[Effect of Codonopsis Radix maintained with sulfur fumigation on immune function in mice].

Objective: To investigate the immune function of mice being given the extract of Codonopsis Radix maintained with sulfur fumigation.

Methods: Mice were divided into five groups. Except the normal control group, the mice were fed with the extract of Codonopsis Radix maintained with sulfur fumigation at the high,medium and low doses, as well as medium dose of Codonopsis Radix maintained with low-temperature vacuum method, respectively.

[Effectiveness of different maintenance methods for codonopsis radix].

Objective: To observe different maintenance methods including vacuum-packing, storage together with tobacco, storage together with fennel, ethanol steam and sulfur fumigation for the protection of Codonopsis Radix against mildew and insect damage, and to analyze the content of polysaccharide and flavonoids of Codonopsis Radix tested in this studies, so as to look for the scientific maintenance methods replacing traditional sulfur fumigation.

Methods: Except for the sulfur fumigation, naturally air-dried Codonopsis Radix was used to investigate the maintenance effectiveness of the above methods, respectively. Mildew was observed by visual inspection, and the content of polysaccharide and flavonoids were determined by ultra-violet and visible spectrophotometer.

[Chemical constituents from Gentiana striata].

Objective: To study the chemical constituents of Gentiana striata.

Methods: The constituents were isolated from the whole herb of Gentiana striata by recrystalization, silica gel column chromatography, polyamide column chromatography and Sephadex LH-20,etc. Their structures were elucidated through MS, 1H-NMR, 13C-NMR.

NMR and X-ray crystallography, complementary tools in structural proteomics of small proteins.

NMR spectroscopy and X-ray crystallography, the two primary experimental methods for protein structure determination at high resolution, have different advantages and disadvantages in terms of sample preparation and data collection and analysis. It is therefore of interest to assess their complementarity when applied to small proteins. Structural genomics/proteomics projects provide an ideal opportunity to make such comparisons as they generate data in a systematic manner for large enough numbers of proteins to allow firm conclusions to be drawn.