Development and psychometric properties of the nursing ethical decision-making ability scale.

Background: Nursing ethical decision-making ability is a core competency of nurses. However, no tool has been developed to measure the ethical decision-making ability of nurses in China. Therefore, we aimed to develop a nursing ethical decision-making ability scale (EDMAS) and assess its validity and reliability.

The Redox Cycle of Viologen-Based Porous Organic Polymers for High-Performance Hydrogen Peroxide Photosynthesis.

Harnessing solar energy to produce hydrogen peroxide (H2O2) is an important strategy to address the current energy shortage. Among various materials, porous organic polymers (POPs) stand out as promising candidates for photocatalytic H2O2 generation due to their versatile structures and extended π-conjugation; however, their efficiency in H2O2 synthesis is often constrained by poor charge separation and high reaction energy barriers. To address these challenges, we drew inspiration from the redox reactions of coenzyme NADPH in natural photosynthesis, the redox-active methyl viologen (MV2+) moiety was rationally incorporated into two polymer frameworks (TPE-MV and TPB-MV) as pivotal active sites for photocatalytic oxygen (O2) reduction to H2O2 via the reversible redox cycle of MV2+ and zerovalent methyl viologen (MV0).

Highly Diastereoselective Synthesis of 5/6-Fused Bicyclic Ring Systems via Radical Cyano Group Migration.

Here we report a highly diastereoselective cyano group transfer radical cyclization reaction to construct 5/6-fused bicyclic ring systems that bear three contiguous and congested stereogenic elements, with 100% atom economy under catalyst-free and near-ultraviolet light irradiation conditions. Mechanistic investigations and density functional theory calculations suggest that the diastereoselectivity is governed by the conformational distribution of the triplet diradical intermediate and the rate of reverse intersystem crossing (RISC) before radical coupling.

Rootstock Selection for Resisting Cucumber Wilt in Hainan and Corresponding Transcriptome and Metabolome Analysis.

Soilborne diseases are important problems in modern agricultural production. f. sp.

Innovative nanoparticle-based approaches for modulating neutrophil extracellular traps in diseases: from mechanisms to therapeutics.

Neutrophil extracellular traps (NETs) participate in both host defense and the pathogenesis of various diseases, such as infections, thrombosis, and tumors. While they help capture and eliminate pathogens, NETs' excessive or dysregulated formation can lead to tissue damage and disease progression. Therapeutic strategies targeting NET modulation have shown potential, but challenges remain, particularly in achieving precise drug delivery and maintaining drug stability.

RNA-seq reveals Lysyl oxidase as a potential biomarker of glomerular function in diabetic nephropathy in rats.

Purpose: We downloaded the gene expression profiles of patients with diabetic nephropathyfrom the GEO database and combined it with differential gene analysis of rat transcriptome,our study employed animal models to examine the role of key hub genes in diabetic nephropathy and to pinpoint significant gene regulation in this disease.

Methods: An examination of differential expression was performed using the online analysis tool GEO2R and the DN-related datasets GSE30528 and GSE1009 obtained from the GEO database. A comparison of gene expression between the normal and diabetic nephropathy groups was conducted using the RNA-seq technique.

Neo-BCV: A Novel Bacterial Liquid Complex Vaccine for Enhancing Dendritic Cell-Mediated Immune Responses Against Lung Cancer.

Background: In the past decade, immunotherapy has become a major choice for the treatment of lung cancer, yet its therapeutic efficacy is still relatively limited due to the various immune escape mechanisms of tumors. Based on this, we introduce Neo-BCV, a novel bacterial composite vaccine designed to enhance immune responses against lung cancer.

Methods: We investigated the immune enhancing effect of Neo-BCV through in vivo and in vitro experiments, including flow cytometry, RNA-seq, and Western blot.

Leaf Photosynthetic and Respiratory Thermal Acclimation in Terrestrial Plants in Response to Warming: A Global Synthesis.

Leaf photosynthesis and respiration are two of the largest carbon fluxes between the atmosphere and biosphere. Although experiments examining the warming effects on photosynthetic and respiratory thermal acclimation have been widely conducted, the sensitivity of various ecosystem and vegetation types to warming remains uncertain. Here we conducted a meta-analysis on experimental observations of thermal acclimation worldwide.

Neutrophil Extracellular Trap Formation Model Induced by Monosodium Urate and Phorbol Myristate Acetate: Involvement in MAPK Signaling Pathways.

Neutrophil extracellular traps (NETs) formation is a key process in inflammatory diseases like gout, but the underlying molecular mechanisms remain incompletely understood. This study aimed to establish a model to examine the formation of NETs induced by monosodium urate (MSU) and phorbol 12-myristate 13-acetate (PMA) and to elucidate their molecular pathways. Laser confocal microscopy was used to visualize NET formation, while flow cytometry was employed to detect reactive oxygen species (ROS) production.

Machine learning derivation of two cardiac arrest subphenotypes with distinct responses to treatment.

Introduction: Cardiac arrest (CA), characterized by its heterogeneity, poses challenges in patient management. This study aimed to identify clinical subphenotypes in CA patients to aid in patient classification, prognosis assessment, and treatment decision-making.

Methods: For this study, comprehensive data were extracted from the Medical Information Mart for Intensive Care IV (MIMIC-IV) 2.

Inferring murine cerebrospinal fluid flow using artificial intelligence velocimetry with moving boundaries and uncertainty quantification.

Cerebrospinal fluid (CSF) flow is crucial for clearing metabolic waste from the brain, a process whose dysregulation is linked to neurodegenerative diseases like Alzheimer's. Traditional approaches like particle tracking velocimetry (PTV) are limited by their reliance on single-plane two-dimensional measurements, which fail to capture the complex dynamics of CSF flow fully. To overcome these limitations, we employ artificial intelligence velocimetry (AIV) to reconstruct three-dimensional velocities, infer pressure and wall shear stress and quantify flow rates.

Longitudinal Evaluation of FDG Uptake in a Synovial Herniation Pit in 8 Years: Implications for Potential False-Positive PET Findings.

Synovial herniation pit, also known as Pitt's pit, is a benign osseous lesion incidentally seen on imaging studies. Most of them will not show FDG uptake, but some herniation pits could show intense FDG uptake on PET/CT scans mimicking osseous metastasis. Here we report a case of synovial herniation pit in a patient with T-cell lymphoma through 8-year trajectory of FDG PET/CT scans, revealing its changing FDG uptake in relation to chemotherapy and final outcome.

Biofilm formation on microplastics and interactions with antibiotics, antibiotic resistance genes and pathogens in aquatic environment.

Microplastics (MPs) in aquatic environments easily support biofilm development, which can interact with other environmental pollutants and act as harbors for microorganisms. Recently, numerous studies have investigated the fate and behavior of MP biofilms in aquatic environments, highlighting their roles in the spread of pathogens and antibiotic resistance genes (ARGs) to aquatic organisms and new habitats. The prevalence and effects of MP biofilms in aquatic environments have been extensively investigated in recent decades, and their behaviors in aquatic environments need to be synthesized systematically with updated information.

Kaempferol attenuates hyperuricemia combined with gouty arthritis via urate transporters and NLRP3/NF-κB pathway modulation.

Hyperuricemia (HUA), caused by purine disorders, can lead to gouty arthritis (GA). Kaempferol (KPF), a natural flavonoid, has anti-inflammatory properties, though its mechanism in treating HUA combined with GA remains unclear. This study used a mouse model of HUA combined with GA and models with HK-2 and THP-1 cells to explore KPF's effects.

The accumulation and inhibition mechanism of extracellular polymeric substances of Chlorella vulgaris during cycling cultivation under different light qualities.

The secretion, accumulation, and composition of extracellular polymeric substances (EPS) are pivotal factors influencing microalgal growth as well as wastewater recycling. Until now, the accumulation and inhibition mechanism of EPS of Chlorella vulgaris during cycling cultivation is not fully understood. The purpose of this study was to explore how different light qualities regulate the secretion, chemical composition, and structure of microalgal EPS, and subsequently influence the recycling of culture wastewater.

Phosphorus cycle in shallow lakes affected by crucian carp (Carassius auratus): Effects of fish density and size.

Crucian carp (Carassius auratus) is an omni-benthivorous fish common in many shallow lakes in China. The presence of crucian carp can contribute to the nutrient cycles in lakes and thus affect water quality. In this work, a two-by-two factorial mesocosm experiment was performed with crucian carp of different sizes and densities, to investigate their effects on the cycle of phosphorus (P).

Kemin capsule ameliorates post-infectious cough by modulating the PI3K/AKT signaling pathway and TRPA1/TRPV1 channels.

Ethnopharmacological Relevance: Kemin capsule (KMC), as an innovative traditional Chinese medicine (TCM), has shown excellent efficacy in treating PIC in China. The post-infectious cough (PIC) is a common condition in pediatrics, and the inflammatory responses to PIC are intricately linked to the immune mechanisms of the host. However, the precise mechanisms involved remain uncertain.

Impact of predictive selection of LbCas12a CRISPR RNAs upon on- and off-target editing rates in soybean.

Clustered regularly interspaced short palindromic repeats (CRISPR) technology has revolutionized creating targeted genetic variation in crops. Although CRISPR enzymes have been reported to have high sequence-specificity, careful design of the editing reagents can also reduce unintended edits at highly homologous sites. This work details the first large-scale study of the heritability of on-target edits and the rate of edits at off-target sites in soybean (), assaying ~700 T1 plants each resulting from transformation with LbCas12a constructs containing CRISPR RNAs (crRNAs) predicted to be either "unique" with no off-target sites or "promiscuous" with >10 potential off-targets in the soybean genome.

Optimizing young tennis players' development: Exploring the impact of emerging technologies on training effectiveness and technical skills acquisition.

The research analyzed the effect of weekly training plans, physical training frequency, AI-powered coaching systems, virtual reality (VR) training environments, wearable sensors on developing technical tennis skills, with and personalized learning as a mediator. It adopted a quantitative survey method, using primary data from 374 young tennis players. The model fitness was evaluated using confirmatory factor analysis (CFA), while the hypotheses were evaluated using structural equation modeling (SEM).