Flexibility-Induced Collective Behavior Drives Symmetry Breaking in Discrimination of Undesired Ions.

Structure flexibility is essential for the biological function of proteins. At the same time, many proteins need to discriminate ligands with subtle differences, with one example being ion selectivity. Investigating the mechanisms by which flexible proteins achieve such precise discrimination is crucial for advancing our understanding of their functions.

Decoding Protein Glycosylation by an Integrative Mass Spectrometry-Based Sequencing Strategy.

Glycoproteins, representing more than 50% of human proteins and most biopharmaceuticals, are crucial for regulating various biological processes. The complexity of multiple glycosylation sites often leads to incomplete sequence coverage and ambiguous glycan modification profiles. Here, we developed an integrative mass spectrometry-based approach for decoding unknown glycoproteins, which is featured with the combination of deglycosylation-mediated sequencing with glycosylation site characterization.

Comparing between virtual reality based pre-clinical implantation training and traditional learning methods.

Objective: As dental implanting becomes an increasing demand among patients with tooth loss, an efficient and effective training for students is to be necessary. In this case, we anticipate the possible application of virtual reality (VR) technology to pre-clinical implantation training (PCIT) in order to improve the students' learning efficiency and effectiveness.

Methods: The study divided 20 subjects into two groups on average-VR based PCIT (experimental group) and traditional PCIT (control group) with the completion of the background survey (BS) before PCITs, to guarantee no apparent backgroud variation including learning of oral implantology and VR technology, learning habits, interests and hobbies, .

Preparation and characterization of electrically conductive nano-ZnO-doped Ti/Zr composite conversion coating on aluminum alloy.

The integration of semiconductive nano-ZnO into a Ti/Zr-based solution has facilitated the development of a conductive coating on aluminum alloy surfaces. A comprehensive characterization of the coating's morphology, microstructure, electrical conductivity, and corrosion resistance was conducted utilizing a suite of analytical techniques, including SEM, FIB-SEM, EDS, XPS, UV-vis, FTIR, and an electrochemical workstation. Significantly, the electrical contact resistance (ECR) of the coating experienced a substantial decrease when subjected to a pressure of 200 psi, plummeting from 0.

Decoding the Molecular Landscape of Prepubertal Oocyte Maturation: GTPBP4 as a Key Driver of In Vitro Developmental Competence.

The intricate mechanisms driving oocyte maturation remain only partially understood, especially within the domains of domestic animal reproduction and translational medicine. In the case of prepubertal girls, the clinical challenge is especially pronounced, as ovarian tissue cryopreservation-though promising-remains an experimental technique necessitating rigorous scientific validation to guarantee the developmental potential of preserved materials and facilitate broader clinical adoption. To address these knowledge gaps, while considering the ethical implications, we applied transcriptome and translatome sequencing to comprehensively profile the transcriptional and translational dynamics of oocyte maturation in adult and prepubertal goats.

SMUM211204 Exopolysaccharides Have Tumor-Suppressive Effects on Colorectal Cancer by Regulating Autophagy via the mTOR Pathway.

Probiotics have demonstrated their ability to suppress tumors in cell lines and mouse models. However, the precise molecules responsible for these effects remain unidentified. We focused on isolating and analyzing the exopolysaccharides (EPSs) produced by () SMUM211204.

[Clinical and Laboratory Characteristics of Streptococcus mitis Causing Bloodstream Infection in Children with Hematological Disease].

Objective: To investigate the risk factors, clinical characteristics, and bacterial resistance of bloodstream infections caused by in children with hematological disease, so as to provide a reference for infection control.

Methods: The clinical information and laboratory findings of pediatric patients complicated with blood cultures positive for from January 2018 to December 2020 in the Institute of Hematology & Blood Diseases Hospital were searched and collected. The clinical characteristics, susceptibility factors, and antibiotic resistance of the children were retrospectively analyzed.

TIP2-UDT1-OsUPEX1/2 module regulates tapetum development and function in rice.

The tapetum in the anther wall is essential for plant fertility, secreting many components essential for pollen development. Development of the tapetum is controlled by multiple transcription factors and signaling pathways. UDT1, TIP2, TDR, and EAT1 constitute a sequential regulatory cascade crucial for tapetal differentiation in rice, but UDT1- and TIP2-dependent regulatory networks, particularly in early anther development, remain largely unknown.

Sea buckthorn flavonoids protect RPE cells from UVB-induced oxidative damage.

Natural flavonoids are believed to provide protection to retinal pigment epithelial (RPE) cells due to their well-established antioxidant properties. This study aims to evaluate the impact of sea buckthorn flavonoids on a UVB-induced oxidative damage model of RPE cells. The n-butanol extract fraction (Fr.

Bone marrow fluid enhances the osteogenic activity of induced membrane leading to spontaneous osteogenesis: experimental validation and application in tibiofibular fusion for support reconstruction of segmental tibial defects.

Background: Managing large bone defects remains a significant clinical problem. We enhanced the osteogenic activity of the induced membrane (IM) by incorporating bone marrow fluid, leading to spontaneous osteogenesis (SO). We aimed to explore the application of this method in tibiofibular fusion (TFF) for reconstructing segmental tibial defects.

Improving activity of GenB3 and GenB4 in gentamicin dideoxygenation biosynthesis by semi-rational engineering.

Background: Aminoglycoside antibiotics continue to play an indispensable role in clinical antibacterial agents. However, the protection and deprotection procedures in the chemical pathways of semi-synthetic antibiotics are long, atom- and step-inefficient, which severely hampers the development of novel AGs.

Results: Here, GenB3 and GenB4 are employed to synthesize sisomicin, Oxo-verdamicin, Oxo-gentamicin C1a, and Oxo-gentamicin C2a.

Pectin-Zein-IPA nanoparticles promote functional recovery and alleviate neuroinflammation after spinal cord injury.

Introduction: Spinal cord injury (SCI) impairs the balance of gut microbiomes, which further aggravates inflammation in the injured areas and inhibits axonal regeneration. The intestinal microbiome plays an important role in SCI and regulating intestinal microbiome promotes SCI repair. However, current studies have shown that indole-3 propionate (IPA), a metabolite of gut bacteria, can promote axonal regeneration.

Prediction of sarcopenia at different time intervals: an interpretable machine learning analysis of modifiable factors.

Objectives: This study aims to develop sarcopenia risk prediction models for Chinese older adults at different time intervals and to identify and compare modifiable factors contributing to sarcopenia development.

Methods: This study used data from 3,549 participants aged 60 and older in the China Health and Retirement Longitudinal Study (CHARLS). Sarcopenia status was evaluated by the AWGS2019 algorithm.

Associations of composite dietary antioxidant index with premature death and all-cause mortality: a cohort study.

Although previous evidence indicates that the composite dietary antioxidant index (CDAI) is negatively associated with health outcomes, no studies have explored the association between CDAI and premature death. This research utilized a cohort study design with 37,301 participants from the National Health and Nutrition Examination Survey (NHANES) from 2003 to 2018. Cox proportional hazard regression was employed to analyze the association between CDAI and premature death and all-cause mortality.

Pre- and post-diagnosis dietary patterns and overall survival in patients with epithelial ovarian cancer: a prospective cohort study.

Background: Previous studies have examined the associations between individual foods or nutrients, but few studies have considered dietary patterns associated with ovarian cancer (OC) survival.

Methods: In a prospective cohort study, we examined the association between pre-diagnosis and post-diagnosis overall diet, including changes from pre-diagnosis to post-diagnosis, and overall survival (OS) in 560 patients with OC. Dietary intake was collected using a valid 111-item food frequency questionnaire.

Operation sequence of bidirectional endoscopy with topical anesthesia affected colonoscopy performance: a randomized controlled trial.

Background: The operation sequence of bidirectional endoscopy with topical anesthesia varies among endoscopists, which interferes with clinical procedure. The study aimed to investigate the influence of different operation sequences on the outcomes of quality indicators and improve bidirectional endoscopy performance.

Methods: A randomized trial was conducted at the affiliated hospital of a medical university in China.

Autoregulatory-guided management in traumatic brain injury: does age matter?

Background: Although older traumatic brain injury (TBI) patients often exhibit cerebral autoregulatory impairment with high pressure reactivity index (PRx), the role of autoregulatory-guided management in these patients remains elusive. In this study, we aimed to explore if age affected the prognostic role of the autoregulatory variables, PRx and the PRx-derived optimal cerebral perfusion pressure (CPPopt), in a large TBI cohort.

Methods: In this observational study, 550 TBI patients who had been treated in the neurocritical care unit, Addenbrooke's Hospital, Cambridge, UK, between 2002 and 2022 with available data on age, intracranial pressure monitoring, and outcome (Glasgow Outcome Scale [GOS]) were included.

A deep learning based ultrasound diagnostic tool driven by 3D visualization of thyroid nodules.

Recognizing the limitations of computer-assisted tools for thyroid nodule diagnosis using static ultrasound images, this study developed a diagnostic tool utilizing dynamic ultrasound video, namely Thyroid Nodules Visualization (TNVis), by leveraging a two-stage deep learning framework that involved three-dimensional (3D) visualization. In this multicenter study, 4569 cases were included for framework development, and data from seven hospitals were employed for diagnostic validation. TNVis achieved a Dice similarity coefficient of 0.

The surgical technique and efficacy of a novel pure retroperitoneoscopic extravesical bladder cuff excision without intraoperative repositioning for upper tract urothelial carcinoma.

To evaluate the feasibility, effectiveness and therapeutic outcomes of a novel pure retroperitoneoscopic extravesical bladder cuff excision (PREBE) in patients with upper tract urothelial carcinoma (UTUC), a retrospectively comparative study between PREBE and traditional laparoscopic nephroureterectomy (TLNU) was done in a single center. We retrospectively evaluated 72 patients diagnosed with UTUC at our hospital from January 2022 to January 2024. The patients who underwent TLNU (n = 35) and PREBE (n = 37) were consecutively enrolled.

Molecular HDD logic for encrypted massive data storage.

Organic memories, with small dimension, fast speed and long retention features, are considered as promising candidates for massive data archiving. In order to satisfy the requirements for ultra-low power and high-security information storage, we design a conceptual molecular hard-disk (HDD) logic scheme that is capable to execute in-situ encryption of massive data in pW/bit power-consumption range. Beneficial from the coupled mechanism of counter-balanced redox reaction and local ion drifting, the basic HDD unit consisting of ~200 self-assembled RuLPH molecules in a monolayer (SAM) configuration undergoes unique conductance modulation with continuous, symmetric and low-power switching characteristics.

A phase 3 clinical trial on the immunogenicity and safety of booster vaccination after Zagreb or Essen regimens.

The Zagreb and Essen regimens are comparable in terms of immunogenicity; however, how these regimens impact the immunogenicity of booster vaccination remains unknown. We conducted a clinical trial to assess the immunogenicity of booster vaccination with a purified Vero cell rabies vaccine after primary vaccination with the Zagreb or Essen regimens. This randomized, open-label, controlled, phase 3 trial, conducted in Shangyu City and Shengzhou City, Zhejiang Province, China, was divided into the primary and booster vaccination phases.

Finite element analysis of flexural performance of reinforced truss hollow composite concrete slabs.

Combining the advantages of cast-in-place hollow slabs and prefabricated reinforced truss composite concrete slabs, a novel hollow composite slab is proposed, characterized by the inclusion of hollow thin-walled boxes without reinforcement at the edges, referred to as the hollow composite slab. To further investigate the flexural performance and critical design parameters of the hollow composite slab, numerical simulations were conducted using the finite element software ABAQUS. Based on the actual specimen fabrication and test results, the rationality of the finite element modeling was validated.

Palladium-Catalyzed α-Arylation of Sulfoxonium Ylides with Aryl Thianthrenium Salts via C-S and C-H Bond Activation.

Diverse α-aryl α-carbonyl sulfoxonium ylides were efficiently synthesized in yields ranging from moderate to high via a palladium-catalyzed α-arylation of sulfoxonium ylides with aryl thianthrenium salts. The reactions proceeded smoothly via C-S and C-H bond functionalization, exhibiting broad substrate scope and good compatibility to various functionalities. In addition, the scale-up synthesis could be achieved, and the one-pot protocol commencing from the use of simple arene as the precursor of aryl thianthrenium salt could also be accomplished.