Publications by authors named "Chenbo Gong"
Improvement of Rice Agronomic Traits by Editing Type-B Response Regulators.
Int J Mol Sci
November 2022
Type-B response regulator proteins in rice contain a conserved receiver domain, followed by a GARP DNA binding domain and a longer C-terminus. Some type-B response regulators such as RR21, RR22 and RR23 are involved in the development of rice leaf, root, flower and trichome. In this study, to evaluate the application potential of type-B response regulators in rice genetic improvement, thirteen type-B response regulator genes in rice were respectively knocked out by using CRISPR/Cas9 genome editing technology.
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- The study reports the creation of gap-free reference genome sequences for two elite rice varieties, Zhenshan 97 and Minghui 63, enhancing our understanding of rice genomics.
- These genomes facilitate a comprehensive analysis of centromere structure, revealing shared satellite motifs and evidence of local expansion of CentO repeats.
- The research also identifies non-transposable element genes in centromeres and structural variants affecting resistance genes, paving the way for improved plant breeding and climate resilience.
Article Synopsis
- - The study investigates the role of the OsPPR16 protein in rice, which is part of the PLS-DYW subfamily of pentatricopeptide repeat proteins that edits chloroplast rpoB mRNA, crucial for proper plastid transcription.
- - Using the CRISPR/Cas9 technique, researchers knocked out OsPPR16, leading to reduced levels of RpoB and PEP-dependent genes, resulting in a pale phenotype during early development.
- - The findings highlight the importance of OsPPR16 in regulating plastid gene expression and its role in chlorophyll synthesis and chloroplast development.