Publications by authors named "Chenbo Huang"

Culm strength is crucial for rice growth, nutrition transportation, and structural resilience, which are essential for lodging resistance and stable production. In this study, we identified a rice thin culm mutant tc4, characterized by thinner culms and thicker cavity walls, resulting in weakened culm mechainical strength. Using map-based cloning, the candidate gene was isolated, and complementation and CRISPR/Cas9 experiments confirmed that a single nucleotide substitution in TC4 is responsible for the thin and brittle culm phenotype.

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Article Synopsis
  • The flowering period of rice is crucial for its adaptability and yield, and can be optimized through genome editing techniques like CRISPR/Cas9.
  • A new multiplex genome-editing vector targeting five flowering-related genes was developed and introduced into the Jiahe212 rice variety, resulting in mutant plants with varied flowering times.
  • Three specific mutant lines flowered earlier without significantly affecting yield, providing valuable resources for future rice breeding efforts aimed at improving adaptability and creating hybrids.
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Formation of the pollen wall, which is mainly composed of lipid substances secreted by tapetal cells, is important to ensure pollen development in rice. Although several regulatory factors related to lipid biosynthesis during pollen wall formation have been identified in rice, the molecular mechanisms controlling lipid biosynthesis are unclear. In this study, we isolated the male-sterile rice mutant oslddt1 (leaked and delayed degraded tapetum 1).

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