Antifungal Resistance Patterns of Microsporum canis: A 27-Year MIC Study in Mainland China.

Background: Microsporum canis, a dermatophyte commonly associated with pets, is a leading cause of severe tinea capitis. The increasing prevalence of antifungal resistance among dermatophytes poses a significant global health challenge.

Objectives: This study aims to define the updated antifungal susceptibility profile of M.

One-pot isothermal CRISPR/Dx system for specific and sensitive detection of microRNA.

MicroRNA (miRNA) is a promising biomarker for the early diagnosis of pancreatic cancer. To enable sensitive and reliable miRNA detection, we have developed a one-pot isothermal CRISPR/Dx detection system by combining rolling circle amplification (RCA) and CRISPR/Cas12a. RCA and CRISPR/Cas12a reactions are carried out in a single closed tube, bypassing the transferring step.

RNF123 inhibits cell viability, cell cycle and colony formation of breast cancer by inhibiting glycolysis via ubiquitination of PFKP.

E3 ubiquitin ligases have the potential to modulate key oncogenic pathways. RING finger protein 123 (RNF123), as an E3 ubiquitin ligase, has been functioned as a tumor suppressor. This study was designed to explore the role of RNF123 in breast cancer.

BSDA: Bayesian Random Semantic Data Augmentation for Medical Image Classification.

Data augmentation is a crucial regularization technique for deep neural networks, particularly in medical imaging tasks with limited data. Deep learning models are highly effective at linearizing features, enabling the alteration of feature semantics through the shifting of latent space representations-an approach known as semantic data augmentation (SDA). The paradigm of SDA involves shifting features in a specified direction.

Cell Sheets Formation Enhances Therapeutic Effects of Human Umbilical Cord Mesenchymal Stem Cells on Spinal Cord Injury.

Background: In recent years, the utilization of stem cell therapy and cell sheet technology has emerged as a promising approach for addressing spinal cord injury (SCI). However, the most appropriate cell type and mechanism of action remain unclear at this time. This study sought to develop an SCI rat model and evaluate the therapeutic effects of human umbilical cord mesenchymal stem cell (hUC-MSC) sheets in this model.

College students' psychology and behavior in the context of online public opinion: a cross-sectional study in Jiangsu Province, China.

Background: With the rapid development of the Internet and the widespread use of social media, online public opinion has profoundly impacted the psychology and behavior of college students. College students are in a crucial stage of psychological development and self-awareness, making them highly sensitive to online information and easily influenced by online public opinion.

Methods: This study employed a cross-sectional design to explore the psychological adaptation and behavioral responses of college students to online public opinion.

A review focuses on a neglected and controversial component of SCI: myelin debris.

Myelin sheath, as the multilayer dense structure enclosing axons in humans and other higher organisms, may rupture due to various injury factors after spinal cord injury, thus producing myelin debris. The myelin debris contains a variety of myelin-associated inhibitors (MAIs) and lipid, all inhibiting the repair after spinal cord injury. Through summary and analysis, the present authors found that the inhibition of myelin debris can be mainly divided into two categories: firstly, the direct inhibition mediated by MAIs; secondly, the indirect inhibition mediated by lipid such as cholesterol.

Efficacy and safety of minocycline in retinitis pigmentosa: a prospective, open-label, single-arm trial.

Retinitis pigmentosa (RP) is characterized by progressive photoreceptor cells death accelerated by the proliferation and activation of microglia pathologically. No consensus exists on the treatment. Minocycline is recognized as a microglia inhibitor with great anti-inflammatory and neuro-protective functions.

Effect of Hyperbaric Oxygen Therapy on Patients with SARS-CoV-2 Infection: A Retrospective Cohort Study.

Objective: The aim of this study was to evaluate the impact of hyperbaric oxygen therapy (HBOT) on patients with SARS-CoV-2 infection and determine its efficacy and safety in reducing treatment failure events.

Methods: A retrospective cohort study involving patients with COVID-19 was conducted. Inverse probability of treatment weighting (IPTW) was used to balance covariates between the HBOT and non-HBOT groups.

Stimuli-Responsive Photoluminescent Molecular Tweezers for Highly Enantioselective Discrimination of Chiral Primary Amines.

To address the challenge of chiral recognition in terms of efficiency and generality, we propose a novel fluorescence sensing approach by rationally designing metal-ion-responsive chiral molecular tweezers. The flexible and adaptable molecular tweezers enable facile recognition of 31 structurally varied chiral primary amine compounds, including amino acids, amino acid esters, and chiral amines. Notably, upon stimulation by zinc ions, the chiral molecular tweezers demonstrate a higher enantioselective fluorescence response.

Amelioration of metabolic and behavioral defects through base editing in the Pah phenylketonuria mouse model.

Phenylketonuria (PKU) is a liver metabolic disorder mainly caused by a deficiency of the hepatic phenylalanine hydroxylase (PAH) enzyme activity, often leading to severe brain function impairment in patients if untreated or if treatment is delayed. In this study, we utilized dual-AAV8 vectors to deliver a near PAM-less adenine base editor variant, known as ABE8e-SpRY, to treat the Pah PKU mouse model carrying a frequent R408W mutation in the Pah gene. Our findings revealed that a single intravenous injection in adult mice and a single intraperitoneal injection in neonatal mice resulted in 19.

Seasonal dynamics of soil microbiome in response to dry-wet alternation along the Jinsha River Dry-hot Valley.

Background: Soil microorganisms play a key role in nutrient cycling, carbon sequestration, and other important ecosystem processes, yet their response to seasonal dry-wet alternation remains poorly understood. Here, we collected 120 soil samples from dry-hot valleys (DHVs, ~ 1100 m a.s.

Enhancing Tumor Immunity with IL-12 and PD-1 Blockade: A Strategy for Inducing Robust Central Memory T Cell Responses in Resistant Cancer Model.

Although immune checkpoint inhibitors (ICIs) have demonstrated efficacy in treating advanced cancers, their therapeutic success remains limited for many patients, with initial responders often experiencing resistance and relapse. Interleukin-12 (IL-12) is a powerful cytokine for antitumor immunotherapy, enhancing both lymphocyte recruitment into tumors and immune cell activation. In this study, we successfully produced mouse interleukin-12 (mIL12) through eukaryotic recombinant expression.

Human umbilical cord mesenchymal stem cells mitigate A1 astrocyte neuroinflammation induced by 1,2-dichloroethane via ERBB pathway inhibition.

1,2-Dichloroethane (1,2-DCE), a prevalent industrial and environmental contaminant, induces toxic encephalopathy through inhalation, leading to neurotoxic effects and inflammation-driven brain edema. Human umbilical cord mesenchymal stem cells (HUCMSCs) secrete bioactive factors, including miRNAs, proteins, and lipids via exosomes, exhibiting anti-inflammatory and immune-regulatory properties. However, their potential in treating 1,2-DCE-induced neuroinflammation and the underlying mechanisms remain unclear.

SynNotch-Programmed Macrophages for Cancerous Cell Detection and Sensing.

Synthetic Notch (synNotch) receptors have enabled mammalian cells to sense extracellular ligands and respond by activating user-prescribed transcriptional programs. Based on the synNotch system, we describe a cell-based in vivo sensor for cancerous cell detection. We attempted to engineer synNotch-programmed macrophages to sense cancer cells via urinary analysis of human chorionic gonadotropin (HCGB5).

User-wise perturbations for user identity protection in EEG-based BCIs.

Objective: An electroencephalogram (EEG)-based brain-computer interface (BCI) is a direct communication pathway between the human brain and a computer. Most research so far studied more accurate BCIs, but much less attention has been paid to the ethics of BCIs. Aside from task-specific information, EEG signals also contain rich private information, e.

Association between home literacy environment and eye movements during storybook reading: Implications for vocabulary and word reading in Chinese preschoolers.

This study explored the impact of different components of the Home Literacy Environment (HLE) -stimulation to use language, reading books and cultural engagement, joint activities and conversation, interactive reading, and zone-of-proximal development stimulation-on Chinese preschoolers' eye movements during storybook reading. Eighty-seven children aged 4-6 were assessed for vocabulary and word reading skills, while their eye movements were tracked during storybook reading. Their parents completed a questionnaire on personal background and frequency of HLE activities.

ST3GAL4 promotes tumorigenesis in breast cancer by enhancing aerobic glycolysis.

Sialyltransferases are enzymes that play a crucial role in regulating cancer progression by modifying glycoproteins through sialylation. In particular, the ST3 beta-galactoside alpha-2,3-sialyltransferase 4 (ST3GAL4) enzyme is known to be upregulated in breast cancer, but its specific biological functions have not been fully understood. This study aimed to investigate the impact and mechanisms of ST3GAL4 on aerobic glycolysis in breast cancer.

HBsAg and TLR7/8 dual-targeting antibody-drug conjugates induce sustained anti-HBV activity in AAV/HBV mice: a preliminary study.

Unlabelled: Hepatitis B virus (HBV) infection is a significant global health concern due to elevated immunosuppressive viral antigen levels, the host immune system's inability to manage HBV, and the liver's immunosuppressive conditions. While immunotherapies utilizing broadly reactive HBV neutralizing antibodies present potential due to their antiviral capabilities and Fc-dependent vaccinal effects, they necessitate prolonged and frequent dosing to achieve optimal therapeutic outcomes. Toll-like receptor 7/8 (TLR7/8) agonists have been demonstrated promise for the cure of chronic hepatitis B, but their systemic use often leads to intense side effects.