Root morphology, exudate patterns, and mycorrhizal symbiosis are determinants to improve phosphorus acquisition in alfalfa.

Differences in phosphorus (P) utilisation efficiency (PUtE) and/or yield are closely linked to differences in root functional traits under low soil P availability. However, our understanding of how soil P availability mediates the intraspecific variation in root functional traits for breeding high-P efficiency genotypes to increase PUtE and yield remains limited. We investigated that plant growth parameters and pivotal root functional traits associated with P acquisition in 20 alfalfa genotypes with contrasting P efficiencies and supplied with low, medium or high levels of P.

Long-term health-related quality of life in Kawasaki disease complicated with coronary artery aneurysm in the Nanjing region of China: Results of the largest single-center assessment.

Objectives: The study aimed to compare the long-term health-related quality of life in children with Kawasaki disease (KD) with and without coronary artery aneurysms (CAAs) in the largest pediatric medical center in the Nanjing region of China.

Patients And Methods: The retrospective study included a total of 107 patients (54 males, 53 females; mean age: 3.4±1.

Multi-omic stock of surface ocean microbiome built by monthly, weekly and daily sampling in Dapeng Bay, China.

The coastal ocean is the dynamic interface where terrestrial, atmospheric, and marine systems converge, acting as a hotspot for microbial activity, which underpins the intricate web of carbon and nitrogen cycling. Dapeng Bay, a typical semi-enclosed bay along the southern coastline of China, is strongly influenced by monsoon climates and human activities. Despite its ecological importance, long-term observations and investigations into the microbial community structure in this region are notably lacking.

Eliminating Radioresistance With a Magnetic Ion-Generator by Simultaneously Augmenting DNA Damage and Diminishing Immunosuppression.

Radiotherapy (RT) hinges on DNA damage-induced cancer cell death and the subsequent anti-tumor immunity. However, the efficacy of RT is curtailed by cell cycle heterogeneity and an immunosuppressive tumor microenvironment, which foster radioresistance. Here an ion generator-based RT enhancement strategy is demonstrated in a mouse model of the radioresistant tumor.

Identification of Epigenetic Alteration of the IFI44L Gene in B Cells of Sjogren's Syndrome as a Clinical Biomarker and Molecular Significance.

Objective: Type 1 interferon (IFN-I)-related genes play a critical role in Sjögren's syndrome (SS). However, study on its role and impact in peripheral blood B cells of SS is limited. This study investigated gene expression and epigenetic changes in IFI44L, analyzing its correlation with disease activity and clinical indicators.

A three-site recognition cytosensor for accurate detection of circulating tumor cells based on novel branched Pt Au nanospheres and MnO-GO-Au nanosheets.

A novel electrochemical cytosensor was developed with a three-protein recognition strategy, employing branched Pt Au nanospheres (B-Pt-Au NPs) as tags and MnO-GO-Au nanosheet-modified electrodes for circulating tumor cells (CTC) detection. This system integrates specific magnetic separation and CTC enrichment with sensitive electrochemical detection to identify rare A549 cells in whole blood. MnO-GO-Au nanosheets, known for their high conductivity and biocompatibility, were used to modify a bare electrode, improving the electrochemical interface for biomolecules.

Preliminary Field-Based Assessment of Disease Symptoms and Physical Wounds in Endangered Shennongjia Golden Snub-Nosed Monkey (Rhinopithecus roxellanahubeiensis) Colonies: Implications for Conservation.

Background: Globally, approximately 60% of primate taxa are in danger of extinction and the population of 75% of primate species are declining in their natural habitats. The Endangered R. roxellana hubeiensis monkeys in Shennongjia National Park face threats due to their limited genetic diversity and restricted habitat.

Small-area association of hourly ambient temperature and acute morbidity.

Previous studies have reported the association between daily temperature and morbidity at city level, but small-area association of hourly temperature and acute morbidity is limited. We aimed to assess the association between hourly temperature and acute morbidity at the smallest administrative units (communities), and to explore the sources of regional heterogeneity. This case time series design was conducted using 145,678 non-accidental ambulance dispatch records in 328 communities in Fuzhou, China (2019-2024).

Pre- and Post-Surgical MRI Analysis of Levator Ani in Pelvic Organ Prolapse Patients: A Single-Center Study.

BACKGROUND This single-center study involved 33 women diagnosed with pelvic organ prolapse (POP), aiming to evaluate the levator ani muscle (LAM) using pre- and post-surgery magnetic resonance imaging (MRI). MATERIAL AND METHODS MRI data of 33 women were analyzed before and after POP surgery. Diagnoses used the Pelvic Organ Prolapse Quantification (POP-Q) system, including 10 cases of stage II, 5 of stage III, and 18 of stage IV with pelvic organ prolapse.

Long-Term and Real-Time Post-External Radiotherapy Assessment Based on an In Situ Activatable Radiolabeled Platform.

Long-term monitoring in postoperative assessment is essential for clinicians to assess the effectiveness of therapies and establish subsequent clinical pharmacotherapeutic plans. However, precise and real-time postoperative assessment is often overlooked, relying instead on various clinical histopathological and cytological assays or the experience of physicians. Therefore, it is urgent to develop a general strategy for long-term, real-time, and accurate postoperative assessment.

Dual Thermally Activated Delayed Fluorescence from a Single Carbazole Derivative with Multiple Charge Transfer Pathways.

Two carbazole derivatives BPA-BNC and 4BPABNC are designed and synthesized to explore the probable dualemission behavior and mechanisms. BPA-BNC contains two arylamino donors and one B-N multi-resonance (MR) segment at 3,6,9-positions of a single carbazole, while 4BPABNC has two extraarylamine donors at 1,8-positions in addition to the above substituent groups. Two compounds in polar solvents show a clear dual emission peaked at near 490 and 540 nmwith high photoluminescence quantum yields of up to 98 %, short delayed lifetimesand extremely small single-triplet splitting energies.

Efficacy and safety of antifibrotic drugs for interstitial lung diseases other than IPF: A systematic review, meta-analysis and trial sequential analysis.

Background: The therapeutic role of antifibrotic therapy has been well-established in idiopathic pulmonary fibrosis (IPF). However, its efficacy and safety for interstitial lung diseases (ILDs) other than IPF are not fully understood.

Methods: We updated a systematic review with meta-analysis and trial sequential analysis (TSA) of randomized controlled trials and prospective studies on antifibrotic drug (nintedanib or pirfenidone) vs other intervention (placebo, no intervention or conventional treatment) in non-IPF ILDs.

Long-Term Health-Related Quality of Life in Kawasaki Disease-Related Coronary Artery Aneurysm: A Large Single-Center Assessment in Nanjing, China.

Background: The impact of coronary artery aneurysms (CAAs) caused by Kawasaki disease (KD) on long-term health-related quality of life (HRQOL) in children has not been well documented.

Methods: This study investigated long-term HRQOL in a large sample of children diagnosed with KD-related CAAs. A case-control, retrospective study included 66 patients with KD-related CAAs.

Harnessing crRNA Transformer for Facile and Specific Nucleic Acid Detection.

CRISPR/Cas systems have emerged as promising tools for nucleic acid detection. However, their practical applications have been limited by poor specificity and the need for additional preprocessing steps. Inspired by the concept of transformers, we found that changing the forms of crRNA with spatial arrangement may endow CRISPR/Cas with an enhanced performance for nucleic acid detection.

Moderate-To-Severe Bone Marrow Fibrosis Is an Independent Risk Factor For Myelodysplastic Neoplasms Patients With Increased Blasts.

This study, including 412 patients newly diagnosed with myelodysplastic neoplasm (MDS), investigated the clinical, molecular, and prognostic features of MDS with moderate-to-severe bone marrow fibrosis (MF). Among the patients with MDS, 347 (84%) had MF grade 0-1 (MF0-1), and 65 (16%) had MF grade 2-3 (MF2-3). Patients with MDS with MF2-3 showed similar overall survival (OS) (16.

EZH2 inhibition induces pyroptosis via RHA-mediated S100A9 overexpression in myelodysplastic syndromes.

Myelodysplastic Syndromes (MDS) represent a group of heterogeneous myeloid clonal diseases derived from aberrant hematopoietic stem/progenitor cells. Enhancer of zeste homolog 2 (EZH2) is an important regulator in gene expression through methyltransferase-dependent or methyltransferase-independent mechanisms. Herein, we found EZH2 inhibition led to MDS cell pyroptosis through RNA Helicase A (RHA) down-regulation induced overexpression of S100A9, a key regulator of inflammasome activation and pyroptosis.

Influence mechanism of serum free immunoglobulin light chain on pulmonary inflammatory response and serum levels of inflammatory factors in patients with chronic obstructive pulmonary disease.

Background: The pathogenesis of chronic obstructive pulmonary disease (COPD) is not fully understood. This work aimed to demonstrate the role of serum free light chains (sFLC) in the pulmonary inflammatory response of COPD patients and its relationship with serum inflammatory cytokine (IC) levels.

Methods: Eighty subjects were enrolled, including 40 COPD patients (COPD group) and 40 healthy controls (control group).

Lineage tracing studies suggest that the placenta is not a de novo source of hematopoietic stem cells.

Definitive hematopoietic stem and progenitor cells (HSPCs) arise from a small number of hemogenic endothelial cells (HECs) within the developing embryo. Understanding the origin and ontogeny of HSPCs is of considerable interest and potential therapeutic value. It has been proposed that the murine placenta contains HECs that differentiate into HSPCs.

Astragaloside IV ameliorates autism-like behaviors in BTBR mice by modulating Camk2n2-dependent OXPHOS and neurotransmission in the mPFC.

Introduction: Autism spectrum disorder (ASD) represents a multifaceted set of neurodevelopmental conditions marked by social deficits and repetitive behaviors. Astragaloside IV (ASIV), a natural compound derived from the traditional Chinese herb Astragali Radix, exhibits robust neuroprotective effects. However, whether ASIV can ameliorate behavioral deficits in ASD remains unknown.

A Time Course Analysis of the Conceptual and Affective Meanings of Words.

Words are the basic units of language and vital for comprehending the language system. Lexical processing research has always focused on either conceptual or affective word meaning. Previous studies have indirectly compared the conceptual and affective meanings of words.

Prediction of p-phenylenediamine antioxidant concentrations in human urine using machine learning models.

p-phenylenediamine antioxidants (PPDs) are extensively used in rubber manufacturing for their potent antioxidative properties, but PPDs and 2-anilino-5-[(4-methylpentan-2yl)amino]cyclohexa-2,5-diene-1,4-dione (6PPDQ) pose potential environmental and health risks. Existing biomonitoring methods for assessing human exposure to PPDs are labor-intensive, costly, and provide limited data. Thus, there is a critical need to develop predictive models for evaluating PPDs and 6PPDQ exposure levels to facilitate health risk assessments.

Dysregulated Signaling Pathways in Canine Mammary Tumor and Human Triple Negative Breast Cancer: Advances and Potential Therapeutic Targets.

In 2022, human breast cancer (HBC) and canine mammary tumors (CMTs) remained the most prevalent malignant tumors worldwide, with high recurrence and lethality rates, posing a significant threat to human and dog health. The development of breast cancer involves multiple signaling pathways, highlighting the need for effective inhibitory drugs that target key proteins in these pathways. This article reviews the dysregulation of the EGFR, PI3K/AKT/mTOR, Hippo, pyroptosis, and PD-1/PD-L1 signaling pathways in HBC and CMT, as well as the corresponding drugs used to inhibit tumor growth, with the aim of providing theoretical support for the development of more efficient drugs.

Synergistic antimicrobial efficacy of glabrol and colistin through micelle-based co-delivery against multidrug-resistant bacterial pathogens.

Background: Widespread bacterial infection and the spread of multidrug resistance (MDR) exhibit increasing threats to the public and thus require new antibacterial strategies. Coupled with the current slow pace of antibiotic development, the use of antibiotic adjuvants to revitalize existing antibiotics offers great potential.

Purpose: We aim to explore the synergistic antimicrobial mechanism of glabrol (GLA) and colistin (COL) while developing an innovative multifunctional micelle-based drug delivery system to enhance therapeutic efficacy.

Large Language Modeling to Assist Natural Polyphenols as Green Precipitants for Recycling Spent Batteries.

The growing demand for energy storage batteries, driven by the need to alleviate global warming and reduce fossil fuel dependency, has led to environmental concerns surrounding spent batteries. Efficient recycling of these batteries is essential to prevent pollution and recover valuable metal ions such as nickel (Ni), cobalt (Co), and manganese (Mn). Conventional hydrometallurgical methods for battery recycling, while effective, often involve harmful chemicals and processes.

A novel variant c.7104 + 6T > A of linked to autosomal recessive congenital ichthyosis verified by minigene splicing assay.

Background: Autosomal recessive congenital ichthyosis (ARCI) is a group of genetic skin disorders characterized by abnormal keratinization, leading to significant health issues and reduced quality of life. ARCI encompasses harlequin ichthyosis (HI), congenital ichthyosiform erythroderma (CIE), and lamellar ichthyosis (LI). While all ARCI genes are linked to LI and CIE, HI is specifically associated with severe mutations in the gene.