[Comparison of skeletal structure of temporomandibular joint through cone-beam CT between Angle ClassⅠ, Ⅱ, Ⅱ, Ⅲ malocclusion patients with permanent dentition].

Purpose: To compare characteristics of temporomandibular joint and related structures according to gender and Angle Ⅰ, Ⅱ, Ⅱ and Ⅲ class.

Methods: Cone-beam CT (CBCT) was performed in 123 patients with malocclusion, and three-dimensional reconstruction was performed with Mimics 19.0 software.

Icariin protects vascular endothelial cells from oxidative stress through inhibiting endoplasmic reticulum stress.

Objective: To investigate the protective effect and underlying mechanism(s) of icariin (ICA) in preventing hydrogen peroxide (HO)-induced vascular endothelial cell injury via endoplasmic reticulum stress (ERS).

Methods: To study the effects of ICA on HO-induced damage, we used the cell counting kit-8 assay to detect cell viability and the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling assay to determine cell adhesion and apoptosis, respectively. Spectrophotometry and enzyme-linked immunosorbent assay were used to measure the expression levels of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px).

[Effect of cyclic tensile stress on expression of type Ⅱ collagen and Sox9 in rat cranial base synchondrosis].

Purpose: To establish an in vitro mechanical stimulation model of cranial base synchondrosis chondrocytes, and to study the effect of cyclic tensile stress on the main extracellular matrix of rat cranial base synchondrosis.

Methods: Cyclic tensile stress was imposed to the second passage of cranial base synchondrosis chondrocytes for 3, 6, 12 and 24 hours respectively by using a Flexcell Strain Unit-5000T(10% surface elongation, 1 Hz). After mechanical loading, the total RNA of the cells harvested from six-well BioFlex was extracted.

[Expression of G-protein coupled estrogen receptor in the testis of the male mouse with kidney yin or kidney yang deficiency and its impact on the reproductive function of the mouse].

Objective: To investigate the expression of the G-protein coupled estrogen receptor (GPER) in the testis of the male mouse with kidney yin or kidney yang deficiency and its influence on the reproductive function of the mouse.

Methods: We randomized 30 six-week-old male Kunming mice into three groups of equal number: kidney yang deficiency, kidney yin deficiency, and normal control, and established the models of kidney yang deficiency and kidney yin deficiency by peritoneal injection of hydrocortisone at 50 mg/kg for 5 days and 25 mg/kg for 10 days, respectively. We observed the behavioral changes of the mice using the elevated plus-maze, exhaustive swimming and field experiment, examined the semen quality with the automatic sperm quality analyzer, calculated the average number of the offspring, measured the serum testosterone (T) and estradiol (E2) levels and T/E2 ratio by Roche electrochemiluminescence assay, and determined the localization and expression of GPER in the testis by immunohistochemistry and immunofluorescence staining.

Antihypertensive drugs use and the risk of prostate cancer: a meta-analysis of 21 observational studies.

Background: Due to the lack of strong evidence to identify the relationship between antihypertensive drugs use and the risk of prostate cancer, it was needed to do a systematic review to go into the subject.

Methods: We systematically searched PubMed, Web of Science and Embase to identify studies published, through May 2015. Two evaluators independently reviewed and selected articles involving the subject.