[Effect of glutamine on intestinal mucosal barrier function in rats with acute hepatic injury].

Objective: To explore the role of glutamine in LPS and D-Gal induced acute hepatic injury.

Methods: A total of 61 Wistar rats were randomly divided into three groups: control group, model group and GLN pretreated group. The animal model was established by LPS and D-Gal intraperitoneal injection.

[Establishment of a D-galactosamine/lipopolysaccharide induced acute-on-chronic liver failure model in rats].

Objective: To establish a practical and reproducible animal model of human acute-on-chronic liver failure for further study of the pathophysiological mechanism of acute-on-chronic liver failure and for drug screening and evaluation in its treatment.

Methods: Immunological hepatic fibrosis was induced by human serum albumin in Wistar rats. In rats with early-stage cirrhosis (fibrosis stage IV), D-galactosamine and lipopolysaccharide were administered.

Influence of HBcAg in liver cell plasma on expression of transforming growth factor-beta 1 in liver tissue of low-grade chronic hepatitis B patients.

Aim: To study the influence of HBcAg on the expression of transforming growth factor-beta 1 (TGF-beta1) in liver tissue of low-grade chronic hepatitis B (CHB) patients.

Methods: The expression of TGF-beta1 and HBcAg in liver samples from 93 low-grade CHB patients was detected by immunohistochemistry and valuated by semi-quantitative scoring.

Results: In the 93 low-grade CHB patients, HBcAg was expressed in cell plasma but not in the liver tissue.

A clinicopathological study of three cases of severe acute respiratory syndrome (SARS).

Aims: The severe acute respiratory syndrome (SARS) caused a large outbreak of atypical pneumonia in Beijing, China from early March 2003. We report the pathological features from three patients who died of SARS.

Methods: Autopsies were performed on three patients who died 9-15 days after the onset of the illness, and the clinical and laboratory features reviewed.

[Detection of hepatic progenitor cells in patients with severe hepatitis and their distribution].

Objectives: To identify hepatic progenitor cells (HPCs) in patients with severe hepatitis (SH) by detecting their markers and investigate the features of their distribution and location.

Methods: Liver tissues taken from 59 SH patients were tested for the receptor of stem cell factor (c-kit), pi-class glutathione S-transferase (GST-pi), cluster of differentiation 34 (CD34), cytokeratin 19 (CK19), cytokeratin 18 (CK18) and alpha fetoprotein (AFP) by immunohistochemistry (IHC). Meanwhile, 58 patients with acute or chronic hepatitis were also detected to act as controls.

[A clinicopathological study on 3 cases of severe acute respiratory syndrome].

Objective: To study the pathological characteristics of severe acute respiratory syndrome (SARS) and its relationship to clinical manifestation.

Methods: Tissue specimens from 3 autopsy cases of diagnosed SARS were studied under microscopy and the clinical data were reviewed.

Results: The typical pathological changes of lungs were diffuse hemorrhage on surface.

[A clinicopathological study on nonalcoholic steatohepatitis].

Objective: To observe the pathological and clinical characters of nonalcoholic steatohepatitis (NASH).

Methods: Liver biopsy tissues taken from 97 patients negative for common viral detection with serological test and immunohistochemistry as well as in situ hybridization, were observed by routine light microscopic examination. And the clinical data of patients with NASH was analyzed.