MicroRNA-182 Suppresses Malignant Melanoma Proliferation by Targeting RECK.

Background: MicroRNAs (miRNAs) function as important post-transcriptional regulators involved in a wide range of biological behaviors. MicroRNA-182 (miR-182) has been shown to play a critical role in tumor pathogenesis. The present study aimed to investigate the role of miR-182 in malignant melanoma.

COVID-19 Transmission Within a Family Cluster by Presymptomatic Carriers in China.

We report a family cluster of coronavirus disease 2019 (COVID-19) caused by a presymptomatic case. There were 9 family members, including 8 laboratory-confirmed with COVID-19, and a 6-year-old child had no evidence of infection. Among the 8 patients, 1 adult and a 13-month-old infant were asymptomatic, and 1 adult was diagnosed as having severe pneumonia.

Nanotechnology Strategies for the Analysis of Circulating Tumor DNA: A Review.

Circulating tumor DNA (ctDNA) describes the fragmented DNA released from tumor cells into the blood. The ctDNA may have the same genetic changes as the primary tumor. Currently, ctDNA has become a popular biomarker for diagnosis, treatment, real-time clinical response monitoring, and prognosis, for solid tumors.

Enhancing therapeutic efficacy of oncolytic vaccinia virus armed with Beclin-1, an autophagic Gene in leukemia and myeloma.

Different strategies were taken to make virotherapy more effective at killing cancer cells. Among them, oncolytic virus which arms the therapeutic gene to enhance antitumor activity is a prevalent approach. In this study, a newly developed oncolytic vaccinia virus (OVV) that expresses Beclin-1 (OVV-BECN1) was tested for its in vitro and in vivo oncolytic activity in blood cancer.

Long non-coding RNA RHPN1-AS1 promotes tumorigenesis and metastasis of ovarian cancer by acting as a ceRNA against miR-596 and upregulating LETM1.

Background: In recent decades, long non-coding RNAs (lncRNAs) have been reported as crucial functional regulators involved in ovarian cancer. In the present study, we explored how lncRNA RHPN1-AS1 influences the progression of epithelial ovarian cancer (EOC) through tumor cell-dependent mechanisms.

Results: The expression of RHPN1-AS1 in EOC tissues was higher than that in para-cancerous control tissues.

The alterations of mitochondrial DNA in coronary heart disease.

Coronary heart disease (CHD) is the major cause of death in modern society. CHD is characterized by atherosclerosis, which could lead to vascular cavity stenosis or obstruction, resulting in ischemic cardiac conditions such as angina and myocardial infarction. In terms of the mitochondrion, the main function is to produce adenosine triphosphate (ATP) for cells.

Post-dilatation improves stent apposition in patients with ST-segment elevation myocardial infarction receiving primary percutaneous intervention: A multicenter, randomized controlled trial using optical coherence tomography.

Background: Stent failure is more likely in the lipid rich and thrombus laden culprit lesions underlying ST-segment elevation myocardial infarction (STEMI). This study assessed the effectiveness of post-dilatation in primary percutaneous coronary intervention (pPCI) for acute STEMI.

Methods: The multi-center POST-STEMI trial enrolled 41 consecutive STEMI patients with symptom onset <12 hours undergoing manual thrombus aspiration and Promus Element stent implantation.

Determination of Chemical Oxygen Demand in Water Samples Using Gas-phase Molecular Absorption Spectrometry.

Chemical oxygen demand (COD) is important for water quality assessment as it represents the level of reductive organic pollution from eutrophication in aquatic systems. For surface water quality monitoring, permanganate is usually applied as an oxidizing reagent, and the routine COD determination is mostly achieved by titration method. However, this titration method is tedious and time consuming, and the results suffer from environmental temperature fluctuations and complicated operation techniques.

Association of the Polymorphism rs13259960 in SLEAR With Predisposition to Systemic Lupus Erythematosus.

Objective: Genome-wide association studies have identified many susceptibility loci for systemic lupus erythematosus (SLE). However, most of these loci are located in noncoding regions of the genome. Long noncoding RNAs (lncRNAs) are pervasively expressed and have been reported to be involved in various diseases.

Correction to: Ghrelin Fights against Titanium Particle-Induced Inflammatory Osteolysis through Activation of β-Catenin Signaling Pathway.

The original version of this article was published with incorrect Fig. 1B. The correct Fig.

Measurement of Retinal Changes in Primary Acute Angle Closure Glaucoma under Different Durations of Symptoms.

Purpose: To investigate the changes of retinal nerve fiber layer (RNFL) in patients after an attack of primary acute angle closure glaucoma (PAACG) and to assess the impact of attack time on prognosis of retinal changes.

Design: cross-sectional study.

Methods: Twenty-six patients with unilateral PAACG attack and cataracts from 2017 to 2019 were enrolled.

Evaluation of the diagnostic efficacy of F-Fluorine-2-Deoxy-D-Glucose PET/CT for lung cancer and pulmonary tuberculosis in a Tuberculosis-endemic Country.

Objective: To determine the diagnostic efficacy of F-FDG PET/CT in distinguishing between pulmonary tuberculosis (PTB) and lung cancer in solitary pulmonary nodule (SPN) in a country with a high prevalence of PTB.

Methods: Patients with SPN who underwent F-FDG PET/CT imaging were retrospectively included in the study. The final diagnosis was established by histopathology.

SPECT/CT Demonstrating 131I Accumulation in Gallbladder Stone in a Thyroid Cancer Patient.

The radioiodine in the gallbladder usually mimics focal hepatic activity on the planar I whole-body scintigraphy. Here we describe an abnormal uptake in the hepatic region on postablative I scintigraphy in a 37-year-old woman with papillary thyroid cancer. The SPECT/CT images revealed this "hepatic activity" corresponded to gallbladder stone.

Differences in the responses of flow and nutrient load to isolated and coupled future climate and land use changes.

Understanding the differences in the responses of river hydrology and water quality to climate and land use changes is particularly crucial for the development and management of water resources in the future. This study was carried out to assess the isolated and coupled effects of future climate change and land use change on the flow and nutrient load of the Xitiaoxi watershed in southeast China by applying the calibrated Hydrological Simulation Program Fortran model. Four representative concentration pathways released by the Intergovernmental Panel on Climate Change and two projected land use change scenarios were used to simulate future conditions.

Association between sexual intercourse frequency and pelvic floor muscle morphology in pregnant women.

Introduction And Hypothesis: No studies have been performed to examine the association of sexual activity and pelvic floor muscle (PFM) morphology or function during pregnancy. The aim of this study was to survey the frequency of sexual intercourse and examine the associations between the frequency of sexual intercourse and the PFM morphology in pregnant women of mainland China. The relationship between sexual intercourse frequency and stress urinary incontinence (SUI)-related symptoms was also evaluated.

Effects of wheat straw derived biochar on cadmium availability in a paddy soil and its accumulation in rice.

This study was carried out to investigate the effect of biochar amendment on cadmium (Cd) availability in a paddy soil with biochar amendment and its effect on the accumulation in rice. Biochar was applied once at rates of 0 (A0), 10 (A10), 20 (A20), 30 (A30), and 40 (A40) t ha on the soil surface layer (0-17 cm). Results showed that the soil organic matter (SOM) content and pH in 0-17 cm soil layer increased as biochar application rate increased, whereas the content of dissolved organic carbon (DOC), and available iron (Fe), manganese (Mn), aluminum (Al) in diethylenetriamine pentaacetic acid (DTPA) extracts declined with biochar added.

Machine Learning for Prioritization of Thermostabilizing Mutations for G-Protein Coupled Receptors.

Although the three-dimensional structures of G-protein coupled receptors (GPCRs), the largest superfamily of drug targets, have enabled structure-based drug design, there are no structures available for 87% of GPCRs. This is due to the stiff challenge in purifying the inherently flexible GPCRs. Identifying thermostabilized mutant GPCRs via systematic alanine scanning mutations has been a successful strategy in stabilizing GPCRs, but it remains a daunting task for each GPCR.

MiR-92a regulates brown adipocytes differentiation, mitochondrial oxidative respiration, and heat generation by targeting SMAD7.

Brown adipocytes are rich in mitochondria and linked to the body's blood fat levels and obesity. MiR-92a is negatively correlated with the activity of brown adipocytes. This study aimed to explore the mechanism of miR-92a on brown adipocytes.

Exosomal miRNAs as potential biomarkers for acute myocardial infarction.

microRNAs (miRNAs) can be used as biomarkers for acute myocardial infarction (AMI). However, few reports have focused on the value of exosomal miRNAs in the mechanism of the pathophysiological process from stable coronary artery disease (SCAD) to AMI. Exosomes were isolated via ultracentrifugation after serum samples were collected.

NPInter v4.0: an integrated database of ncRNA interactions.

Noncoding RNAs (ncRNAs) play crucial regulatory roles in a variety of biological circuits. To document regulatory interactions between ncRNAs and biomolecules, we previously created the NPInter database (http://bigdata.ibp.

MYC predetermines the sensitivity of gastrointestinal cancer to antifolate drugs through regulating TYMS transcription.

Background: Thymidylate synthase (TYMS) is a successful chemotherapeutic target for anticancer therapy. Numerous TYMS inhibitors have been developed and used for treating gastrointestinal cancer now, but they have limited clinical benefits due to the prevalent unresponsiveness and toxicity. It is urgent to identify a predictive biomarker to guide the precise clinical use of TYMS inhibitors.

Association of GCK gene DNA methylation with the risk of clopidogrel resistance in acute coronary syndrome patients.

Backgrounds: Clopidogrel resistance (CR), which was manifested as the failure of platelet inhibition in clopidogrel treatment, was likely to lead to cardiovascular events. Our study was aimed to explore the contribution of DNA methylation in glucokinase (GCK) to the CR risk.

Methods: Among 36 CR and 36 non-CR acute coronary syndrome (ACS) patients, the platelet functions were evaluated by VerifyNow P2Y12 assay (turbidimetric-based optical detection) and DNA methylation levels on two fragments of the CGI from the GCK were investigated through bisulfite pyrosequencing methods.

A male-specific association between AGTR1 hypermethylation and coronary heart disease.

The AGTR1 gene encodes angiotensin II receptor type 1, which is involved in cardiovascular diseases such as coronary heart disease (CHD). In the current study, we analyzed AGTR1 methylation level in a Han Chinese population by SYBR green-based quantitative methylation-specific PCR (qMSP). We collected blood samples from 761 CHD patients and 398 non-CHD controls at the Ningbo First Hospital.

Silk-Fibroin-Assisted Cathodic Electrolytic Deposition of Calcium Phosphate for Biomedical Applications.

The biological responses of artificial biomaterials are affected strongly by its interfacial topography, especially from nano- to microscale. The structure controllability is essential for the design and synthesis of novel biomedical materials. Herein, the effect of soluble silk fibroin (SF) on the cathodic electrolytic deposition (ELD) behavior of octacalcium phosphate (OCP) coating is studied.