Publications by authors named "Cheng-Li Wu"

Four-finger threadfin, Eleutheronema tetradactylum farming in southern Taiwan has been facing disease problems caused by Streptococcus iniae since 2018. The development of a vaccine against infectious S. iniae in the cultured threadfin industry is necessary.

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Edwardsiella anguillarum, a highly virulent species within the Edwardsiella genus, causes significant mortality in milkfish farms in Taiwan. This study aimed to investigate the comparison of milkfish susceptibility, a newly identified host species in Taiwanese aquaculture, with other species Nile tilapia (Oreochromis niloticus) and Asian seabass (Lates calcarifer), to E. anguillarum, elucidating its pathogenicity across both seawater and freshwater aquaculture environments.

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Nocardiosis, caused by Nocardia seriolae, has been a prominent disease in Southeast Asian aquaculture in the last three decades. This granulomatous disease reported in various fish species is responsible for significant economic losses. This study investigated the pathogenicity of N.

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Article Synopsis
  • The study investigated disease outbreaks in farmed Chicken Grunts in Taiwan, focusing on juvenile fish that exhibited symptoms like erratic swimming and emaciation.
  • Researchers collected 50 fish from two farms, revealing significant mortality rates (19% and 24%), and identified gross lesions including cysts in various organs.
  • The pathogen identified was Kudoa lutjanus, marking the first documented outbreak of this parasite in Chicken Grunts, which poses a warning for the aquaculture industry.
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Golden pompano (Trachinotus blochii) is a carnivorous teleost cultured in the Asia-Pacific region. Fish culture in high densities and numbers results in disease outbreaks, causing huge economic losses. Here, we collected cultured golden pompanos from 2021 to 2022 and identified the pathogens isolated from the diseased fish.

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Nocardia seriolae causes chronic nocardiosis in various marine and freshwater aquatic animals; however, grouper species have rarely been investigated. This study evaluated the pathogenicity of nocardiosis following N. seriolae infection in the orange-spotted grouper Epinephelus coioides.

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The Chinese softshell turtle (CST; ) is a freshwater aquaculture species of substantial economic importance that is commercially farmed across Asia, particularly in Taiwan. Although diseases caused by the group (Bcg) pose a major threat to commercial CST farming systems, information regarding its pathogenicity and genome remains limited. Here, we investigated the pathogenicity of Bcg strains isolated in a previous study and performed whole-genome sequencing.

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Article Synopsis
  • The study focuses on pisciene nocardiosis, a disease in fish caused by Nocardia seriolae, which is problematic in South-East Asian aquaculture.
  • Researchers conducted experiments on live and killed cells of N. seriolae, observing that delipidation altered key characteristics and reduced immune resistance in fish.
  • Results showed that delipidated cells were less virulent, leading to lower mortality rates in fish and changes in immune response, demonstrating that the virulence of nocardial lipids is crucial to its pathogenicity regardless of the cell's living status.
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For the first time, Longicollum pagrosomi Yamaguti, 1935, an acanthocephalan parasite, has been characterized with morphological, histopathological and molecular detail in farmed red snapper (Lutjanus erythropterus) in Taiwan. The diseased fish showed clinical signs of anorexia, emaciation and were gasping for air at the water's surface. Gross examination revealed extensive necrosis in the intestine and even penetration at the site of parasitic attachment, resulting in a large number of parasites being lodged in the peritoneal cavity, surrounded by black hyperplastic connective tissue.

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Chinese softshell turtles (Pelodiscus sinensis) (CST) are susceptible to infections by bacteria belonging to the Bacillus cereus group (Bcg). Bcg includes several closely related species, two of which, B. cereus and B.

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The diseased cage-cultured cobia (Rachycentron canadum) displayed clinical signs, haemorrhagic eyes, dorsal darkness and gross pathological lesions, enlargement of spleen and liver. Haemorrhages were found in brain, heart and liver with cumulative mortality rates ranging from 20% to 50%. Extensive congestion in the heart, liver, spleen, kidney and brain was observed histopathologically.

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Curly wing is one of the most frequently used genetic markers in Drosophila melanogaster, but its molecular mechanism is remained unclear. Previous results have showed that physicochemical stimulation would affect the formation of the cruly wing. Our recent study found that HO could not only affect the eclosion rate of D.

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Article Synopsis
  • The study aimed to develop a PCR-based method for quantitatively detecting Pinellia ternata in Banxia decoction and to find a suitable analysis method for herbal medicines in liquid forms.
  • Researchers used PCR to target the ITS region of Pinellia ternata and assessed the results through agarose gel electrophoresis, establishing a relationship between the herb's concentration and PCR output.
  • The results demonstrated that both methods provided accurate analysis, with agarose gel electrophoresis showing a strong correlation (r = 0.9943), establishing a reliable and reproducible approach for this type of detection in traditional Chinese medicine.
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To reveal the occurrence of Ferrum in coal, seven coal samples were selected according to the different contents of ferric oxide in the coal ash, and the content of Ferric element was determined by atomic absorption spectrophotometer (AAS) using nitric acid-perchloric acid-hydrofluoric acid wet digestion. Modes of occurrence of ferrium in the seven coal samples were studied by AAS using ultrasound-assisted sequential chemical extraction experiment (SCEE). Ultrasound promoted the contact of the extraction reagent with the coal particles and enhanced the dissolution process by producing characteristic acoustic cavitations, which greatly shortened the experimental time.

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Salicyladehyde benzoylhydrazone (SBH) has three groups suitable for forming coordination bond with transition metal. The UV-vis absorption spectra of SBH and its Co(II) complexes in various media were studied by using the deconvolution method. It is found that the structure of complex in solution is different from those in solid crystals.

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