
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
February 2011
Article Synopsis
- The study aimed to investigate human parechovirus (HPeV) in stool samples from children under 5 with acute gastroenteritis.
- A real-time PCR test identified HPeV in 27 out of 306 children, with a higher incidence in autumn and a notable peak in July, especially in children older than 2.
- The findings align with previous reports on HPeV epidemiology, and the researchers emphasize the importance of ongoing large-scale surveillance for HPeV, as HPeV3 was notably absent in their samples.
[Molecular and epidemiological study on viral diarrhea among infants in Lanzhou].
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
February 2011
Objective: To study the epidemiologic characteristics of viral diarrhea in children under 5 years old in Lanzhou, understand the four major virus in children of distribution.
Methods: In the first hospital of Lanzhou university from Jul 2009 to Jun 2010,we collected 290 stool specimens from children with diarrhea and 114 asymptomatic controls. Rotavirus was detected by ELISA,further strain characterization was carried out by nested PCR.
To develop and optimize a simultaneous detection method of RotavirusA, Norovirus GI, GII, Sapovirus, human astrovirus, enteric adenoviruses and HBoV2 with GenomeLab GeXP analysis system. The sensitivity was verified to be 10(4) copies/microL with plasmids containing the viral targets in triplicate on different days, and no cross-reaction with enterovirus71, human Parechovirus and PicobirnavirusII was observed. Finally, we successfully developed a high throughout, rapid and maneuverable multiplex RT-PCR assay for simultaneous detection of seven viruses related with viral gastroenteritis, which provide a novel method for the molecular diagnosis of diarrhea-associated virus.
View Article and Find Full Text PDFHuman bocavirus 2 (HBoV2) is a parvovirus that has been recently identified in stool samples from children. Any association between the virus and clinical disease is unclear. A rapid, reliable diagnostic method is necessary to address this issue.
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- A new group of bocavirus has been discovered in piglet stool samples, showing a notable prevalence of 12.59%.
- Two nearly complete genomes of these viruses, named porcine bocavirus 1 and 2 (PBoV1 and PBoV2), have been sequenced, revealing high similarity to other members of the bocavirus genus.
- Phylogenetic analysis classified these new viruses alongside known bocaviruses and identified unique proteins, which may enhance the understanding of this viral genus.
[Sequence the complete sequence of bocavirus I with SISPA-PCR].
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
February 2010
Objective: To sequence the complete sequence of bocavirus I with sequence independent single primer amplification (SISPA-PCR).
Methods: To exclude the co-effection samples, all clinical samples of diarrhea cases were screened with special primers of rotavirus, astrovirus, adenovirus, calicivirus and bocavirus I. The virus were enriched through ultracentrifugation.
[Detection of Aichi virus in stool samples from children in Lanzhou].
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
February 2010
Article Synopsis
- The study aimed to identify and analyze the Aichi virus in both diarrhea cases and healthy children in Lanzhou, exploring its potential link to infant diarrhea.
- Utilizing RT-PCR methods, researchers found a very low detection rate of Aichi virus (0.06%) in hospitalized children with diarrhea, with no virus found in healthy children; the virus detected belonged to genotype B.
- The findings indicate the presence of B genotype Aichi virus in China, highlighting the need for further research on its characteristics and role in illness.
[Analysis on epidemiologic characteristics of viral diarrhea among infants in Taiyuan, Shanxi province, 2007-2008].
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
February 2010
Objective: To study the epidemiologic characteristics of virus-induced acute diarrhea in children under 5 years old in Taiyuan, Shanxi province.
Methods: Stool specimens and clinical data were collected from 346 inpatients with acute diarrhea from children less than 5 years old. Rotavirus-positive specimens were identified by ELASA kit.
From November 2008 to January 2009, a sharp increase of diarrhea in children in Guangdong province appeared, we randomly collected 53 stool specimens from out-patient children with dirrhea in 3 major hospitals (Guangzhou City Children's Hospital, Shenzhen Baoan District Maternal and Child Health Hospital, First Affiliated Hospital of Shantou University). Rotavirus and calicivirus were screened by ELISA and RT-PCR. We found 29 cases of rotavirus infection with diverse serotypes.
View Article and Find Full Text PDF[A method of detect the virus which caused diarrhea].
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
October 2009
Objective: Building a method which can examines virus pathogenic in gastroenteritis excrement specimen.
Methods: Choosing six positive specimens which tested in our laboratory, include adenovirus, calicivirus, rotavirus, bocavirus, astrovirus and enterovirus. Through sequence-independent single primer amplification(SISPA) constructs a gene bank.
Objective: Gastroenteritis is a major cause of childhood morbidity and mortality worldwide. Adenovirus AdV is recognized to be one of the most important pathogens associated with severe dehydrating gastroenteritis. Studies reported elsewhere have shown that about 8%-10% of cases with infantile diarrhea are caused by AdV and in some areas AdV diarrhea even occurred in the form of outbreaks.
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- A study collected stool samples from 1,195 children with acute diarrhea in Lanzhou, China, between 2001 and 2007 to analyze human calicivirus (HucV) infections.
- Of these samples, 117 tested positive for HucV, with a significant increase in infection rates noted in 2006-2007.
- The genetic analysis revealed that 86 specimens were norovirus and 10 were sapovirus, with GII/4 being the dominant strain, which evolved through several subtypes, particularly becoming the prevalent variant 2006b in Lanzhou.
[Detection of human parechovirus in children hospitalized for acute gastroenteritis].
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
April 2009
Article Synopsis
- * Using real-time PCR, researchers found HPeV in 24 out of 99 children, with various types present, predominantly HPeV1.
- * The findings suggest HPeV is common among these children in China, highlighting the need for further research to understand its impact on gastroenteritis.
Background: Gastroenteritis is a major cause of childhood morbidity and mortality worldwide. Rotavirus, human caliciviruses (HucV), adenovirus, and astrovirus are recognized as common etiologies of acute gastroenteritis.
Objectives: To use antigen detection and molecular methods to determine the viral etiology of childhood diarrhea in Lanzhou, China, 2005-2007.