Publications by authors named "Cheng-Min Yang"

The Hewlett Packard Enterprise-Cray EX Frontier is the world's first and fastest exascale supercomputer, hosted at the Oak Ridge Leadership Computing Facility in Tennessee, United States. Frontier is a significant electricity consumer, drawing 8-30 MW; this massive energy demand produces significant waste heat, requiring extensive cooling measures. Although harnessing this waste heat for campus heating is a sustainability goal at Oak Ridge National Laboratory (ORNL), the 30 °C-38 °C waste heat temperature poses compatibility issues with standard HVAC systems.

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In order to evaluate seed viability of Platycodon grandiflorum, Schizonepeta tenuifolia, Andrographis paniculat, Codonopsis pilosula, Scutellaria baicalensis, Leonurus japonicus, Rabdosia rubescens, stored in the medium-term gene bank of the National Medicinal Plant Gene Bank for 4 years, we tested seed germination rate of 7 species of medicinal plant and analyzed the change of significance of levels of the germination rate in pre and post store. Seed germination rates of 7 species of medicinal plants were all decreased after 4 years, and the decrease of S. tenuifolia and S.

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Article Synopsis
  • A study was conducted to compare aboveground and root traits of different strains of Scutellaria baicalensis across 14 strains using SPSS 17.0 for data analysis.
  • The results revealed significant variations in phenotypic traits, with a high F value indicating notable differences, particularly highlighting germplasm 15’s superior characteristics.
  • Correlation analysis showed most traits significantly correlated, with the fresh root weight and reed head diameter having a strong correlation, suggesting these traits could help in germplasm evaluation and breeding strategies.
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  • - The study established a suspension cell system from callus induced by stem tips of Aquilaria sinensis, using a specific growth medium (MS + NAA + 6-BA) optimal for callus induction and subculturing.
  • - After several subcultures, the developed suspension cells exhibited a characteristic "S" growth curve, with distinct phases of slow growth, logarithmic growth, and plateauing before declining in cell density.
  • - While no agarwood sesquiterpenes were found in the growing suspension cells, the presence of these compounds was noted in cells treated with MeJA, suggesting this system could be useful for studying and potentially producing agarwood sesquiterpenes through cell culture.
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The callus of Bupleurum chinense with anthers at the stage of uninucleate was induced. After several subcultures, anther calli of B. chinense were cultured at 20 MS culture mediums with different plant hormones to differentiate into plantlets.

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Objective: To isolate and identify pathogen of the seedling blight occurred in Platycodon grandiflorum.

Method: The morphological observation, rDNA ITS sequence analysis, and Koch's postulates were used to identify the isolates of the causal agent.

Result: The isolates of the causal agent was Rhizoctonia solani.

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Objectives: The etiology of myocardial ischemia/reperfusion (I/R) injury is multifactorial, but activation of the innate immune system and the resulting inflammatory response are important components of I/R injury. The aim of this study was to investigate the protective effect of a hemoglobin-based oxygen carrier (HBOC) on cold I/R heart and to explore the underlying mechanisms.

Methods: Isolated Sprague-Dawley rat hearts were perfused in the Langendorff mode.

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Ischaemia/reperfusion (I/R) injury is harmful to the cardiovascular system and is responsible for the inflammatory response, which, in turn, aggravates cardiac dysfunction. This study was designed to investigate the protective effect and potential mechanism of a haemoglobin-based oxygen carrier on cold I/R-injured hearts. Isolated Sprague-Dawley rat hearts were perfused in Langendorff mode.

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Objective: Breeding the good varieties characters of Platycodon grandiflorum.

Methods: 20 combination, derived from 2 x 10 incomplete diallel cross with 2 male sterile lines and 10 four-generation inbred lines, analysis the 13 agronomic characters.

Results: The agronomic characters of F1 has obvious heterosis.

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Article Synopsis
  • A study analyzed the contents of 21 elements in the roots of four Bupleurum L. germplasms using soil samples from seven different regions, employing ICP-MS/ICP-AES methods for accurate measurement.
  • Results indicated that the levels of potassium (K), calcium (Ca), magnesium (Mg), and sodium (Na) were primarily influenced by the specific germplasms, while iron (Fe), copper (Cu), and other trace elements were largely affected by soil types.
  • The findings suggest that different germplasms and soil compositions significantly impact the levels of medicinal compounds in Bupleurum L., providing valuable insights for selecting the optimal germplasm and soil for cultivating high-quality plants.
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A dynamic heterogeneous phase polymerization reaction is found to be efficient for controllable cross-link of hemoglobin with glutaraldehyde. The selective absorption of the immobile phase and asymmetry of protein concentration leads to narrowness of the molecular weight distribution and lowness of the average molecular weight. Using this method, 53% of hemoglobin obtained is intermolecular cross-linked with 12 molecular equivalents of glutaraldehyde.

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Hemoglobin-based blood substitute is widely studied. As the starting materials, hemoglobin (Hb) is mainly supplied from outdated human blood and animal sources. But there are many disadvantages.

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Objective: To determine the self-compatibility of Platycodon grandiflorum and the location of microspore germination on stigma.

Method: The microspore germination of self-pollination, self-plant-pollination and cross-pollination and the pollination microspore germination in and outside of stigma were observed with fluorescence microscopy.

Result: Most pollens of self-pollination, self-plant-pollination and cross-pollination can germinate on the stigma, and after 24 hours, pollen tube entered into the ovary successfully.

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Article Synopsis
  • The study aimed to gather essential data for breeding and cultivation of Platycodon grandiflorum, focusing on pollination and pollen viability.
  • Research involved field investigations and bagging techniques for pollination, using TTC solution to assess pollen vigor, which started at 81.4% and dropped to 27.6% over three days.
  • Results indicated that optimal pollination occurs 4-6 days post-petal opening, with low self-pollination rates due to mismatched maturation of reproductive parts, while hand pollination improved compatibility rates significantly.
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For pilot-scale manufacturing of hemoglobin-based oxygen carrying drugs, we should get highly pure and viral inactivated hemoglobin (Hb) at high recovery. In our method, placenta hemoglobin (PHb) solutions were purified by heating in the presence of reducing agent and deoxygenating conditions so that heat-sensitive proteins were selectively precipitated and virus was inactivated. The optimum preparative condition resulted in highly purified PHb solution (>99% pure) with approximate 90% recovery and less than 2% of MetHb content, maintained oxygen carrying capacity, residual phospholipids less than 1 ppm, free of endotoxin, bacteria, type A&B antigens and virus.

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