Publications by authors named "Chen Zuoliang"
Objective: The aim of this study was to examine the antitumor effects of Qilan preparation on oral squamous cell carcinoma (OSCC) and to investigate its underlying mechanisms of action.
Methods: Cell proliferation, cell cycle distribution and apoptosis were examined using cell counting kit-8 (CCK8) and flow cytometry (FCM). The expression of PTEN and PDCD4 were determined by western blot.
Purpose: To study the cancer blocking effect of the Qi-lan granulates in SD rats.
Methods: A total of 150 SD rats were divided into five groups A,B,C,D,E. Rats in group A, B, C, D were fed with 0.
[Significance of cell immunoreactions and cell apoptosis in oral lichen planus].
Hua Xi Kou Qiang Yi Xue Za Zhi
June 2009
Objective: To evaluate the expression of CD4+, CD8+ T cells and cell apoptosis in oral lichen planus (OLP) and investigate the role and the relationship of immunological reaction and cell apoptosis in the pathogenesis of OLP2.
Methods: Immunohistochemical technique was used to study the expression of CD4+, CD8+ T cells in 27 OLP cases. TUNEL was used for detecting the cell apoptotic index (AI) in 17 OLP2 cases.
[Expression of survivin, Bcl-2 and p53 during 4-nitro-quinoline 1-oxide-induced rat tongue carcinogenesis].
Zhonghua Kou Qiang Yi Xue Za Zhi
March 2009
Objective: To investigate the expression of survivin during oral carcinogenesis and its relationship with the expression of Bcl-2 and p53.
Methods: Two-step immunohistochemical method was employed to detect the expression of survivin, Bcl-2 and p53 in 60 rat tongue carcinogenesis specimens induced by 4-nitro-quinoline 1-oxide (4NQO).
Results: Survivin was present in 1/36 cases of normal mucosa, 6/11 cases of oral epithelial dysplasia and 10/11 cases of oral carcinoma.
[Expression of TGF-beta1, Smad7 and cell apoptosis in epithelium of oral lichen planus].
Zhonghua Kou Qiang Yi Xue Za Zhi
February 2008
Objective: To examine the expression of TGF-beta1, Smad7 and cell apoptosis in oral lichen planus (OLP) and to evaluate the possible pathogenesis of oral lichen planus.
Methods: Immunohistochemical technique was used to study the expression of TGF-beta1 and Smad7 in the epithelia cells of 17 OLP cases and 7 normal oral mucosa (NOM). TUNEL was used for detecting the cell apoptosis in 17 OLP cases and 7 NOM.
Purpose: The purpose of this study is to develop a novel root canal filling sealers based on calcium phosphate cement (CPC), and to evaluate its physical-chemical properties and in vitro antibacterial activity on the predominant bacteria infecting root canal.
Methods: The fluidity and the setting time of the sealer were tested according to ISO 6876:2001(E) standards. The crystal size of the final product was determined.
Objective: The purpose of this study was to in situ characterize the demineralization (namely dissolution of hydroxyapatite) on the surface of the human enamel using electrochemical impedance spectroscopy (EIS) technology.
Methods: Fresh human third molars extracted without visible evidence of caries, were used in this study. After they were immersed in a demineralizing solution prepared from lactic acid and carboxy methyl cellulose sodium (Na-CMC) buffering at pH 4, demineralization happened on their surfaces.
Objective: To study the effect of Chinese herbal medicine 1023 Recipe in blocking cancer transformation of experimental oral precancerous lesion and its mechanism.
Methods: We treated the experimental oral precancerous lesion in hamster's cheek pouch using 1023 Recipe (consisting of Radix Astragali, Gynostemma Pentaphyllum, Rhizoma Chuanxiong and selenium-rich green tea) for 6 weeks, and observed its effect in blocking cancer transformation, detected 2 kinds of agglutinin receptors (receptors of wheat germ agglutinin and Ricinus communis agglutinin) in the mucosa of the hamster's cheek pouch.
Results: The rate of cancer transformation in 1023 Recipe treated group was lower than that in the control group without treatment (P<0.