CM3-SII polysaccharide obtained from Cordyceps militaris ameliorates hyperlipidemia in heterozygous LDLR-deficient hamsters by modulating gut microbiota and NPC1L1 and PPARα levels.

Accumulating evidence has demonstrated that polysaccharides derived from edible fungi have lipid-lowering effects in mice. However, the lipid metabolism mechanisms in mice and humans are different. We have previously elucidated the structural characteristics of the alkali-extracted polysaccharide CM3-SII obtained from Cordyceps militaris.

Targeting the PDGF/PDGFR signaling pathway for cancer therapy: A review.

Platelet-derived growth factors (PDGFs) and PDGF receptors (PDGFRs) are expressed in a variety of tumors. Activation of the PDGF/PDGFR signaling pathway is associated with cancer proliferation, metastasis, invasion, and angiogenesis through modulating multiple downstream pathways, including phosphatidylinositol 3 kinase/protein kinase B pathway and mitogen-activated protein kinase/extracellular signal-regulated kinase pathway. Therefore, targeting PDGF/PDGFR signaling pathway has been demonstrated to be an effective strategy for cancer therapy, and accordingly, some great progress has been made in this field in the past few decades.

Improving Clinician-Patient Communication Alleviates Stigma in Patients With Functional Dyspepsia Receiving Antidepressant Treatment.

Background/aims: Antidepressants are effective in patients with functional dyspepsia (FD). However, stigma associated with FD and antidepressants may affect treatment adherence. This study aims to explore possible communication strategies to alleviate stigma and improve adherence in patients with FD.

Identification of macrophage related gene in colorectal cancer patients and their functional roles.

Background: Recent scientific research has enabled the identification of macrophages related-genes (MaRG), which play a key role in the control of the immune microenvironment in many human cancers. However, the functional role of MaRGs in human tumors is ill-defined. Herein, we aimed at bioinformatically exploring the molecular signatures of MaRGs in colorectal cancer.

The impact of stigma on medication adherence in patients with functional dyspepsia.

Background: Psychological factors contribute to the pathogenesis of functional dyspepsia (FD). Antidepressant agents are beneficial in treatment of refractory FD. However, their efficacy is greatly hindered by the poor treatment adherence.

Analysis of pathogenetic process of fungal rhinosinusitis: Report of two cases.

Background: Fungal rhinosinusitis is an infectious and/or allergic disease caused by fungi in the sinus and nasal cavity. Due to the warm and humid climate in Guangxi Zhuang Autonomous Region, the incidence of fungal rhinosinusitis is higher than that in other provinces. However, its physiological mechanism is not yet clear.

Species classification and bioactive ingredients accumulation of BaiJiangCao based on characteristic inorganic elements analysis by inductively coupled plasma-mass spectrometry and multivariate analysis.

Background: Patrinia scabiosaefolia Fisch and Patrinia villosa (Thunb.) Juss., two species herbs with the same Chinese name "BaiJiangCao", are important ancient herbal medicines widely used for more than 2000 years.

Apoptosis of human gastric cancer SGC-7901 cells induced by podophyllotoxin.

Numerous studies have demonstrated that podophyllotoxin and its derivatives exhibit antitumor effects. The aim of the present study was to investigate SGC-7901 cell apoptosis and the underlying mechanism induced by podophyllotoxin. SGC-7901 cells were treated with varying concentrations of podophyllotoxin.

Laminarin-induced apoptosis in human colon cancer LoVo cells.

A number of scientific studies have revealed that laminarin has antitumor effects. Therefore, the aim of the present study was to investigate the apoptosis of LoVo cells and the underlying mechanisms induced by laminarin. LoVo cells were treated with various concentrations of laminarin and fluorescence-inverted microscopy was used to observe the morphology of LoVo cells treated with laminarin.

Sulfated modification and anti-tumor activity of laminarin.

The aim of this study was to investigate the sulfated modification of laminarin and the changes in structure and antitumor activity. The chlorosulfonic acid-pyridine method was applied for sulfated modification. The molecular weights of laminarin and laminarin sulfate (LAMS) were measured by high-performance liquid chromatography (HPLC), and IR and NMR spectra were also recorded.

Human gastric cancer cell line SGC-7901 apoptosis induced by SFPS-B2 via a mitochondrial-mediated pathway.

This study was to investigate the effect of Sargassum fusiforme polysaccharides (SFPS-B2) on the proliferation and apoptosis of human gastric cancer cell line SGC-7901. Cells were treated with different concentrations of SFPS-B2. MTT and flow cytometry (FCM) assays were performed to evaluate the effect of SFPS-B2 on the cell growth and apoptosis.

Study on human promyelocytic leukemia HL-60 cells apoptosis induced by fucosterol.

In this study, we investigated the effect of fucosterol on HL-60 and the molecular mechanism. HL-60 Cells were treated with fucosterol, and 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) method was used to study fucosterol anti-tumor activity. Morphology of HL-60 cells was observed.

Laminarin induces apoptosis of human colon cancer LOVO cells through a mitochondrial pathway.

Many scientific studies have shown that laminarin has anti-tumor effects, but the anti-tumor mechanism was unclear. The purpose of this study was to investigate the effect of laminarin on the induction of apoptosis in human colon cancer LOVO cells and the molecular mechanism involved. LOVO cells were treated with different concentrations of laminarin at different times.

[Determination and analysis of toluene diisocyanate metabolites in mice using gas chromatography-mass spectrometry].

In the present research we used gas chromatography-mass spectrometry (G C-MS) to determine metabolites of toluene diisocyanate (TDI) in mice and deduce the pathway for toluene diisocyanate metabolism in the organism. Conditions for TDI chromatography: Supelco PTETM-5 chromatographic column (30 mm x 0.25 mm x 0.

[Effect of Sudan I, III and IV on proliferation of HepG-2 and SGC-7901].

To study the effect of sudan I, III and IV on the growth of HepG-2 and SGC-7901, the ratio of DNA/RNA and 3D image in HepG-2 treated by different dose of sudan I, III and IV was detected by LCM, the content change of DNA and the cell cycle in SGC-7901 treated by different dose of sudan I, III and IV was detected by flow cytometry. Results showed the ratio of DNA/RNA in control group was 1.2232 +/- 0.

[Study on the effects of two kinds of cactus polysaccharide on erythrocyte immune function of S180 mice].

Objective: To study the effects of two kinds of cactus polysaccharide on erythrocyte immune function in S180 mice.

Method: Classical pharmaceutical method and test kit.

Result: The cactus polysaccharide increased the content of RBC-CaR, RFER, decreased the content of RFIR, raised the content of sialic acid.

[Study on the effects of cool and hot ethanol extracts from qinglongyi on the biochemical function of tumor membrane in H22 mice].

Objective: To study the effects of cool and hot ethanol extracts from Qinglongyi on tumor membrane protein content, lipid fluidity, and membrane close capability in H22 mice.

Method: The membrane protein content, by, lipid fluidity and membrane close capability were measured by means of SDS-PAGE, skinitzky assay and Zamudio method respectively.

Result: The cool and hot ethanol extracts from Qinglongyi decreased the content of tumor membrane protein, the lipid fluidity and membrane close capability.

[Study on the effects of two kinds of cactus polysaccharide on erythrocyte membrane protein and fluidity of the lipid in S180 mice].

Objective: To study the effects of two kinds of cactus polysaccharide on Band 3 protein, cross-linking protein and lipid fluidity of erythrocyte membrane in S180 mice.

Method: The membrane protein content was analysed by SDS-PAGE. Lipid fluidity was measured by Skinitzky method.