Publications by authors named "Cheng-Feng Han"

Article Synopsis
  • - The study investigates how nitric oxide (NO) enhances polysaccharide production in Nostoc flagelliforme through a process called S-nitrosylation (SNO), which modifies certain enzymes involved in this biosynthesis.
  • - It was found that certain key enzymes (G6PDH, ICDH, and UGDH) have their activity correlated with NO levels, and specific enzymes (UGDH and G6PDH) are particularly affected by SNO, as shown through various laboratory techniques.
  • - The research identifies specific sites on the enzymes that are modified by NO and proposes that this mechanism could lead to improved industrial production of polysaccharides from Nostoc flagelliforme.
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To significantly improve the polysaccharide production of , a total of 12 chemicals were evaluated for their effects on polysaccharide accumulation. The results showed that salicylic acid and jasmonic acid increased the accumulation of the polysaccharides in significantly, by more than 20%. Three polysaccharides, namely control-capsule polysaccharide, salicylic acid-capsule polysaccharide, and jasmonic acid-capsule polysaccharide, were extracted and purified from under normal, salicylic acid, and jasmonic acid culture conditions, respectively.

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Four polysaccharide fractions were isolated and purified from the culture supernatant and mycelium of Poria cocos, and differences in their immunomodulatory activity were investigated. The average molecular weights of EPS-0M, EPS-0.1M, IPS-0M, and IPS-0.

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Two capsular polysaccharides (WL-CPS-1 and GLU-CPS-1) purified from Nostoc flagelliforme under normal and mixotrophic culture conditions were used to investigate the hypolipidemic activity and effect on intestinal flora in C57BL/6J mice respectively. Their molecular weight and monosaccharide composition have been determined in previous studies. They both improved the lipid level by affecting the expression of lipid metabolism genes.

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A strategy by exogenous addition of quorum sensing molecule farnesol to improve the production, antioxidant activity and antitumor activity of extracellular polysaccharide (EPS) of Grifola frondosa by liquid fermentation was proposed in the study. The highest yield of EPS induced by farnesol was 1.25 g/L, which was 150% higher than that of the control.

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