Vasodilation effect of 2-benzyl-5-hydroxy-6-methoxy-3, 4-dihydroisoquinolin-1-one.

A 2-Benzyl-5-hydroxy-6-methoxy-3, 4-dihydroisoquinolin-1-one (ZC2) is a newly synthesized isoquinolinone compound. Its effect on vasodilation was evaluated in the present study. Isometric tension of rat artery rings was recorded by a sensitive myography system in vitro.

The structure, density and settlability of anammox granular sludge in high-rate reactors.

Microscopic observation and settling test were carried out to investigate the structure, density and settlability of anammox granules taken from a high-rate upflow anaerobic sludge blanket (UASB) reactor. The results showed that the anammox granules were irregular in shape and uneven on surface, and their structure included granule, subunit, microbial cell cluster and single cell. The gas pockets were often observed in the anammox granules, and they originated from the obstruction of gas tunnel by extracellular polymer substances (EPSs) and the inflation of produced dinitrogen gas.

Growth and metabolism characteristics of anaerobic ammonium-oxidizing bacteria aggregates.

The present study reported the growth and metabolism characteristics of anaerobic ammonium-oxidizing (anammox) bacteria aggregates in an expanded granular sludge bed (EGSB). The results showed that the anammox bacteria aggregates presented starvation, growth, and inhibition phase along with the increase of substrate supply. The substrate conversion rates for survival were 0.

Surgical treatment of isolated right-sided infective endocarditis.

We reviewed our department's experience with the perioperative features and surgical treatment of isolated right-sided infective endocarditis. From January 2000 through July 2010, 35 patients underwent surgery for isolated right-sided infective endocarditis in our department. The mean pathologic course was 3.

catena-Poly[[(3-methyl-pyridine)-copper(I)]-μ-cyanido-copper(I)-μ-cyanido].

In the title complex, [Cu(2)(CN)(2)(C(6)H(7)N)](n), there are two copper atoms with different coordination environments. One Cu atom (Cu1) is linked to the two cyanide ligands, one N atom from a pyridine ring while the other (Cu2) is coordinated by the two cyanide ligands in a slightly distorted tetra-hedral geometry and linked to Cu1, forming a triangular coordination environment. The Cu atoms are bridged by bidentate cyanide ligands, forming an infinite Cu-CN chain.

trans-Diaqua-bis-[4-carboxy-5-carboxyl-ato-2-(pyridin-1-ium-4-yl)-1H-imidazol-1-ido-κN,O]cobalt(II).

In the title compound, [Co(C(10)H(6)N(3)O(4))(2)(H(2)O)(2)], the Co(II) ion is coordinated by two O atoms of two water mol-ecules, two imidazole nitro-gen atoms and two carboxyl-ate O atoms of the two trans-standing chelate ligands, displaying a distorted octa-hedral coordination geometry. A three-dimensional supra-molecular framework is generated through N-H⋯O, O-H⋯N and O-H⋯O hydrogen-bonding inter-actions.

Aqua-(pyridine-3-carb-oxy-lic acid-κN)(pyridine-2,6-dicarboxyl-ato-κO,N,O)copper(II) monohydrate.

In the title Cu(II) complex, [Cu(C(7)H(3)NO(4))(C(6)H(5)NO(2))(H(2)O)]·H(2)O, the environment of the Cu(2+) ion is a distorted square pyramid with the axial site occupied by the O atom from the coordinated water mol-ecule and the square base formed by two O and two N atoms from the tridentate anion and the neutral monodentate pyridine-3-carboxylic acid ligand. O-H⋯O hydrogen bonds, as well as π-π inter-actions [centroid-centroid distance = 3.945 (3) Å] contribute to the stabilization of this structure.

PML-RARα enhances constitutive autophagic activity through inhibiting the Akt/mTOR pathway.

Autophagy is a highly conserved, closely regulated homeostatic cellular activity that allows for the bulk degradation of long-lived proteins and cytoplasmic organelles. Its roles in cancer initiation and progression and in determining the response of tumor cells to anticancer therapy are complicated, and only limited investigation has been conducted on the potential significance of autophagy in the pathogenesis and therapeutic response of acute myeloid leukemia. Here we demonstrate that the inducible or transfected expression of the acute promyelocytic leukemia (APL)-specific PML-RARα, but not PLZF-RARα or NPM-RARα, fusion protein upregulates constitutive autophagy activation in leukemic and nonleukemic cells, as evaluated by hallmarks for autophagy including transmission electron microscopy.

[TfR2 mRNA expression in bone marrow mononuclear cells of children with hyperplastic anemia and its implications].

The aim of this study was to investigate the expression of transferrin receptor 2 (TfR2) mRNA in bone marrow mononuclear cells (BMMNC) of children with hyperplastic anemia (HA), to analyze the correlation of TfR2 mRNA expression level with Hb level, bone marrow erythroid hyperplasia, iron status in body and underlying diseases, and to evaluate the role of TfR2 in erythroid hemopoiesis and the useful value in diagnosis of HA. The experiment was divided into 2 groups: test group, in which 40 patients with HA were enrolled, and control group in which 10 patients without erythroid disorders and hematological malignancies confirmed by bone marrow examination were enrolled. The bone marrow samples of patients in mentioned above 2 groups were collected, the TfR2 mRNA expression in BMMNC of patients with HA was detected by fluorescence-quantitative PCR, the correlation of HA with bone marrow erythroid hyperplasia, iron status of body and underlying diseases was analyzed.

[Analysis on the mental disorders in the elderly after falling and its influencing factors].

Objective: To explore the prevalence and its influencing factors on mental disorders in older people after falling in a community from Guangzhou city.

Methods: 184 people over 60 years of age had ever fallen within the past year in a community, were investigated by structured interview survey on their mental disorders in Guangzhou, with the impact of event scale (IES) and fear fall scale (FFS). Another 56 people over the age of 60 had never fallen at the same time were selected as controls.

Destructive pulmonary staphylococcal infection in a boy with hyper-IgE syndrome: a novel mutation in the signal transducer and activator of transcription 3 (STAT3) gene (p.Y657S).

Unlabelled: Hyper IgE syndrome (HIES) is a rare primary immunodeficiency disorder, characterized by eczema, recurrent skin and lung infections, and significantly elevated serum IgE level. It was previously diagnosed based on clinical manifestations and laboratory markers that were not specific to the disease. Recent studies have demonstrated that mutations in signal transducer and activator of transcription 3 (STAT3) cause the autosomal dominant or sporadic HIES, which make the disease definitively characterized at molecular level.

Enhanced nitrogen removal from pharmaceutical wastewater using SBA-ANAMMOX process.

Efficient biological nitrogen removal from pharmaceutical wastewater has been focused recently. The present study dealt with the treatment of colistin sulfate and kitasamycin manufacturing wastewater through anaerobic ammonium oxidation (ANAMMOX). The biotoxicity assay on luminescent bacterium Photobacterium phosphoreum (T3 mutation) showed that the pharmaceutical wastewater imparted severe toxicity with a relative luminosity of 3.

In vitro and in vivo apoptosis-inducing antileukemic effects of Mucuna macrocarpa stem extract on HL-60 human leukemia cells.

Mucuna macrocarpa Wallich (Leguminosae) is believed to hold blood circulation activating effects, and has been used as a folk remedy in Southeast Asia for the treatment of various hematologic and circulatory-related ailments. The objective of this study was to investigate whether crude methanolic extract of M macrocarpa (CMEMM) possessed antileukemic effects on HL-60, human leukemia cells. CMEMM was prepared from dried stems of this plant, and its apoptosis-inducing effects were investigated using HL-60 cells in vitro and in vivo.

Protective effects of omega-3 fish oil on lead-induced impairment of long-term potentiation in rat dentate gyrus in vivo.

In order to evaluate the effect of omega-3 fish oil supplement by gavage (0.4 mL/100 g body weight) on the chronic lead-induced (0.2% lead acetate) impairments of long-term potentiation (LTP) in rat dentate gyrus (DG) in vivo, we designed the experiments which were carried out in four groups of newborn Wistar rats (the control, the lead-exposed, the control with fish oil treatment and the lead-exposed with fish oil treatment, respectively).

Hypoxia inducible factor-1 mediates expression of galectin-1: the potential role in migration/invasion of colorectal cancer cells.

The expression of galectin-1, one of the most important lectins participating in the malignant tumor development, has been shown to be regulated by hypoxia, but its exact mechanism remains elusive. Here, we find that ectopically expressed hypoxia-inducible factor (HIF) 1alpha protein, an oxygen-sensitive subunit of HIF-1 that is a master factor for cellular response to hypoxia, significantly increases galectin-1 expression in both messenger RNA and protein levels in all four colorectal cancer (CRC) cell lines tested. However, hypoxia-induced galectin-1 expression cannot be seen in sentrin/SUMO-specific protease 1 homozygous-null mouse embryonic fibroblasts that fail to accumulate HIF-1alpha protein.

Sub-maximal exercise altered the prednisolone absorption pattern.

Purpose: Prednisolone has been widely used due to its relatively short pituitary inhibition and its moderate potency. Exercise is one of the non-pharmacological interventions for glucocorticoid- induced osteoporosis prevention. However, the effects of exercise on the pharmacokinetics of prednisolone were unclear.

[Expression of mitochondrial ferritin in K562 leukemic cell during ATRA-induced cell differentiation].

Objective: To investigate the expression of MtF, transferrin receptor 1 (TfR1) and ferritin (Fn) mRNAs in K562 leukemic cells during ATRA-induced cell differentiation and to explore the interrelationship between the expression levels of these iron metabolism-related molecules.

Methods: K562 cells cultured with or without ATRA (1 micromol/L) were collected at 24, 72 and 120 hours respectively. Cell differentiation toward granulocyte lineage was confirmed by microscopic study (Wright's staining) and flowcytometry.

Investigation of the interactions of lysozyme and trypsin with biphenol A using spectroscopic methods.

The interaction between bisphenol A (BPA) and lysozyme (or trypsin) was investigated by UV-vis absorption, fluorescence, synchronous fluorescence, and three-dimensional fluorescence spectra techniques under physiological pH 7.40. BPA effectively quenched the intrinsic fluorescence of lysozyme and trypsin via static quenching.

[Anesthesic management in telerobotic totally endoscopic coronary artery bypass grafting].

Objective: To investigate the strategies for anesthetic management for totally endoscopic coronary artery bypass grafting with the da Vinci S surgical system.

Methods: Between January 2007 and May 2009, 16 patients underwent off-pump coronary artery bypass grafting via a totally endoscopic approach. After anesthetic induction, a left-sided double-lumen endotracheal tube was positioned to allow single right-lung ventilation during intra-operative procedure.

[Species diversity and ecological distribution of anaerobic ammonium-oxidizing bacteria].

Anaerobic ammonium oxidation (anammox) is an important discovery in microbiology and environmental sciences, which can simultaneously remove NH4(+) -N and NO3(-) -N, being valuable in environmental engineering. However, anaerobic ammonium oxidizers are extremely slow-growing, and their population's doubling time is longer than 11 days, which seriously restricts the application of anammox process. Therefore, the study of anammox bacteria is of significance.

[Effects of pravastatin and granulocyto-colony stimulating factor in mobilizing endothelial progenitor cells in mice with myocardial ischemia].

Objective: To compare the effects of pravastatin and granulocyto-colony stimulating factor (G-CSF) in mobilizing endothelial progenitor cells (EPCs) in mice with myocardial ischemia, and explore the possible mechanism of EPC mobilization.

Methods: Ninety-six mice were randomly divided into 4 groups (n=24), namely the control group, saline group, pravastatin group and G-CSF group. In the latter 3 groups, myocardial ischemia was induced with isoprenine followed by intraperitoneal injections of normal saline, pravastatin and G-CSF for 5 consecutive days.

[Expression of transferrin receptor 2 in mononuclear cells from children with acute leukemia].

Objective: To study the expression of TFR2 mRNA in mononuclear cells of bone marrow and peripheral blood from children with acute lymphoblastic leukemia, and to explore possible correlations between TFR2 expression and a panel of prognostic/risk factors.

Methods: Bone marrow or peripheral mononuclear cells were isolated from 56 children with newly diagnosed acute lymphoblastic leukemia (ALL) and 15 normal children as control. TFR2 mRNA expression level in mononuclear cell was determined by real-time fluorescence quantitative RT-PCR.

[Inhibitory action of hydroxysafflor yellow A on inflammatory signal transduction pathway related factors in rats with cerebral cortex ischemia].

Hydroxysafflor yellow A (HSYA) is a main active monomer purified from Carthamus tinctorius L. The research is to study the inhibitory effect of HSYA on the inflammatory signal transduction pathway related factors which were induced by permanent cerebral ischemia in rats. By using the successive administration at a 30 min interval of HSYA and the rats permanent focal cerebral ischemia model established by a intraluminal suture occlusion method.