Characterization of the pH-dependent protein stability of 3α-hydroxysteroid dehydrogenase/carbonyl reductase by differential scanning fluorimetry.

The characterization of protein stability is essential for understanding the functions of proteins. Hydroxysteroid dehydrogenase is involved in the biosynthesis of steroid hormones and the detoxification of xenobiotic carbonyl compounds. However, the stability of hydroxysteroid dehydrogenases has not yet been characterized in detail.

[Structural Design and Finite Element Analysis of New Type Posterior Integral Glaucoma Valve].

The treatment of refractory Glaucoma is a difficult problem in clinical ophthalmology. For refractory glaucoma patients with hyphema, shallow anterior chamber, anterior conglutination of peripheral chamber angle, corneal endothelium dystrophy or decompensated, at present, there is no effective treatment. In order to solve this problem, a new type posterior integral glaucoma valve with IOP control device was designed using medical titanium alloy, and the valve model was established by Abaqus software, and the stiffness and preload of the valve were analyzed by finite element method.

Nematotoxic coumarins from Maxim. Shan Yuan roots and their physiological effects on .

The ethanol extracts from the roots of Maxim. Shan Yuan was toxic against the pine wood nematode The ethyl acetate-soluble fraction derived from this extract increased its potency with a mortality of 95.25% in 72 hr at 1.

[Chemical constituents from Embelia laeta].

Objective: To study the chemical constituents from Embelia laeta.

Methods: The constituents from the EtOAc fraction of ethanol extract of Embelia laeta were separated and purified by column chromatography with silica gel and polyamide. The compounds were identified by their physiochemical proerties and spectral data.

[Studies on chemical constituents in n-butyl alcohol extract of roots of Actinidia deliciosa in Guangxi].

Objective: To study the chemical constituents of n-butyl alcohol extract in the roots of Actinidia deliciosa in Guangxi.

Method: The constituents were separated with various chromatographic techniques and their structures were elucidated by means of physicochemical properties and the analysis of their spectral data.

Result: Six compounds were isolated and identified as eriantic acid B (1), 2alpha, 3beta, 24-trihydroxyursa-12-en-28-oic acid (2), 2alpha, 3alpha, 24-trihydroxyursa-12-en-28-oic acid (3), 2alpha, 3alpha, 23-tri-hydroxyursa-12, 20 (30)-dien-28-oic acid (4), 2alpha, 3alpha, 24-trihydroxyursa-12, 20 (30)-dien-28-oic acid (5), n-butyl-O-beta-D-fruto-pyranoside (6).

[Studies on the chemical constituents of the ethyl acetate portion of Nervilia fordii].

Objective: To study the chemical constituents of the ethyl acetate portion in the herb of Nervilia fordii from guangxi.

Methods: The constituents were separated and purified by using column chromatography with silica gel. These compounds were identified by their physical and spectral data.