Rapid Identification of Alkaloids and Flavonoids in Fissistigma oldhamii var. longistipitatum by Ultra High-Performance Liquid Chromatography and Quadrupole Time-of-Flight Tandem Mass Spectrometry.

The herb Fissistigma oldhamii var. longistipitatum has been used for a long time in Asian folk medicine in the treatment of several diseases, including rheumatoid arthritis and other inflammatory conditions. Researchers in China and elsewhere have analyzed and characterized its chemical content.

New compounds from the stems of Merr. and their in vitro anti-inflammatory activity.

Three new compounds, 4,5,6,7-tetramethoxy-3-benzoylbenzofuran (), 4-hydroxy-3,5,6-trimethoxydihydrochalcone-2-O-β-D-glucopyranoside () and 2-hydroxy-3,4,5,6-tetramethoxyphenylethyl benzoate () along with five known flavonoids were isolated from the dichloromethane fraction of the stems of Merr.'s ethanol extracts. The compounds were obtained by chromatographic methods and the structure elucidation was completed primarily on the basis of spectroscopic analyses, all of these compounds were isolated from for the first time.

Cellular absorption of anthraquinones emodin and chrysophanol in human intestinal Caco-2 cells.

The intestinal absorption characteristics of anthraquinones emodin and chrysophanol were observed by measuring the intracellular accumulation across Caco-2 cells by the reverse-phase high performance liquid chromatography. The intracellular accumulation of chrysophanol was much greater than that of emodin, the maximum absorption of emodin and chrysophanol being 414.02+/-15.

[Reparative and therapeutic effect of rhBMP-2m on mice injured by chemotherapy].

Aim: To probe the therapeutic effect of recombinant human bone marrow morphogenetic protein-2 maturation peptide(rhBMP-2m) on mouse bone marrow injury caused by cyclophosphamide (CTX).

Methods: 18 mice were divided randomly into 3 groups, namely CTX injection group(CTX group), BMP therapy group(BMP group) and PBS control group(Control group), 6 mice each group. CTX of 200 mg/kg per mouse was intraperitonealy(IP) injected at a time to BMP group and CTX group so as to establish the experimental model of mouse bone marrow injury.