[Relationship between gene polymorphisms in MMP-9 and Helicobacter pylori-related upper gastrointestinal disease in children].

Objective: To investigate the relationship of the promoter of matrix metalloproteinase-9 (MMP-9) gene polymorphisms with the susceptibility and clinical features of Helicobacter pylori (H. pylori)-related chronic gastritis and duodenal ulcer in children.

Methods: One hundred children with chronic gastritis, 32 children with duodenal ulcer and 102 healthy children were enrolled.

Does the geographical gradient of ApoE4 allele exist in China? A systemic comparison among multiple Chinese populations.

The allelic frequencies of apolipoprotein E (apoE) vary substantially around the world. There is a conspicuous south-to-north gradient of e4 frequencies in Europe, with the proportion of e4 carriers from only 10-15% in the south to 40-50% in the north. The mechanism may be related to the possibility that e4 carriers are less likely to develop vitamin D deficiency.

Effect of apolipoprotein B polymorphism on body mass index, serum protein and lipid profiles in children of Guangxi, China.

Background: Some studies have demonstrated that genetic variance of apolipoprotein B (APOB) plays an important role in lipid metabolism in childhood. However, little information exists about the distribution and effect of the APOB polymorphism among the population from Guangxi, where populations have developed through complex interaction among various ethnic groups over the centuries. The purpose of this study was to investigate the association of genetic polymorphisms at the APOB XbaI, EcoRI and MspI restriction sites with body mass index (BMI), serum protein and lipid profiles in children of Guangxi.

Genetic variation of apolipoprotein E does not contribute to the lipid abnormalities secondary to childhood minimal change nephrotic syndrome.

Minimal change nephrotic syndrome (MCNS) is a common progressive renal disorder occurring in childhood that is characterized by alterations of permselectivity at the glomerular capillary wall, resulting in its inability to restrict the urinary loss of protein. Hyperlipidemia (HLP) is not only an important clinical manifestation of MCNS but is also involved in cardiovascular disease and in progressive renal damage. ApoE is a polymorphic protein.

Association of polymorphisms at restriction enzyme recognition sites of apolipoprotein B and E gene with dyslipidemia in children undergoing primary nephrotic syndrome.

Background: Dyslipidemia, a common complication, is very prevalent in children with primary nephrotic syndrome (PNS). Recent studies have shown that genetic basis may be involved in the onset of HLP secondary to PNS. ApoB and E have been identified as the important candidate genes for lipid abnormalities.

[Rapid diagnosis of 21 trisomy syndrome by fluorescence quantitative polymerase chain reaction].

Objective: To establish a method of fluorescence quantitative PCR to detect 21 trisomy syndrome.

Methods: At first, using one pair of primer to simultaneously amplify different fragments of two highly homologous genes of the human liver-type phosphofructokinase located on chromosome 21 (PFKL-CH21) and the human muscle-type phosphofructokinase located on chromosome 1 (PFKM-CH1). Then, staining the PCR products of these homologous genes with SYBR Green I, comparing the fluorescence intensities of the bands after electrophoresis, and analyzing the data.